Identification of Constraining Experimental-Design Factors in Mycorrhizal Pot-Growth Studies

Audet, Patrick; Charest, Christiane

doi:10.1155/2010/718013

Cited by 14 publications

(13 citation statements)

References 17 publications

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“…Moreover, the grain biomass was reduced in comparison with when the AMF were allowed to access the second compartment but without a nutrient patch present. AMF may become more beneficial to the plant host with increasing pot size (Audet & Charest, 2010;Zangaro, Torezan, Rostirola, Souza, & Nogueira, 2015), and the current study provides evidence that this is due to the AMF being able to explore growth substrate beyond the reach of the plant roots. However, AMF have relatively high N demands and can act as a sink for patch-derived N (Herman, Firestone, Nuccio, & Hodge, 2012;Hodge & Fitter, 2010); hence, under low N conditions, as used in this study, AMF may become less mutualistic (Johnson, Wilson, Bowker, Wilson, & Miller, 2010;Puschel et al, 2016), perhaps due to AMF fulfilling their own nutritional requirements first, before passing nutrients to the host.…”

Section: Effects Of Direct Amf Access To Organic Nutrient Patches Omentioning

confidence: 62%

Aphids can acquire the nitrogen delivered to plants by arbuscular mycorrhizal fungi

et al. 2019

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Above‐ and below‐ground organisms can interact by altering the quality of shared host plants. Arbuscular mycorrhizal fungi (AMF) influence plant nutrient uptake, including nitrogen (N) acquisition. Under low N and phosphorus conditions, AMF delivery of N from organic sources not immediately available to the plant can have large impacts on plant N status, a limiting nutrient in the aphid diet. This study investigated the effect of AMF colonisation upon aphid number and determined the consequences of AMF directly accessing an organic nutrient patch that the plant cannot. We hypothesised that AMF colonisation of plants will increase plant and aphid N status, plant performance and aphid number, but only when the AMF had direct access to the added organic patch. Barley plants hosting the grain aphid Sitobion avenae were colonised by the AMF, Funneliformis mosseae, or no AMF. A two‐compartment microcosm was used to separate the plant roots from a 15N‐labelled organic patch in a second compartment. AMF‐colonised plants, but without access to the second compartment, were used to examine the effect of AMF colonisation on aphid number. In a separate treatment, and to determine whether AMF access to a plant inaccessible N source modified the effect of AMF colonisation on aphid number, AMF hyphae were permitted access to the second compartment containing an organic patch. As a control for AMF accessing a larger substrate volume, AMF were allowed access to a second compartment without an organic patch. When the AMF accessed the organic patch, more N from the patch was delivered to the plant resulting in a higher grain N concentration although plant growth was depressed. More N from the patch was also delivered to the aphids, but the N status of the aphid remained unchanged. Regardless of the level of access to the organic patch, AMF colonisation did not affect aphid number. Our data show that by accessing N sources not readily available to plants, AMF can indirectly deliver N to above‐ground organisms, a finding which has major implications for N‐transfer between higher trophic levels. A plain language summary is available for this article.

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Section: Effects Of Direct Amf Access To Organic Nutrient Patches Omentioning

confidence: 62%

Aphids can acquire the nitrogen delivered to plants by arbuscular mycorrhizal fungi

et al. 2019

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“…(). In this method, the tomato plant was kept for 50 days without the removal of the shoots and roots out of the ground and washed with distilled water for 24 h in “Long Ashton” nutrient solution (Audet & Charest ) [(K 2 SO 4 = 2.0, CaCl 2 anhydride = 4.0, MgSO 4 ·7H 2 O = 1.5, NaH 2 PO4·H 2 O = 1.5, MnSO 4 ·4H 2 O = 0.01, H 3 BO 3 = 0.05, NaCl = 0.09, EDTA‐Fe = 0.1) mM and (NH 4 NO 3 = 5.0, CuSO 4 ·5H 2 O = 1.0, ZnSO 4 ·7H 2 O = 1.0, Na 2 MoO 4 ·2H 2 O = 0.5) μM] at a temperature of 25°C.…”

Section: Methodsmentioning

confidence: 99%

Effects of arbuscular mycorrhizal fungal symbiosis for control of Egyptian broomrape (Orobanche aegyptiaca Pers.) in tomato (Lycopersicon esculentum L.) cultivation

Tadayyon

Zafarian

Fallah

et al. 2018

Weed Biology and Management

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The present study assessed the effect of arbuscular mycorrhizal fungi (AMF) on broomrape (Orobanche aegyptiaca Pers.) control to determine its effects on tomato (Lycopersicon esculentum L.) growth indices. This experiment was carried out using a randomized complete block design with four replications. The treatments included three strains of AMF (Glomus mosseae, Glomus intraradices and Glomuse hoe) at three levels (100, 200 and 300 kg ha −1 ). The control treatments (without mycorrhiza) were with and without broomrape (weed infest and weed free). The results showed that the use of G. intraradices and G. mosseae decreased broomrape seed germination, the number of nodules and the dry weight of the broomrape and increased root area and dry weight of the tomato plant when compared to G. hoe and the control treatments without broomrape. The tomato yield in G. intraradices 300 kg ha −1 treatment increased by 10 and 205% than weed free and weed infest treatments, respectively. Finally, G. intraradices and G. mosseae at, respectively, 200 and 300 kg ha −1 are recommended for land under tomato cultivation that has been contaminated with broomrape.

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“…The cultivation pot volume is an important characteristic to be taken into account to optimize the seedling production spaces in nurseries, and some studies show that the benefits obtained by plants via mycorrhizal symbiosis may differ with the size of cultivation pot (BÅÅTH, HAYMAN, 1994;CARDOSO et al, 2004;AUDET, CHAREST, 2010). However, these studies did not use native tropical woody species and were not focused on the nursery production.…”

Section: Introductionmentioning

confidence: 99%

INFLUENCE OF MYCORRHIZAS, ORGANIC SUBSTRATES AND CONTAINER VOLUMES ON THE GROWTH OF Heliocarpus popayanensis Kunth

Zangaro

Torezan

Rostirola

et al. 2015

CERNE

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This work assessed, under nursery conditions, the effect of arbuscular mycorrhizal fungi (AMF) inoculation on the initial growth of the woody species Heliocarpus popayanensis Kunth in containers of different sizes (nursery tubes of 50 or 250 cm3) containing composted cattle manure or organic Pinus spp bark compost diluted (0 to 100%, each 9%) with low fertility soil. Plants in cattle manure grew more than plants grown in pine bark manure independent of tube size. AMF were more efficient in improving plant growth in 250 cm3 tubes than in 50 cm3 tubes independent of the substrates. Mycorrhizal plants grown in 50 cm3 tubes showed less growth than non-mycorrhizal ones irrespective of the substrates. Nevertheless, this growth depression decreased with an increase of substrates dilution with low fertility soil. In the higher dilutions, growth depression did not occur and there was a positive response to AMF inoculation. In addition, only mycorrhizal plantlets showed some growth in low fertility soil as the sole substrate. These results indicated that AMF affect plantlet growth positively or negatively depending on the combination of substrates, fertility level, and container size.

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Identification of Constraining Experimental-Design Factors in Mycorrhizal Pot-Growth Studies

Cited by 14 publications

References 17 publications

Aphids can acquire the nitrogen delivered to plants by arbuscular mycorrhizal fungi

Aphids can acquire the nitrogen delivered to plants by arbuscular mycorrhizal fungi

Effects of arbuscular mycorrhizal fungal symbiosis for control of Egyptian broomrape (Orobanche aegyptiaca Pers.) in tomato (Lycopersicon esculentum L.) cultivation

INFLUENCE OF MYCORRHIZAS, ORGANIC SUBSTRATES AND CONTAINER VOLUMES ON THE GROWTH OF Heliocarpus popayanensis Kunth

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