Lgl1 Is Required for Olfaction and Development of Olfactory Bulb in Mice

Abstract

Lethal giant larvae 1 (Lgl1) was initially identified as a tumor suppressor in Drosophila and functioned as a key regulator of epithelial polarity and asymmetric cell division. In this study, we generated Lgl1 conditional knockout mice mediated by Pax2-Cre, which is expressed in olfactory bulb (OB). Next, we examined the effects of Lgl1 loss in the OB. First, we determined the expression patterns of Lgl1 in the neurogenic regions of the embryonic dorsal region of the LGE (dLGE) and postnatal OB. Furthermore, the Lgl1 conditional mutants exhibited abnormal morphological characteristics of the OB. Our behavioral analysis exhibited greatly impaired olfaction in Lgl1 mutant mice. To elucidate the possible mechanisms of impaired olfaction in Lgl1 mutant mice, we investigated the development of the OB. Interestingly, reduced thickness of the MCL and decreased density of mitral cells (MCs) were observed in Lgl1 mutant mice. Additionally, we observed a dramatic loss in SP8+ interneurons (e.g. calretinin and GABAergic/non-dopaminergic interneurons) in the GL of the OB. Our results demonstrate that Lgl1 is required for the development of the OB and the deletion of Lgl1 results in impaired olfaction in mice.

3d virtual histology of neuronal tissue by propagation-based x-ray phase-contrast tomography

“…Within the virtual slice through the olfactory bulb, distinct layers can be recognized, namely the glomerular layer, containing the spherical glomeruli, the external plexiform layer, the mitral cell layer, internal plexiform layer and granular layer [101]. In the results from the brain stem, different features as the air-filled blood vessels as well as thick fiber bundles, which are visible as round structures of lower electron density, can be recognized.…”

Role of LGL1 in cerebellar primordium of embryonic mice

“…Samples were blocked with 5% (vol/vol) normal goat serum in PBS and incubated with primary antibodies including rabbit anti-Hugl-1 (Santa Cruz, Heidelberg, Germany, 1:50). This antibody was characterized in our previous studies [6][7][8], employed to detect the mouse homologue Lgl-1. Mouse anti-Nestin (Chemicon, Temecula, CA, USA, 1:200), rabbit anti-Cdc42 (Proteintech, Wuhan, China, 1:500), mouse anti-β-catenin (Proteintech, 1:300).…”

3d virtual histology of neuronal tissue by propagation-based x-ray phase-contrast tomography

“…As for the structures considered before, the anatomical features expected from histological studies are clearly resolved at cellular detail. Within the virtual slice through the olfactory bulb, distinct layers can be recognized, namely the glomerular layer, containing the spherical glomeruli, the external plexiform layer, the mitral cell layer, internal plexiform layer and granular layer [101]. In the results from the brain stem, different features as the air-filled blood vessels as well as thick fiber bundles, which are visible as round structures of lower electron density, can be recognized.…”