Levosimendan Attenuates Reperfusion Injury in an Isolated Perfused Rat Heart Model

Öztürk, Tülün; Gök, Şule; Neşe, Nalan

doi:10.1053/j.jvca.2009.08.003

Cited by 9 publications

(6 citation statements)

References 30 publications

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“…Perfusions containing LS, based on its myocardial protective effect, can inhibit apoptosis of myocardial cells and upregulate the expression of bcl-2 gene, also resulting in inhibition of apoptosis. 14) This suggests that the lung-protective effect of LS is mediated by inhibition of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Levosimendan Reduces Lung Injury in a Canine Model of Cardiopulmonary Bypass

Feng

Liu²,

Chen

et al. 2016

Korean Circ J

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Background and ObjectivesTo explore the lung-protective effect of levosimendan (LS) during cardiopulmonary bypass in a canine model by determining the wet/dry weight (W/D) ratio of lung tissue, malonaldehyde (MDA) and superoxide dismutase (SOD) concentrations, and performing a histological evaluation.Materials and MethodsThirty-two canines were divided randomly into four groups and underwent a routine aortic cross-clamping cardiopulmonary bypass procedure for 1 h, followed by recovery for 2 h. Animals were handled as follows: group C (means control group), no special treatment after aortic cross clamping; group P (means pulmonary artery perfusion group), pulmonary artery perfusion with cold oxygenated blood after aortic cross clamping; group LSIV (means intravenous injection of LS group), intravenous injection of LS (65 µg/kg) before thoracotomy, and the rest of the procedure was identical to the control group; group LPS (means pulmonary perfusion with LS group), pulmonary perfusion with cold oxygenated blood combined with LS (65 µg/kg) after aortic cross clamping. Lung tissues were removed and subjected to evaluation of pathological alterations, W/D ratio and MDA and SOD concentrations.ResultsIn group C, the W/D ratio and MDA concentration were higher, while the SOD concentrations were lower (p<0.05). Compared with groups P and LSIV, the MDA concentration was lower in group LPS, while that of SOD was higher (p<0.05); Light and electron microscopy indicated that LS intervention reduced impairment of lung tissues.ConclusionOur findings suggest that LS plays an important role in protecting lung tissues.

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Section: Discussionmentioning

confidence: 99%

Levosimendan Reduces Lung Injury in a Canine Model of Cardiopulmonary Bypass

Feng

Liu²,

Chen

et al. 2016

Korean Circ J

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“…Moreover, levosimendan induces preconditioning and decreases myocardial infarct size in an isolated perfused rat heart model of acute ischemia–reperfusion injury (Ozturk et al, 2010). This is the first study to evaluate the effects of levosimendan on cardiomyocyte apoptosis in an in vivo experimental model of global, cardioplegic, myocardial ischemia that closely resembles human cardiac surgery.…”

Section: Discussionmentioning

confidence: 99%

Intracoronary Levosimendan during Ischemia Prevents Myocardial Apoptosis

Malmberg¹,

Vähäsilta²,

Saraste³

et al. 2012

Front. Physio.

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Background: Levosimendan is a calcium sensitizer that has been shown to prevent myocardial contractile depression in patients post cardiac surgery. This drug exhibits an anti-apoptotic property; however, the underlying mechanism remains elusive. In this report, we characterized the myocardial protective of levosimendan in preventing cardiomyocyte apoptosis and post-operative stunning in an experimental ischemia–reperfusion model. Methods: Three groups of pigs (n = 8 per group) were subjected to 40 min of global, cardioplegic ischemia followed by 240 min of reperfusion. Levosimendan (65 μg/kg body weight) was given to pigs by intravenous infusion (L-IV) before ischemia or intracoronary administration during ischemia (L-IC). The Control group did not receive any levosimendan. Echocardiography was used to monitor cardiac function in all groups. Apoptosis levels were assessed from the left ventricle using the terminal transferase mediated dUTP nick end labeling (TUNEL) assay and immunocytochemical detection of Caspase-3. Results: Pigs after ischemia–reperfusion had a much higher TUNEL%, suggesting that our treatment protocol was effective. Levels of apoptosis were significantly increased in Control pigs that did not receive any levosimendan (0.062 ± 0.044%) relative to those received levosimendan either before (0.02 ± 0.017%, p = 0.03) or during (0.02 ± 0.017%, p = 0.03) the ischemia phase. Longitudinal left ventricular contraction in pigs that received levosimendan before ischemia (0.75 ± 0.12 mm) was significantly higher than those received levosimendan during ischemia (0.53 ± 0.11 mm, p = 0.003) or Control pigs (0.54 ± 0.11 mm, p = 0.01). Conclusion: Our results suggested that pigs received levosimendan displayed a markedly improved cell survival post I–R. The effect on cardiac contractility was only significant in our perfusion heart model when levosimendan was delivered intravenously before ischemia.

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“…Similar findings were reported by Maytin and Colucci [23] who showed in their in vitro study that levosimendan, even at very low concentrations, protected cardiomyocytes from H 2 O 2 -induced apoptosis by activating mitochondrial ATP-dependent K + channels. Öztürk et al [24] also found that levosimendan can induce B-cell lymphoma (Bcl-2) expression and reduce the number of TUNELpositive cardiomyocytes in isolated rat hearts. They also showed that the myocardial infarct size was reduced compared with the controls.…”

Section: Discussionmentioning

confidence: 96%

Levosimendan attenuates ischemia/reperfusion injury of the heart better than ischemic preconditioning during moderate hypothermia

Yapıcı¹

2014

Turk Gogus Kalp Dama

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Levosimendan Attenuates Reperfusion Injury in an Isolated Perfused Rat Heart Model

Cited by 9 publications

References 30 publications

Levosimendan Reduces Lung Injury in a Canine Model of Cardiopulmonary Bypass

Levosimendan Reduces Lung Injury in a Canine Model of Cardiopulmonary Bypass

Intracoronary Levosimendan during Ischemia Prevents Myocardial Apoptosis

Levosimendan attenuates ischemia/reperfusion injury of the heart better than ischemic preconditioning during moderate hypothermia

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