Levels of Total Fucose and Total Protein in Sera of Blood Groups and RBCs of Control , Minor and Major Thalassemic Patients

Al-Hakeem, Tarik; Hasan, Hamid Gaffoori; Ali, Zeyan Abdullah

doi:10.5742/mejim.2013.62219

Cited by 1 publication

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“…L-fucose takes part in the fructose and mannose metabolism pathway (sugar metabolism) where it forms fuculose with the help of L-fucose isomerase. The level of L-fucose was increased in major thalassemia patients as compared to the minor thalassemia and control ones as already reported, especially in O-blood groups as compared to the A, B, and AB type blood groups which suggested severity and complexity in the growth of the disease [ 18 , 19 ]. Our results illustrated down-regulation of L-fucose, as shown in Figure 6 , in the β-YAC transgenic mice induced by TDF, though there was a slightly increased level in the samples induced with HU drug, suggesting TDF as a better HbF inducer for enhancing γ-globin gene activation.…”

Section: Discussionsupporting

confidence: 53%

Metabolomics Study of Serum Samples of β-YAC Transgenic Mice Treated with Tenofovir Disoproxil Fumarate

Kumari

Khan

Siddiqui

et al. 2022

IJMS

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β-thalassemia is one of the most common monogenic disorders and a life-threatening health issue in children. A cost-effective and safe therapeutic approach to treat this disease is to reactivate the γ-globin gene for fetal hemoglobin (HbF) production that has been silenced during infancy. Hydroxyurea (HU) is the only FDA approved HbF inducer. However, its cytotoxicity and inability to respond significantly in all patients pose a need for an HbF inducer with better efficacy. The study describes the serum metabolic alteration in β-YAC transgenic mice treated with Tenofovir disoproxil fumarate (TDF) (n = 5), a newly identified HbF inducer, and compared to the mice groups treated with HU (n = 5) and untreated control (n = 5) using gas chromatography-mass spectrometry. Various univariate and multivariate statistical analyses were performed to identify discriminant metabolites that altered the biological pathways encompassing galactose metabolism, lactose degradation, and inositol. Furthermore, the decreased concentrations of L-fucose and geraniol in TDF-treated mice help in recovering towards normal, decreasing oxidative stress even much better than the HU-treated mice. The proposed study suggested that TDF can reduce the deficiency of blood required for β-thalassemia and can be used for the preclinical study at phase I/II for fetal hemoglobin production.

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Section: Discussionsupporting

confidence: 53%