Levels of Gingival Crevicular Metalloproteinases-8 and -9 in Periodontitis

Rai, Balwant

doi:10.4034/1519.0501.2008.0083.0014

Cited by 7 publications

(9 citation statements)

References 19 publications

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“…Specifically, MMP-2 is a gelatinase targeting collagen type IV, which is distributed in the base membranes of gingival connective tissues [19]. In addition, MMP-8 is a collagenase degrading collagen type I and III, which are the principal organic constituents found in the periodontal tissues [20]. Therefore, MMP-2 and MMP-8 might be responsible for the destruction of the free gingival tissues as well as the loss of periodontal ligaments, resulting in the deepening of the gingival sulcus.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effects of Panduratin A on Periodontitis-Induced Inflammation and Osteoclastogenesis through Inhibition of MAPK Pathways In Vitro

Kim¹,

Kim²,

Kim³

et al. 2018

Journal of Microbiology and Biotechnology

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Periodontitis is an inflammatory disease caused by microbial lipopolysaccharide (LPS), destroying gingival tissues and alveolar bone in the periodontium. In the present study, we evaluated the anti-inflammatory and anti-osteoclastic effects of panduratin A, a chalcone compound isolated from , in human gingival fibroblast-1 (HGF-1) and RAW 264.7 cells. Treatment of panduratin A to LPS-stimulated HGF-1 significantly reduced the expression of interleukin-1β and nuclear factor-kappa B (NF-κB), subsequently leading to the inhibition of matrix metalloproteinase-2 (MMP-2) and MMP-8 compared with that in the LPS control (** < 0.01). These anti-inflammatory responses were mediated by suppressing the mitogen-activated protein kinase (MAPK) signaling and activator protein-1 complex formation pathways. Moreover, receptor activator of NF-κB ligand (RANKL)-stimulated RAW 264.7 cells treated with panduratin A showed significant inhibition of osteoclastic transcription factors such as nuclear factor of activated T-cells c1 and c-Fos as well as osteoclastic enzymes such as tartrate-resistant acid phosphatase and cathepsin K compared with those in the RANKL control (** < 0.01). Similar to HGF-1, panduratin A suppressed osteoclastogenesis by controlling MAPK signaling pathways. Taken together, these results suggest that panduratin A could be a potential candidate for development as a natural anti-periodontitis agent.

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Section: Discussionmentioning

confidence: 99%

Inhibitory Effects of Panduratin A on Periodontitis-Induced Inflammation and Osteoclastogenesis through Inhibition of MAPK Pathways In Vitro

Kim¹,

Kim²,

Kim³

et al. 2018

Journal of Microbiology and Biotechnology

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“…53,54 MMP-9 is an important indicator of the severity and progression of periodontitis. [55][56][57][58] IL-1β upregulates MMP-9 expression in various cell types involved in periodontal inflammation, including osteoblasts, osteoclasts, neutrophils, and cementoblasts. 59,60 In addition, IL-1β stimulated the production of other MMPs, e.g., MMP-1 and/or MMP-3, in human periodontal ligament cells and gingival fibroblast cells.…”

Section: The Role Of Il-1β In Periodontitismentioning

confidence: 99%

Interleukin-1β is a potential therapeutic target for periodontitis: a narrative review

et al. 2020

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Interleukin(IL)-1β, a pro-inflammatory cytokine, was elevated and participates in periodontitis. Not only the link between IL-1β and periodontitis was proved by clinical evidence, but also the increased IL-1β triggers a series of inflammatory reactions and promotes bone resorption. Currently, IL-1β blockage has been therapeutic strategies for autoimmune and autoinflammatory diseases such as rheumatoid arthritis, cryopyrin-associated periodic syndromes, gout and type II diabetes mellitus. It is speculated that IL-1β be a potential therapeutic target for periodontitis. The review focuses on the production, mechanism, present treatments and future potential strategies for IL-1β in periodontitis.

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“…The role of fibroblasts is not only to secrete the components of the extracellular matrix (ECM) such as collagen, fibronectin and proteoglycan (Graham et al 2008;Spinale 2007), but also to secrete ECM-degrading enzymes called matrix metalloproteases (MMPs) (Kukacka et al 2005;Lindner et al 2012). In the case of periodontal fibroblasts, it is known that they release MMP-2, 8 and 9 (Kim et al 2012;Lisboa et al 2009;Rai et al 2010). The MMPs may degrade the collagen of the membrane, resulting in a lack of attachment cues for the cells, thereby promoting cellular detachment and eventually, cellular death.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Human Amniotic Membrane as a Scaffold for Periodontal Ligament Fibroblast Attachment and Proliferation

Elahi¹,

Taib²,

Berahim³

et al. 2019

JSM

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This study was aimed at evaluating the ability of the human amniotic membrane (HAM) to act as a scaffold for the growth of the main cells in periodontal regeneration, human periodontal ligament fibroblasts (HPDLFs). The HAM has many biological properties that are suitable for periodontal tissue regeneration such as low immunogenicity, anti-fibrosis, anti-inflammation, and a rich extracellular matrix component. Commercially available HPDLFs were seeded onto the HAM, and the attachment and proliferation of the cells were observed through scanning electron microscopy (SEM) and histological analysis. Cell viability was assessed using the alamarBlue® proliferation assay at days 1, 3, 7, 14 and 21. Histologically, the HPDLFs showed a monolayer to multilayer attachment onto the HAM from day 1 to day 7. The SEM analysis demonstrated that the HPDLFs had attached appropriately onto the HAM surface at day 1 to day 3, and began overlapping at day 7, while maintaining their flat shape. However, by days 14 and 21, there was an alteration in the morphology of the cells, where they later became rounded. The proliferation assay showed that the viability of the HPDLFs on the HAM had increased significantly from day 1 to day 7 (p=0.012), but later showed significant reduction at day 14 (p=0.002) and day 21 (p=0.005). In conclusion, this study showed that the HAM was able to function well as a scaffold for HPDLFs within 7 days, and thus, it can be a promising scaffold for periodontal regeneration. However, the behaviour of the cells in relation to the membrane over longer culture duration warrants further investigation. ABSTRAK Kajian ini bertujuan untuk menilai keupayaan membran amniotik manusia (HAM) untuk bertindak sebagai perancah bagi pertumbuhan sel-sel utama dalam regenerasi periodontal iaitu sel fibroblas ligamen periodontal manusia (HPDLFs). HAM mempunyai banyak ciri biologi yang sesuai untuk pertumbuhan semula tisu periodontal seperti keimunogenan yang rendah, anti-fibrosis, anti-keradangan dan kaya dengan komponen matriks luar sel. HPDLFs yang tersedia secara komersial telah dibiakkan ke atas HAM, dan pelekatan serta pengembangbiakan HPDLFs diperhatikan melalui pemeriksaan mikroskop imbasan elektron (SEM) dan analisis histologi. Daya tahan sel telah dinilai menggunakan ujian percambahan alamarBlue® pada hari 1, 3, 7, 14 dan 21. Secara histologi, HPDLFs menunjukkan pelekatan ekalapisan kepada beberapa lapisan ke atas HAM daripada hari pertama hingga ke-7. Analisis SEM menunjukkan bahawa HPDLFs telah melekat dengan baik pada permukaan HAM pada hari pertama hingga hari ke-3 dan mula bertindih pada hari ke-7, sambil mengekalkan bentuknya yang rata. Bagaimanapun, pada hari ke-14 dan ke-21, terdapat perubahan pada morfologi sel, dengan mereka kemudiannya menjadi bulat. Ujian percambahan menunjukkan bahawa daya tahan sel HPDLFs pada HAM meningkat dengan ketara daripada hari pertama hingga hari ke-7 (p=0.012) namun kemudiannya menunjukkan penurunan yang ketara pada hari ke-14 (p=0.002) dan hari ke-21 (p=0.005). Kesimpulannya, kaj...

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Levels of Gingival Crevicular Metalloproteinases-8 and -9 in Periodontitis

Cited by 7 publications

References 19 publications

Inhibitory Effects of Panduratin A on Periodontitis-Induced Inflammation and Osteoclastogenesis through Inhibition of MAPK Pathways In Vitro

Inhibitory Effects of Panduratin A on Periodontitis-Induced Inflammation and Osteoclastogenesis through Inhibition of MAPK Pathways In Vitro

Interleukin-1β is a potential therapeutic target for periodontitis: a narrative review

Evaluation of the Human Amniotic Membrane as a Scaffold for Periodontal Ligament Fibroblast Attachment and Proliferation

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