Lewis X and Sialyl Lewis X Glycosphingolipids.

Cooling, Laura; Zhang, Desheng; Koerner, Theodore A.W.; 康夫, 鈴木

doi:10.4052/tigg.9.191

Cited by 12 publications

(16 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CD15s carbohydrate itself is present on >10 7 molecules per cell. Its underlying carbohydrate scaffolding varies in length and branching, and CD15s modifies many different proteins and sphingolipids (26,27). Significant differences in biologic function can occur based on variations in this scaffolding, and anti-CD15s MABs may not be capable of fully blocking binding to all of these moieties.…”

Section: Discussionmentioning

confidence: 99%

Leukocyte infection by the granulocytic ehrlichiosis agent is linked to expression of a selectin ligand

et al. 1999

View full text Add to dashboard Cite

Human granulocytic ehrlichiosis (HGE) is a newly recognized tickborne infection of humans (1-3). HGE is a febrile illness characterized by reductions in blood leukocyte and platelet counts and the presence of colonies of infecting organisms (morulae) proliferating within cytoplasmic vacuoles of circulating neutrophils. No other organism is known to replicate within human granulocytes. Our laboratory recently isolated and propagated the causative agent of this disease using the human promyelocytic leukemia cell line HL60 (4). In addition, we found that the agent can grow in primary bone marrow granulocytic and monocytic precursors, blood neutrophils, and nonactivated monocytes (5).The clinical and in vitro tropism of this obligate intracellular parasite for myelomonocytic cells suggested that specific cell-surface molecules are required for adhesion, cell signaling, and entry into a permissive intracellular compartment. Of interest, sialyl Lewis x ([NeuAcα(2− 3)GalΒ1−4(Fucα1-3)GlcNAc, or CD15s]), which serves as a ligand to initially tether leukocytes to endothelial cell Eselectin (6, 7), is richly expressed on both HL60 cells and on those bone marrow and blood cells permissive for the HGE agent (8-11), where it modifies a variety of lipid and protein molecules. We hypothesized that cell-surface sialylated Lewis x might be important for infection by HGE. Methods HGE infection, cultivation, and antibody blocking studies.A cell-free bacterial inoculum of HGE was prepared from fully infected HL60 cells, propagated as described (4). The infected cells, at a density of 10 6 per ml, were passed through a 25-gauge needle three times, and cellular debris were removed by centrifugation at 700 g for 5 min. The supernatant was centrifuged at 1,236 g for 5 min, and the resultant bacterial pellet was resuspended in 10 µl supernatant per ml of the original culture. Counting in a Petroff-Hausser chamber revealed ∼10 7 organisms per 10 µl. Infections were carried out by coincubating 10 µl of this cell-free HGE preparation with 10 6 cells for a multiplicity of infection of ∼10 organisms per cell used in all experiments. For studies to determine whether bacterial binding requires calcium, both HL60 cells and the bacterial inoculum were separately incubated in HBSS with or without calcium for 20-70 min before coincubation, as described later here. For blocking studies, 10 µg (or the quantity specified) of the monoclonal antibody (MAB) tested (or control diluent or murine IgM alone) was added to 1 ml of 10 6 HL60 cells (or bone marrow progenitors, peripheral blood monocytes, or granulocytes, prepared as described; ref. 5) in medium and incubated at room temperature for 1 h. Blocking studies were performed six times with similar results. As noted, antibody blocking studies were also performed at 4°C or 37°C. Cells were centrifuged at 200 g for 5 min, 900 µl of supernatant saved, the cells resuspended in the 100 µl of medium remaining, and 10 µl of the HGE bacteria added and then coincubated for 15 min at room temperature. The cells w...

show abstract

Section: Discussionmentioning

confidence: 99%

Leukocyte infection by the granulocytic ehrlichiosis agent is linked to expression of a selectin ligand

et al. 1999

View full text Add to dashboard Cite

show abstract

“…Chan and Watt, 1 Haltiwanger, 2 Sanchez-Madrids, 3 Cooling et al, 4 Mazo and von Adrian 5 Chan and Watt, 1 Haltiwanger, 2 Gonzalez-Amaro and Sanchez-Madrid, 3 Cooling et al, 4 Mazo and von Adrian 5 Notch…”

Section: Lementioning

confidence: 99%

“…1,2 Lewis X (Le X ) and sialylated Lewis X (sLe X ; Table 1) are two blood group-related antigens that serve as ligands for selectins (L-, P-, and E-selectin)-a family of vascular addressins that mediate white blood cell (WBC) adhesion to endothelium. 3,4 Among hematopoietic cells, Le X and sLe X are expressed on CD34 + progenitor cells, early lymphoid progenitors, monocytes, and granulocytes, where they facilitate homing to marrow sinusoids, lymph nodes, and sites of inflammation. 1,3,5 Fucosylation also plays a central role in the Notch signaling pathway, an evolutionarily conserved system regulating cell differentiation.…”

mentioning

confidence: 99%

Delayed platelet engraftment in group O patients after autologous progenitor cell transplantation

et al. 2005

View full text Add to dashboard Cite

show abstract

“…In human and animal tissue, ganglioside expression is often tissue‐ and species‐specific and may alter with cellular differentiation and malignancy. Not surprisingly, gangliosides have been shown to play a role in cell growth, 3 adhesion, 3–6 differentiation, 4,7,8 neoplasia, 5–6 and transmembrane signaling 3,4,8,9 . In infectious disease, gangliosides serve as specific receptors for several bacteria, viruses, and toxins 10–14 .…”

Section: Structure and Mobility Of Platelet Gangliosides2424mentioning

confidence: 99%

Human platelets express gangliosides with LKEactivity and ABH blood group activity

2001

View full text Add to dashboard Cite

show abstract

Lewis X and Sialyl Lewis X Glycosphingolipids.

Abstract: Lewis X (Lex) and sialyl Lewis X (sLex) are carbohydrate blood group antigens important in cell adhesion. On cell membranes, Lex and sLexmay be expressed on glycoproteins or

Cited by 12 publications

References 78 publications

Leukocyte infection by the granulocytic ehrlichiosis agent is linked to expression of a selectin ligand

Leukocyte infection by the granulocytic ehrlichiosis agent is linked to expression of a selectin ligand

Delayed platelet engraftment in group O patients after autologous progenitor cell transplantation

Human platelets express gangliosides with LKEactivity and ABH blood group activity

Contact Info

Product

Resources

About