Levels of IgM antibodies against dengue virus in Rio de Janeiro, Brazil

Diagnosis of Dengue by Using Reverse Transcriptase-Polymerase Chain Reaction

“…Different studies have shown a range from 12% to 100% of virus detection in serologically confirmed cases (Chungue et al 1993, Chan et al 1994). The inverse correlation between virus isolation and anti-dengue antibodies has been well documented (Nogueira et al 1992). In agreement with Chan et al (1994), our results also showed that RT-PCR was most useful when anti-dengue antibodies were undetectable.…”

“…Enzyme-linked immunosorbent assay (Elisa) has proven to be a sensitive method for detecting anti-dengue IgM (Nogueira et al 1992), however a monotypic response is rarely observed because of the extensive cross-reactivity among dengue viruses. In our study, RT-PCR identified dengue virus RNA in 32.4% and 59.4% of cases respectively in relation to the presence or absence of antidengue IgM antibodies.…”

“…IgM capture enzyme-linked immunosorbent assay (MAC-ELISA) was performed using a mixture of DEN-1 and DEN-2 antigens according to Nogueira et al (1992).…”

Clinical and epidemiological aspects of human parvovirus B19 infection in an urban area in Brazil (Niterói city area, State of Rio de Janeiro, Brazil)

“…The samples were centrifuged and serum was separated and frozen at -20 o C until the serological analysis was performed. All serum samples were tested for the presence of anti-rubella IgM virus antibodies by using a commercial enzyme immunoassay (EIA) (Rubenostika IgM, Organon), for anti-measles virus IgM by using an antibody capture EIA developed at the Centers for Disease Control and Prevention (Atlanta, USA) (Hummel et al 1992), and for anti-dengue virus IgM by using an in-house EIA (Kuno et al 1987, Nogueira et al 1992. Those specimens, negative for rubella, measles and dengue virus antibodies, were also tested for anti-human parvovirus B19 IgM using an antibody capture EIA (MACEIA) (Cubel et al 1994, Nascimento et al 1998.…”

The impact of dengue on liver function as evaluated by aminotransferase levels

“…Immunoenzymatic tests (ELISA) are an alternative for confirmation of the diagnosis and are available in the laboratories of the public healthcare network. These tests permit confirmation in samples taken from the 7 th day of the disease onwards in around 80% of cases, achieving as high as 100% of cases after two weeks [6].…”