Levetiracetam modulates hypoxia-induced inflammation and oxidative stress via inhibition of TRPV1 channel in the DBTRG glioblastoma cell line

Abstract

Levetiracetam modulates hypoxia-induced inflammation and oxidative stress via inhibition of TRPV1 channel in the DBTRG glioblastoma cell line Kemal Ertilav 895 IDH1 R132H gene mutation reduces cell proliferation and sensitizes recurrent Glioblastoma to hydrogen peroxide

Low molecular weight heparin treatment reduced apoptosis and oxidative cytotoxicity in the thrombocytes of patients with recurrent pregnancy loss and thrombophilia: Involvements of TRPM2 and TRPV1 channels

“…The inhibition of RPL‐induced increase of caspase‐3, and caspase ‐9 but the decrease of cell viability by the treatments of ACA and CPZ as well as the LMWH treatment suggested the important role of [Ca 2+ ] i , TRPM2, and TRPV1 in the induction of apoptosis. In addition to the stimulation of [Ca 2+ ] i increase dependent‐caspase‐3 and caspase‐9 pathways of apoptosis, [Ca 2+ ] i signal via the stimulation of TRPM2 and TRPV1 was found to alter the MiMP that might be critical for the induction of apoptosis 12,24–26,34,35 . Consistently, the results in the current study revealed that RPL increase MiMP levels, which was found to be attributable to the changes in the [Ca 2+ ] i concentration as evidenced by the preventive actions of ACA and CPZ, antagonists of TRPM2 and TRPV1.…”

“…The TRPM2 is activated by the PARP‐1 stimulation‐mediated ADP‐ribose generation 19,21,34 . Hence, we performed the PARP‐1 protein band levels in the thrombocytes of Ctr, patient, and treatment (LMWH) groups.…”

“…To determine active caspase activities, we used fluorogenic substrates of caspase‐3 (AC‐DEVD‐AMC) and caspase‐9 (AC‐LEHD‐AMC) 34,35 . Briefly, thrombocyte lysates were incubated with 2 mL substrate solution (as [mM]: 20 HEPES [20], EDTA [2], CHAPS [0.1%], dithiothreitol [5], and caspase substrates [8.25 μM]) for 1 h at 37°C.…”

“…The thrombocytes were incubated in the black plates with 2 μM DCFH‐DA at 37°C for 30 min. After washing the dyes, florescence intensity changes of the dye were analyzed in the plate reader by using the excitation (at 485 nm)/emission (at 535 nm) rate 34,35 . The data of DCF were presented as fold increase.…”

“…26 To determine active caspase activities, we used fluorogenic substrates of caspase-3 (AC-DEVD-AMC) and caspase-9 (AC-LEHD-AMC). 34,35 Briefly, thrombocyte lysates were incubated with 2 mL substrate solution (as [mM]: 20 HEPES [20], EDTA [2], CHAPS [0.1%], dithiothreitol [5], and caspase substrates [8.25 μM]) for 1 h at 37 C. The substrate cleavages of AC-DEVD-AMC and AC-LEHD-AMC were measured in the microplate reader (PRO200) by using the excitation (360 nm) and emission (460 nm) wavelengths.…”