Levitation, holding, and rotation of cells within traps made by high-frequency fields

Fuhr, G.; Arnold, W.M.; Hagedorn, Rolf; Müller, Torsten; Benecke, W.; Wagner, B.; Zimmermann, U.

doi:10.1016/0005-2736(92)90028-k

Cited by 124 publications

(69 citation statements)

References 29 publications

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“…Consistent with earlier observations for endothelial cells, [47] NIH 3T3 cells patterned on straight 30 lm wide lines migrate randomly in both left and right directions and, as a result, show no significant displacement over comparable time periods. Compared to existing methods for positioning cells, [48][49][50][51] one-way microarrays can be used to control continuously and simultaneously the migratory direction and speed of an arbitrary number of cells over long distances without external fields or stimulus gradients. The design principles for creating these microarrays can be implemented on tissue-culture dishes and other biocompatible substrates using a variety of micropatterning techniques [52][53][54][55][56][57][58][59][60] that are now readily accessible in many laboratories.…”

mentioning

confidence: 99%

Retracted: Guiding Cell Migration Using One‐Way Micropattern Arrays

Kumar

2007

Advanced Materials

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A single NIH 3T3 fibroblast on a teardrop island, surrounded by a cell‐resistant background, extends lamellipodia from both sharp and blunt ends. However, the staggered arrangement of the islands and preferential extension of lamellipodia parallel to the cell body only permit attachment of lamellipodia extended from the blunt end, resulting in exclusive counterclockwise migration (the figure is ca. 200 μm wide).

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mentioning

confidence: 99%

Retracted: Guiding Cell Migration Using One‐Way Micropattern Arrays

Kumar

2007

Advanced Materials

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“…If the field is non-uniform, the particle will experience a resultant translational force (DEP: dielectrophoresis) [3][4][5][6][7][8]. Alternatively, if the field is rotating, the interaction between the field and the induced dipole will make the particle rotate (ROT: electrorotation) [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Role of peroxide in AC electrical field exposure effects on Friend murine erythroleukemia cells during dielectrophoretic manipulations

Wang

Yang

Gascoyne

1999

Biochimica Et Biophysica Acta (BBA) - General Subjects

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The effects of AC field exposure on the viability and proliferation of mammalian cells under conditions appropriate for their dielectrophoretic manipulation and sorting were investigated using DS19 murine erythroleukemia cells as a model system. The frequency range 100 Hz-10 MHz and medium conductivities of 10 mS/m, 30 mS/m and 56 mS/m were studied for fields generated by applying signals of up to 7V peak to peak (p-p) to a parallel electrode array having equal electrode widths and gaps of 100 μm. Between 1 kHz and 10 MHz, cell viability after up to 40 min of field exposure was found to be above 95% and cells were able to proliferate. However, cell growth lag phase was extended with decreasing field frequency and with increasing voltage, medium conductivity and exposure duration. Modified growth behavior was not passed on to the next cell passage, indicating that field exposure did not cause permanent alterations in cell proliferation characteristics. Cell membrane potentials induced by field exposure were calculated and shown to be well below values typically associated with cell damage. Furthermore, medium treated by field exposure and then added to untreated cells produced the same modifications of growth as exposing cells directly, and these modifications occurred only when the electrode polarization voltage exceeded a threshold of ~0.4 V p-p. These findings suggested that electrochemical products generated during field exposure were responsible for the changes in cell growth. Finally, it was found that hydrogen peroxide was produced when sugar-containing media were exposed to fields and that normal cell growth could be restored by addition of catalase to the medium, whether or not field exposure occurred in the presence of cells. These results show that AC fields typically used for dielectrophoretic manipulation and sorting of cells do not damage DS19 cells and that cell alterations arising from electrochemical effects can be completely mitigated.

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“…Cell sedimentation occurs when the gravitation force is higher than the buoyancy force. Cells, which are trapped in the nDEP octupole cage, additionally experience the nDEP field force in vertical direction avoiding cell sedimentation during long-term cultivations [31][32][33].…”

Section: Cell Separation and Trapping With Ndepmentioning

confidence: 99%

An Inert Continuous Microreactor for the Isolation and Analysis of a Single Microbial Cell

et al. 2015

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Studying biological phenomena of individual cells is enabled by matching the scales of microbes and cultivation devices. We present a versatile, chemically inert microfluidic lab-on-a-chip (LOC) device for biological and chemical analyses of isolated microorganisms. It is based on the Envirostat concept and guarantees constant environmental conditions. A new manufacturing process for direct fusion bonding chips with functional microelectrodes for selective and gentle cell manipulation via negative dielectrophoresis (nDEP) was generated. The resulting LOC system offered a defined surface chemistry and exceptional operational stability, maintaining its structural integrity even after harsh chemical treatment. The microelectrode structures remained fully functional after thermal bonding and were proven to be efficient for single-cell trapping via nDEP. The microfluidic network consisted solely of glass, which led to enhanced chip reusability and minimized interaction of the material with chemical and biological compounds. We validated the LOC for single-cell studies with the amino acid secreting bacterium Corynebacterium glutamicum. Intracellular L-lysine production dynamics of individual bacteria were monitored based on a genetically encoded fluorescent nanosensor. The results demonstrate the applicability of the presented LOC for pioneering chemical and biological studies, where robustness and chemically inert surfaces are crucial parameters for approaching fundamental biological questions at a single-cell level.

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Levitation, holding, and rotation of cells within traps made by high-frequency fields

Cited by 124 publications

References 29 publications

Retracted: Guiding Cell Migration Using One‐Way Micropattern Arrays

Retracted: Guiding Cell Migration Using One‐Way Micropattern Arrays

Role of peroxide in AC electrical field exposure effects on Friend murine erythroleukemia cells during dielectrophoretic manipulations

An Inert Continuous Microreactor for the Isolation and Analysis of a Single Microbial Cell

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