Liberation of ATP secondary to hemolysis is not mutually exclusive of regulated export

Kirby, Brett S.; Schwarzbaum, Pablo J.; Lazarowski, Eduardo R.; Dinenno, Frank A.; McMahon, Timothy J.

doi:10.1182/blood-2014-11-609610

Cited by 14 publications

(16 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 In the course of the study, while attempting to reproduce previously published findings, we identified a number of methodological pitfalls (the major ones are discussed in the supplemental Methods available on Blood Web site), which likely precluded a proper evaluation of ATP release and also the contribution of hemolysis in the earlier studies 2-10 cited by Kirby et al We agree that "liberation of ATP secondary to hemolysis is not mutually exclusive of regulated export." 11 Our point is that without the controls described in our paper, a confounding effect of hemolysis cannot be excluded.…”

Section: Responsementioning

confidence: 77%

Response: Hemolysis is a primary and physiologically relevant ATP release mechanism in human erythrocytes

Sikora

Orlov

Furuya

et al. 2015

Blood

View full text Add to dashboard Cite

Section: Responsementioning

confidence: 77%

Response: Hemolysis is a primary and physiologically relevant ATP release mechanism in human erythrocytes

Sikora

Orlov

Furuya

et al. 2015

Blood

View full text Add to dashboard Cite

“…This methodological issue has been 585 deeply considered by various researchers [see e.g. [123][124][125]. 586 Nevertheless, the exercise-induced increase of ATP release from 587 erythrocytes is considered an attractive candidate for serving as 588 explanation of the enhancement of the coronary blood flow during 589 exercise [see e.g.…”

mentioning

confidence: 99%

Coronary and muscle blood flow during physical exercise in humans; heterogenic alliance

Zoladz

Majerczak

Duda

et al. 2015

Pharmacological Reports

View full text Add to dashboard Cite

In this review, we present the relation between power generation capabilities and pulmonary oxygen uptake during incremental cycling exercise in humans and the effect of exercise intensity on the oxygen cost of work. We also discuss the importance of oxygen delivery to the working muscles as a factor determining maximal oxygen uptake in humans. Subsequently, we outline the importance of coronary blood flow, myocardial oxygen uptake and myocardial metabolic stability for exercise tolerance. Finally, we describe mechanisms of endothelium-dependent regulation of coronary and skeletal muscle blood flow, dysregulation of which may impair exercise capacity and increase the cardiovascular risk of exercise.

show abstract

“…24 However, there is some question as to whether the use of 1-to 14-day-old cells in work investigating hemolysis has obfuscated other mechanisms of ATP release. 25 The main goal of this work was to assess whether a large spike in ATP mechanotransduction occurs downstream of a short constriction, using blood drawn from a larger cohort of participants. We show that RBCs drawn from n = 15 different participants all exhibited shearinduced ATP release, consistent with previous results by numerous researchers who employed a variety of different mechanical stimuli.…”

Section: And Edwards Et Al 7 Corroboratedmentioning

confidence: 99%

“…Furthermore, doubt has been cast on the mechanism for mechanotransduction, with some evidence pointing toward hemolysis as the primary mechanism for ATP release . However, there is some question as to whether the use of 1‐ to 14‐day‐old cells in work investigating hemolysis has obfuscated other mechanisms of ATP release …”

Section: Introductionmentioning

confidence: 99%

A spike in mechanotransductive adenosine triphosphate release from red blood cells in microfluidic constrictions only occurs with rare donors

Mancuso

Ristenpart

2018

Microcirculation

View full text Add to dashboard Cite

ObjectiveWan et al (Proc Natl Acad Sci USA, 105, 2008, 16432) demonstrated that RBCs rapidly and transiently release a spike of 300% more ATP shortly downstream from a short microfluidic constriction where the cells experience a sudden increase in shear stress. More recent work by Cinar et al (Proc Natl Acad Sci USA, 112, 2015, 11783), however, yielded no evidence for a similar spike in ATP release downstream of the constriction. Our aim was to determine whether a transient spike in mechanotransduction is the typical response of RBCs to the sudden onset of increased shear.MethodsWe investigate ATP release downstream of a microfluidic constriction for 15 participants using a luciferase‐based photoluminescent assay.ResultsWhile we observe mechanotransductive ATP release from blood drawn from all donors, we find evidence of a spike in ATP concentration after the microfluidic constriction for only 2 of 15 participants. No clear trends in ATP release are found with respect to the magnitude of the applied shear stress, or to the gender, age, or physical activity (Baecke) index of the donor.ConclusionsIn aggregate, all data acquired to date suggest that a spike in mechanotransductive ATP due to a suddenly applied increase in shear stress occurs in blood drawn from only 14% of the population.

show abstract

Liberation of ATP secondary to hemolysis is not mutually exclusive of regulated export

Cited by 14 publications

References 12 publications

Response: Hemolysis is a primary and physiologically relevant ATP release mechanism in human erythrocytes

Response: Hemolysis is a primary and physiologically relevant ATP release mechanism in human erythrocytes

Coronary and muscle blood flow during physical exercise in humans; heterogenic alliance

A spike in mechanotransductive adenosine triphosphate release from red blood cells in microfluidic constrictions only occurs with rare donors

Contact Info

Product

Resources

About