Human platelets express gangliosides with LKEactivity and ABH blood group activity

Abstract

In addition to sialyl-iI and sialyl-Le(x) gangliosides, group A platelets express gangliosides with LKE activity and group A activity. Like RBCs, group A-active gangliosides may act as alloantigens and autoantigens to naturally occurring isohemagglutinins.

Impact of ABO incompatibility on engraftment and transfusion requirement after unrelated cord blood transplantation: a single institute experience in Japan

“…Badros et al 16 also showed that major/ bidirectional ABO incompatibility led to delayed platelet engraftment and an increased requirement for platelet transfusion in patients who underwent PBSCT using a nonmyeloablative conditioning regimen. Although ABH antigens are also expressed on platelets, 17,18 most of the previous studies did not show an association between major/bidirectional ABO incompatibility and delayed platelet engraftment or increased platelet transfusion requirement after SCT. The reason for the delayed platelet engraftment in major/bidirectional ABO-incompatible CBT could not be clearly explained.…”

Determinants of ABH expression on human blood platelets

“…GSLs were detected by chemical staining with diphenylamine reagent or by HPTLCimmunostaining with blood group-specific MoAbs as described. 3,25 All samples were immunostained in duplicate. All samples staining negative for either A, B, or Lewis antigens were repeated at twice the initial GSL concentration.…”

“…Membranes were immunostained for GPIIb (CD41b, AMAC, Westbrook, ME) and ABH using the ABC streptavidin system (Vector Laboratories) described for HPTLC-immunostaining. 3,24,25 Gels were retained and stained with Coomassie Brilliant Blue. All Western blot-negative samples were repeated at 80 g/well.…”

“…2,3 In the neutral GSL fraction, platelets express 2 major A-GSL bands, A-6 and A-7/8 ( Figure 3D). These 2 bands represent up to 4 separate GSL species, including endogenous type 2 chain GSLs (A-6-2, A-8-2) from megakaryocytes, as well as adsorbed type 1 chain ABH (A-6-1) and Lewis antigens (ALe b , A-7-1) from plasma.…”

“…[2][3][4][5][6][7][8] Clinically, transfusion of ABHincompatible platelets can be associated with decreased recovery, [8][9][10][11] shortened survival, 10 and an increased incidence and onset of HLA-immune refractoriness. [11][12][13] ABH incompatibility can lead to positive platelet crossmatches, 14 acting synergistically with HLA incompatibility to further decrease posttransfusion recovery and survival.…”