Levels of lipid peroxidation, nitric oxide, and antioxidant vitamins in plasma of patients with fibromyalgia

Akkuş, Selami; Nazıroğlu, Mustafa; Eriş, Sevilay; Yalman, Kadir; Yılmaz, Nigar; Yener, Mahmut

doi:10.1002/cbf.1548

“…Nonetheless, previous findings regarding NO levels in patients with FM have been inconsistent. Our results in the present study showed no significant differences in NO between either normal-weight or overweight women with FM and their age-and weightmatched controls, which is in accord with the results of a number of previous studies (Akkuş et al, 2009;Kim et al, 2010;Sendur, Turan, Tastaban, Yenisey, & Serter, 2009). However, we did note a pattern of increasing NO in normal-weight patients in comparison to the controls.…”

Section: Correlations Between Study Variables In Women Diagnosed With Fmsupporting

confidence: 96%

Nitric Oxide, Inflammation, Lipid Profile, and Cortisol in Normal- and Overweight Women With Fibromyalgia

Rus

¹

,

Molina

²

,

Gassó

³

et al. 2015

Biological Research For Nursing

View full text Add to dashboard Cite

show abstract

“…Akkus et al [3] showed a reduction of vitamin A in FMS patients as a result of its role on radical inhibition and hypothesized an imbalance between ROS production and the antioxidant defence system in patients with fibromyalgia syndrome. These data might be consistent with a previous study [9] that showed unexpected normal levels of uric acid (despite high levels of inosine, hypoxanthine, xanthine) in FMS patients possibly as a result of its role as a ROS scavenger.…”

Section: Fms (N = 16) Hc (N = 12)mentioning

confidence: 98%

“…Oxidative stress has been proposed as a relevant event in the pathogenesis of FMS with an increase of lipid peroxidation (LPO) [2] and a decrease in vitamin A and E concentrations [3].…”

mentioning

confidence: 99%

“…

Fibromyalgia syndrome (FMS) is a complex illness to diagnose and treat, which presents symptoms that may be part of, or overlap with other diseases or syndromes.The most widely used diagnostic criteria are those of the American College of Rheumatology [1]; no laboratory test has been validated for FMS diagnosis which remains primarily clinically based.Oxidative stress has been proposed as a relevant event in the pathogenesis of FMS with an increase of lipid peroxidation (LPO) [2] and a decrease in vitamin A and E concentrations [3].Although the etiology of FMS remains unclear, genetic predisposition is likely to be an important factor and transmission is thought to be polygenic [4,5]. If FMS is a multi-genetic disease then it could be hypothesized that differences exist in the type of proteins or protein expression levels in sera of FMS patients compared with healthy controls.

Proteomic analysis is a powerful tool for the global evaluation of protein expression and plays a central role in clinical diagnosis and monitoring.

…”

mentioning

confidence: 99%

See 1 more Smart Citation

A preliminary study on serum proteomics in fibromyalgia syndrome

Ruggiero¹,

Era²,

Cacace³

et al. 2014

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Fibromyalgia syndrome (FMS) is a complex illness to diagnose and treat, which presents symptoms that may be part of, or overlap with other diseases or syndromes.The most widely used diagnostic criteria are those of the American College of Rheumatology [1]; no laboratory test has been validated for FMS diagnosis which remains primarily clinically based.Oxidative stress has been proposed as a relevant event in the pathogenesis of FMS with an increase of lipid peroxidation (LPO) [2] and a decrease in vitamin A and E concentrations [3].Although the etiology of FMS remains unclear, genetic predisposition is likely to be an important factor and transmission is thought to be polygenic [4,5]. If FMS is a multi-genetic disease then it could be hypothesized that differences exist in the type of proteins or protein expression levels in sera of FMS patients compared with healthy controls.Proteomic analysis is a powerful tool for the global evaluation of protein expression and plays a central role in clinical diagnosis and monitoring. Some studies [6] reported interesting proteomic analysis data with an overexpression of transaldolase and phosphoglicerate mutase I in salivary fluid of FMS patients and focused on the role of oxidative stress in the pathogenesis of the disease.The aim of this preliminary study was to evaluate, using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS), changes in protein profiles of FMS patients with respect to control subjects to identify potential serum biomarkers useful for disease diagnosis and management.Sixteen females (52 ± 12 years) affected by FMS and 12 healthy females (48 ± 13 years) were enrolled; all patients fulfilled new ACR diagnostic criteria [1] ( Table 1). The presence of a major clinical condition other than fibromyalgia was excluded by physical examination and routine blood and urine screening. No patients suffered of thyroid dysfunction. Psychiatric examination data of patients were reported in a previous study [7].Study was approved from our Local Ethical Committee and informed consent was obtained from all subjects.Serum protein concentrations were quantified using the PlusOne 2D Quant Kit (GE Healthcare); first dimension isoelectric focusing (IEF) was carried out with 18 cm immobilized pH gradient strips of pH 3-10. The IPG strips were applied onto 10% acrylamide sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) slab gels (25.5 cm × 20.5 cm × 1.0 mm) and overlaid with a solution of 0.5% agarose with a trace of bromophenol blue. Gels were fixed, stained with Coomassie Brilliant Blue G-250 colloidal for 24 h and stored at room temperature. At least three sample replicates were performed.2D data were processed with Progenesis SameSpots software, which computed multiplication fold, false discovery rate (FDR) q-value, p-values of all spots using one-way ANOVA analysis. A p-value < 0.05 was considered statistically significant; no difference has been found in protein expression according to patient therapy.After normalization, volume calcul...

show abstract

“…Many studies investigated the role of NO in the etiology of FMS [42,43], where FMS has been described as a disease characterized by abnormal sensitization of the spinal cord and CNS, this sensitization may occur because of inflammatory cytokines that trigger inducible nitric oxide synthase (iNOS) in muscle tissues; this isoform causes inappropriate stimulation of pain receptors and an increase in oxidants such as the peroxynitrite radical and other ROS [44]. Previous studies have reported such oxidative damage in FMS, which are consistent with the proposed idea [45,46].…”

Section: Discussionmentioning

confidence: 99%