Life Cycle of Glomus Species in Monoxenic Culture

Dalpé, Yolande; Souza, F. A. de; Declerck, Stéphane

doi:10.1007/3-540-27331-x_4

Cited by 10 publications

(5 citation statements)

References 95 publications

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“…Occasionally, an internal collapse and emptying of the spore can be witnessed after a few days, after which the storage vesicles are ejected suddenly in one final load of cellular contents and nutrients into the attached hyphae (see Movie S4 † ). On several occasions, spore formation in asymbiotic hyphae, as reported by Dalpé et al , 44 was observed (see Movie S5 † ).…”

Section: Resultssupporting

confidence: 77%

“…40% of the Rhizophagus spore population to be trapped in the first half of the secondary trapping region. This trait of PDMS is actively utilised for cell trapping in microfluidics, mainly with yeast cells, 44–46 but has also been used to measure force exerted by fungal-like Oomycete hyphal tips. 47 Here, the two trapping mechanisms facilitate a reliable introduction of spores into and even distribution within the devices.…”

Section: Discussionmentioning

confidence: 99%

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AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

Richter,

Calonne-Salmon,

van der Heijden

et al. 2024

Lab Chip

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Section: Resultssupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

Richter,

Calonne-Salmon,

van der Heijden

et al. 2024

Lab Chip

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“…By contrast, those grown in the AM-DI substrate showed a higher density of arbuscules. The prevalence of vesicles (sites of lipid storage) over arbuscules (sites of resource exchange) indicates that the AM fungus had reached a more advanced stage of its life cycle [16]. As predicted, this tendency may be attributed to the fact that roots of AM-HDL plants likely had earlier colonization encounters with fungal propagules compared to roots of AM-DI plants which had a relatively more diffuse propagule distribution.…”

Section: Discussionmentioning

confidence: 65%

Identification of Constraining Experimental-Design Factors in Mycorrhizal Pot-Growth Studies

Audet

Charest

2010

Journal of Botany

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In the objective of testing the design of pot-growth experiments, we conducted two greenhouse studies of a “dwarf” sunflower cultivar and an arbuscular mycorrhizal (AM) fungus to determine how pot size and inoculum distribution affect plant growth and AM symbiosis. As predicted, large-potted plants developed a greater overall biomass and root colonization than small-potted ones which we attributed to the larger “rootable” volume. Furthermore, plants grown in a band of high density inoculum substrate showed a higher prevalence of fungal vesicles (sites of lipid storage) indicating a more advanced level of root colonization compared to those grown in a dispersed inoculum substrate; this likely being due to the higher frequency of interaction between roots and fungal propagules. In a second experiment, large-potted AM plants showed a greater tolerance to water deficit than non-AM control plants; however, this mycorrhizal effect was not detected among small-potted plants. We conclude that careful consideration should be made toward design parameters to limit result biases and ultimately facilitate comparison of findings between studies.

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“…If this becomes an issue, the pH could be stabilized with 2-[N-morpholino]ethanesulfonic acid (MES) buffer or SAP can be simply replaced with new polymer. The pH values recorded in SAP are known to support and promote germination (Dalpé et al 2005).…”

Section: Discussionmentioning

confidence: 99%

A simple and low-cost technique to initiate single-spore cultures of arbuscular mycorrhizal fungi using a superabsorbent polymer

Paré

Banchini²,

Hamel³

et al. 2022

Symbiosis

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Most species of arbuscular mycorrhizal fungi (AMF) are propagated with a host plant in a pot culture. However, the soil matrix makes it difficult to monitor the establishment and development of the symbiosis. In vitro culturing using Ri T-DNA transformed roots provides a clear medium and a sterile environment which offsets the constraints of the soil matrix. Nevertheless, the sterile conditions and the Ri T-DNA transformed roots provide very different growing conditions compared to a pot culture. Transparent soil based on superabsorbent polymer (SAP) has the potential of combining the advantages of current in vivo and in vitro culture methods without the constraints associated with either technique (opacity and sterility). Here we describe a SAP-based autotrophic culture as an alternative to current in vivo and in vitro culture methods. This system using two-compartment Petri dishes makes it easy to initiate single-spore cultures and to monitor fungal propagation. The SAP-based autotrophic system allowed the establishment of single-spore cultures of seven species (Diversispora varaderana, Funneliformis geosporus, Gigaspora rosea, Racocetra fulgida, Rhizophagus irregularis, R. intraradices and Sclerocystis sp.) from six genera and three families. Cultures were maintained over several months under non-sterile conditions. The Petri dishes avoid the problem of cross contamination and they can be stacked for space optimization. The grains of SAP colonized with new spores were used as inoculum to initiate new cultures in the SAP-based system. The SAP-based autotrophic culture method is a low-cost and low-tech approach, which makes the study of AMF much more accessible.

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Life Cycle of Glomus Species in Monoxenic Culture

Cited by 10 publications

References 95 publications

AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

Identification of Constraining Experimental-Design Factors in Mycorrhizal Pot-Growth Studies

A simple and low-cost technique to initiate single-spore cultures of arbuscular mycorrhizal fungi using a superabsorbent polymer

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