Licorice cream promotes full-thickness wound healing in Guinea pigs

Abstract

Impaired wound healing may cause economic and social problems and seriously reduce the quality of life. The uses of herbal medicines as new alternative treatments are under investigation. This study investigated the effect of Glycyrrhiza glabra L. extract on the full-thickness wound healing in Guinea Pig model. Eight male Guinea Pigs (700-800 g) were used to be creared a square full-thickness wound with 1.5 ×1.5 dimensions on the lumbodorsal area. The wounds were randomly divided into four groups: I; control, II; 1% phenytoin, III; 5% G. glabra and IV; 10 % G.glabra. On day 3, 5, 7, 9 and 12, wound size was measured for assessment of the percent of wound healing. In order to determine the wound healing activity, excisional biopsies were evaluated histopathologically on the 12th days of treatments. Acute inflammation, granulation tissue fibroblast maturation, collagen deposition, epidermal layer formation, neovascularization, keratin layer formation were evaluated according to the Abramov score method. Hydroalcoholic extract of G.glabra exhibited total phenolic and flavonoid contents of 114.1 ± 5.45 and 82.85 ± 6.38 mg, respectively. G.glabra creams (5% and 10% w/w)were significantly increased the epidermal formation, collagen deposition and neovascularization, and was decreased acute inflammation in comparison to the control group. Wound healing rate were increased in the G. glabra groups. G.glabra creams 10 % was more effective than 5% w/w. Our findings proved that 5% and 10% w/w G. glabra creams were effective in acute dermal wound healing. More studies with different doses of G. glabra extract are recommended.

The potential protective effect of licorice against age-related changes in the circumvallate papillae of albino rats

“…The tendency of licorice treatment to stimulate cell proliferative activity was previously observed in different in vitro (36,37) , animal (38,39) as well as clinical models (40) . In contrast, it's interesting to notice that licorice had been shown to cause apoptosis and cell cycle arrest in various cancer cell line types, exerting an anticancer impact (41,42) .…”

Sustainable Treatment of Oral Traumatic Ulcers with Licorice Containing Hydrogels: Integrating Computational Modeling, Quality by Design, Green Synthesis, and Molecular Biological Evaluation

“…Similarly, Oloumi et al examined the effect of Licorice root extract on dermal wounds in rats and the study revealed an increase in the number of fibroblasts, in addition to better re-epithelialization in Licorice-treated groups when compared to control groups [ 56 ]. Moreover, Hanafi et al also documented that Licorice creams significantly improved wound healing in Guinea pigs [ 57 ]. Furthermore, Najeeb and Al-Refai reported that Licorice root extract can shorten the healing time of induced oral mucosal wounds in rabbits [ 58 ].…”

Evaluation of Wound Healing Property of the Ethanolic Extract of Glycyrrhiza Glabra on Vero Cell Lines Using In Vitro Scratch Assay Test

“…G. Glabra, or Licorice, a native of south-east Europe and south-west Asia, was one of the widely used herbal medicine and a traditionally used flavoring/aromatic and calming plant that belongs to the family Papilionaceae/Fabaceae. [ 13 ] G. Glabra is a herbaceous perennial that may reach a height of 1 m. It has pinnate leaves that are 715 cm long and have 9-17 leaflets.…”

“…In their study, they found the neovascularization, epidermal formation, and collagen deposition were significantly improved by the application of G. Glabra extracts at different concentrations in the wounds when compared to the controls. [ 13 ]…”