Evaluation of Wound Healing Property of the Ethanolic Extract of Glycyrrhiza Glabra on Vero Cell Lines Using In Vitro Scratch Assay Test

Abstract

Introduction: The successful restoration of damaged tissue requires a complicated, dynamic process called wound healing, which is supported by a wide range of cellular activities. Natural materials generated from medicinal plants have been identified, and their therapeutic potential evaluation has resulted in the development of novel, affordable medicines that can be used to treat a variety of illnesses, including chronic wounds, with limited side effects. Aims and Objectives: This study aimed to assess the wound-healing property of Glycyrrhiza Glabra (Athimathuram) plant extracts by using an in vitro scratch assay test, as well as to evaluate their cellular toxicity. Materials and Methods: Using the Soxhlet device, ethanolic extraction of the plant material was done, and the cytotoxicity of the extract on the Vero cell line was examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. One of the most often utilized mammalian in vitro cell lines in research was Vero cells. To assess the wound healing properties of G. glabra plant extract, an in vitro scratch assay was used, and their potential mechanisms of action were examined. Results: Even at higher concentrations, the MTT assay showed that G. Glabra plant extracts had no cytotoxic effects on the cells. In vitro scratch assay showed that the healing process of the cell line was increased by 23.33% when compared with the controlled cell lines. Conclusion: Our research demonstrated that G. glabra has in vitro wound healing capabilities. As a result, G. glabra can be suggested as a possible source of compounds that treat wounds.

Phytochemical Assessment, Antioxidant Activity, and in vitro Wound Healing Potential of Polygonum minus Huds

“…The results indicate that the P. minus aqueous extract did not induce any cytotoxic effects on HaCaT cells at all tested concentrations, with the percentage of cell viability consistently exceeding 80%. In line with the findings of Roy et al (2023) and Che Zain et al (2020), extracts are considered non-toxic when cell viability remains above 80%. Previous research supports these findings, demonstrating the non-toxic nature of P. minus aqueous extract on both normal endothelial (EA.hy926) and cancer (HCT116, HT29, and HeLa) cells (Christapher et al, 2016).…”

Exploring Skin Wound Healing Models and the Impact of Natural Lipids on the Healing Process

“…This process induces cellular trauma, initiating a cascade of events, including proliferation, protein production, and changes in viability, migration, gene expression, and differentiation, and opens an area that must be covered and closed. Single-cell-type models are frequently employed to test agents that may enhance skin cell migration and re-epithelialization [44,45].…”

Infuse herbal oils: a comparative study of wheat germ and tomato seed oils

“…Migration in the cavity of the cell monolayer was photographed and then using a computer program (ImageJ) the results were calculated as percent migration compared to the control group. The results were reported as the mean of three replicates (Liang et al, 2007;Roy et al, 2023).…”