Retracted: Guiding Cell Migration Using One‐Way Micropattern Arrays

Kumar, Girish; Ho, Chia‐Chi; Co, Carlos C.

doi:10.1002/adma.200601629

Cited by 93 publications

(101 citation statements)

References 57 publications

(44 reference statements)

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“…In the study of chemical and topographical pattern on this surface, we have already known that cells respond differently to variations in surface chemistry and can specifically distinguish between proteins or even peptides of a few amino acids3. Directional control of cell movement along preset paths can be realized on the microarrays of asymmetric cell-adhesive islands4. In recent years, it has become increasingly evident that the cellular response to environmental signals goes far beyond the ability of the cell to surface chemistry and topography, and thus emphasis has been focused on the mechanics of biointerface, especially on matrix stiffness567.…”

mentioning

confidence: 99%

Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading

Han

Zhao

2014

Sci Rep

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To describe detailed behaviour of cell spreading under the influence of substrate stiffness, A549 cells cultured on the surfaces of polydimethylsiloxane (PDMS) and polyacrylamide (PAAm) with bulk rigidities ranging from 0.1 kPa to 40 kPa were in situ observed. The spreading behaviour of cells on PAAm presented a positive correlation between spreading speed and substrate stiffness. After computing the deformations of PAAm gels and collagen, the bulk stiffness of PAAm, rather than matrix tethering, determined the cell behaviour. On the other hand, spreading behaviour of the cells was unaffected by varying the bulk stiffness of PDMS. Based on simulation analyses, the elasticity of silica-like layer induced by UV radiation on PDMS surface dominated cell-substrate interaction, rather than the bulk stiffness of the material, indicating that it is the interfacial stiffness that mainly guided the cell spreading. And then the kinetics of cell spreading was for the first time modeled based on absolute rate theory.

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mentioning

confidence: 99%

Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading

Han

Zhao

2014

Sci Rep

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“…Similarly, cells grown on narrow stripes align themselves along the orientation of the stripes. Substrate geometry also affects cell polarity and provides guidance for cell expansion and migration direction (Jiang et al, 2005; Kumar et al, 2007). Although these results provide the end-point of cell morphology under a mechanical constraint, only recently the role of internal mechanical forces in cell remodeling has been explored, namely, the process leading to the end point (DuFort et al, 2011; Grashoff et al, 2010; Maruthamuthu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Intracellular forces during guided cell growth on micropatterns using FRET measurement

Suffoletto

Meng

et al. 2015

Journal of Biomechanics

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Interaction of cells with extracellular matrix (ECM) regulates cell shape, differentiation and polarity. This effect has been widely observed in cells grown on substrates with various patterned features, stiffness and surface chemistry. It has been postulated that mechanical confinement of cells by the substrate causes a redistribution of tension in cytoskeletal proteins resulting in cytoskeletal reorganization through force sensitive pathways. However, the mechanisms for force transduction during reorganization remain unclear. In this study, using FRET based force sensors we have measured tension in an actin cross-linking protein, α-actinin, and followed reorganization of actin cytoskeleton in real time in HEK cells grown on patterned substrates. We show that the patterned substrates cause a redistribution of tension in α-actinin that coincides with cytoskeleton reorganization. Higher tension was observed in portions of cells where they form bridges across inhibited regions of the patterned substrates; the attachment to the substrate is found to release tension. Real time measurements of α-actinin tension and F-actin arrangement show that an increase in tension coincides with formation of F-actin bundles at the cell periphery during cell-spreading across inhibited regions, suggesting that mechanical forces stimulate cytoskeleton enhancement. Rho-ROCK inhibitor (Y27632) causes reduction in actinin tension followed by retraction of bridged regions. Our results demonstrate that changes in cell shape and expansion over patterned surfaces is a force sensitive process that requires actomyosin contractile force involving Rho-ROCK pathway.

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“…The direction of the movement depended on the presence of an adjacent island along the axis of the elongated cell body. [196] Cells did not always move in the direction of the blunt end as would be expected from cell polarization on isolated tear-drop shape islands (as shown in Figure 13d) where wider end promotes lamellipodial protrusion, while sharp end promotes formation of the tail. The same group has designed micropatterns consisting of 15tear-drop shapes joined head-to-tail to form a circular track (Figure 14c, ii).…”

Section: Probing Cytoskeletal Structures Cell Mechanics and Cell mentioning

confidence: 89%

“…Kumar et al used patterns consisting of four tear-drop shapes separated by small gaps (~3 µm) and arranged at 90° angles to form square-shaped tracks that guided cell motions unidirectionally (Figure 14c, i). [196,197] Two variations of this pattern with different alignment of the blunt and sharp ends of the tear-drop shapes were shown to guide NIH 3T3 fibroblast cell motions in opposite directions. The direction of the movement depended on the presence of an adjacent island along the axis of the elongated cell body.…”

Section: Probing Cytoskeletal Structures Cell Mechanics and Cell mentioning

confidence: 99%

See 1 more Smart Citation

Microfabricated Systems and Assays for Studying the Cytoskeletal Organization, Micromechanics, and Motility Patterns of Cancerous Cells

Huda

Pilans

Makurath

et al. 2014

Adv Materials Inter

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Cell motions are driven by coordinated actions of the intracellular cytoskeleton – actin, microtubules (MTs) and substrate/focal adhesions (FAs). This coordination is altered in metastatic cancer cells resulting in deregulated and increased cellular motility. Microfabrication tools, including photolithography, micromolding, microcontact printing, wet stamping and microfluidic devices have emerged as a powerful set of experimental tools with which to probe and define the differences in cytoskeleton organization/dynamics and cell motility patterns in non-metastatic and metastatic cancer cells. In this review, we discuss four categories of microfabricated systems: (i) micropatterned substrates for studying of cell motility sub-processes (for example, MT targeting of FAs or cell polarization); (ii) systems for studying cell mechanical properties, (iii) systems for probing overall cell motility patterns within challenging geometric confines relevant to metastasis (for example, linear and ratchet geometries), and (iv) microfluidic devices that incorporate co-cultures of multiple cells types and chemical gradients to mimic in vivo intravasation/extravasation steps of metastasis. Together, these systems allow for creating controlled microenvironments that not only mimic complex soft tissues, but are also compatible with live cell high-resolution imaging and quantitative analysis of single cell behavior.

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Retracted: Guiding Cell Migration Using One‐Way Micropattern Arrays

Cited by 93 publications

References 57 publications

Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading

Kinetic behaviour of the cells touching substrate: the interfacial stiffness guides cell spreading

Intracellular forces during guided cell growth on micropatterns using FRET measurement

Microfabricated Systems and Assays for Studying the Cytoskeletal Organization, Micromechanics, and Motility Patterns of Cancerous Cells

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