Life Cycle, Bio-ecology and DNA Barcoding of mosquitoes Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse)

Anoopkumar, A. N.; Puthur, Sreedev; Varghese, P P; Rebello, Sharrel; Aneesh, Embalil Mathachan

doi:10.24321/0019.5138.201719

Cited by 13 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dengue is the most prevalent arbovirus disease worldwide, transmitted by the Aedes aegypti mosquito. It is estimated to have over 390 million cases per year and over 20,000 deaths on the global stage, mainly in tropical countries (Wu et al 2019 ; Anoopkumar et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Metarhizium robertsii protease and conidia production, response to heat stress and virulence against Aedes aegypti larvae

2021

View full text Add to dashboard Cite

Nutritional factors exert significant influence on the growth of entomopathogenic fungi, one of the main agents employed commercially in the biological control of arthropods. Thus, the objective of this work is to optimize the culture medium and solid fermentation time for production of proteases and conidia of Metarhizium robertsii ARSEF 2575 and to evaluate the interference of riboflavin and salts on virulence and resistance to abiotic stress factors. In the first step, nine groups were separated: negative control, positive control, and seven supplementation groups: ammonium nitrate, ammonium chloride, potassium nitrate, sodium nitrate, ammonium sulfate, ammonium phosphate, urea. Sodium nitrate showed significant difference in protease production at the time of 20 days of solid fermentation. Then, different concentrations of sodium nitrate and riboflavin as supplement were evaluated. Response surface methodology demonstrated that riboflavin and sodium nitrate influence proteolytic activity and conidia production, but without synergism. Supplementation of the medium with the optimal concentration of sodium nitrate and riboflavin did not interfere with the germination of conidia without exposure to abiotic stress, but did increase the thermotolerance of conidia. The presence of riboflavin and sodium nitrate at optimal concentrations in the culture medium did not alter fungal virulence with and without exposure to heat stress, varying according to the presence or absence of the supernatant during exposure, evidencing that resistance to heat exposure is multifactorial and dependent on intra- and extracellular factors. Moreover, the supplementation increased the larvicidal activity of the supernatant against Aedes aegypti.

show abstract

Section: Introductionmentioning

confidence: 99%