Levels of bPSPB throughout single and twin pregnancies after AI or transfer of IVM/IVF cattle embryos

Vasques, M.I.; Horta, A.E.M.; Marques, C.C.; Sasser, R.G.; Humblot, P.

doi:10.1016/0378-4320(94)01373-t

Cited by 42 publications

(34 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Placental BC products, such as bPL, bPSPB and bPAG-1, have been suggested as useful markers for embryonic or placental health, pregnancy normalcy and embryonic mortality, and for the prediction of abnormal conceptus development and postpartum pathological events (Patel et al 1997, Szenci et al 1998, Tefera et al 2001. Such BC products or the relative abundances of their placental transcripts have already been used in an attempt to detect or predict conceptus abnormalities after in vitro embryo manipulations (Vasquez et al 1995, Schmidt et al 1996, Hill et al 2000, Hashizume et al 2002, Heyman et al 2002, Ishiwata et al 2003, Patel et al 2004a, Ravelich et al 2004, and results from these studies have increasingly contributed to the understanding of those abnormalities.…”

Section: Discussionmentioning

confidence: 99%

“…The amounts of placental products in maternal circulation appear to be associated with placental and foetal masses, including foetal number and neonatal birth weight, and with pregnancy normalcy (Byatt et al 1992a, Patel et al 1995, Vasquez et al 1995 2001, Gootwine 2004). In this study, concentrations of maternal and foetal plasma bPL followed similar trends reported by Hossner et al (1997), Holland et al (1997) and Patel et al (1996), but concentrations of bPL and bPSPB in foetal plasma were correlated with conceptus physical traits and with one another in plasma and fluids.…”

Section: Discussionmentioning

confidence: 99%

“…The amounts of BC products in maternal circulation appear to be associated with placental mass, foetal number and neonatal birth weight, and have been postulated to be an index for conceptus viability and pregnancy normalcy in cattle (Byatt et al 1992a, Patel et al 1995, Vasquez et al 1995, Szenci et al 1998, Tefera et al 2001, Ravelich et al 2004. Associations between abnormal placental and foetal development after in vitro embryo manipulations have been suggested to play a key role in the occurrence of high birth weights.…”

Section: Introductionmentioning

confidence: 99%

“…Despite the unknown function and control of PSPB secretion, many potential biological functions have been suggested based on in vivo and in vitro effects, including prostaglandin release by the endometrium, implantation, immunotolerance of the conceptus as a tissue alograft and uterine remodelling after parturition, among others (Del Vecchio et al 1990, Kiracofe et al 1993, Austin et al 1999, Tefera et al 2001. Both PAG-1 and PSPB diagnostic assays have been developed for early plasma diagnosis of pregnancy in cattle and other ruminants (Sasser et al 1986, Zoli et al 1991, Green et al 2000.The amounts of BC products in maternal circulation appear to be associated with placental mass, foetal number and neonatal birth weight, and have been postulated to be an index for conceptus viability and pregnancy normalcy in cattle (Byatt et al 1992a, Patel et al 1995, Vasquez et al 1995, Szenci et al 1998, Tefera et al 2001, Ravelich et al 2004. Associations between abnormal placental and foetal development after in vitro embryo manipulations have been suggested to play a key role in the occurrence of high birth weights.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Expression profile and protein levels of placental products as indirect measures of placental function in in vitro-derived bovine pregnancies

Bertolini¹,

Wallace²,

Anderson³

2006

Reproduction

View full text Add to dashboard Cite

Bovine conceptus development and its association with placental proteins present in maternal, foetal and neonatal plasma and foetal (amniotic and allantoic) fluids were investigated in in vivo-and in vitro-produced (IVP) concepti and newborn calves. Females were superovulated to obtain control embryos, whereas IVP embryos were derived from established in vitro procedures. Pregnant animals were slaughtered on days 90 or 180 of gestation or allowed to develop to term for the assessment of physical traits. Foetal, maternal and neonatal blood and foetal fluids were collected for the determination of bovine placental lactogen (bPL) and bovine pregnancy-specific protein B (bPSPB) concentrations. Placental transcripts for bPL and bPSPB, determined by quantitative RT-PCR, were elevated in IVP placentomes. No major physical differences were observed between groups on day 90, but concentrations of bPL and bPSPB were higher in foetal plasma and allantoic fluid of IVP concepti in day 180 pregnancies, which were correlated with larger uterine and conceptus traits. Maternal concentrations of bPL in IVP pregnancies were lower than controls during the last 8 weeks of gestation, to become similar as parturition approached. Newborn IVP calves and foetal membranes were larger and displayed higher concentrations of plasma bPL than controls (10 and 60 min after birth). Our results indicated that differential patterns of secretion of bPL and bPSPB into the maternal and foetal systems occurred at distinct stages of gestation, and these were associated with altered conceptus development after in vitro embryo manipulations, indirectly demonstrating deviations in placental function in IVP pregnancies. Reproduction (2006) 131 163-173 IntroductionWith the onset of the trophoblast apposition to the endometrium during the process of bovine placenta formation and development, large granulated cells, the trophoblast binucleate cells (BCs), migrate from the trophectoderm to fuse with uterine epithelial cells (Wooding 1992), forming short-lived trinucleate cells. This phenomenon occurs throughout pregnancy in cattle, but the number and period of existence of trinucleate cells decline towards the end of gestation (Wooding 1992, Green et al. 2000. Binucleate cells comprise about 20% of the trophoblast cells and are responsible for hormones and bioactive products important for the regulation of foetal growth and development (Anthony et al. 1995, Green et al. 2000, Schlafer et al. 2000. The contents of the membranebounded secretory granules of BCs are released by exocytosis towards the maternal capillary bed, reaching the maternal blood system (Wooding 1992). Many proteins are produced by the BCs, including placental lactogen (PL) and a range of glycoproteins (Wooding 1992), such as pregnancy-specific protein B (PSPB) (Butler et al. 1982).PL lactogen is a member of the growth hormone (GH)/prolactin (PRL) gene family synthesized and stored in secretory granules of the trophoblast BCs (Byatt et al. 1992a, Wooding 1992, Gootwine 2004. Although ...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Expression profile and protein levels of placental products as indirect measures of placental function in in vitro-derived bovine pregnancies

Bertolini¹,

Wallace²,

Anderson³

2006

Reproduction

View full text Add to dashboard Cite

show abstract

“…Bovine PSPB [21] and bPAG1 [20] are inactive aspartic acid proteinases, and are identical in genetic nucleotide sequence [22][23][24]. The isolated preparations of bPSPB and bPAG1 may differ in carbohydrate and sialic acid content, which may explain the minor differences in profile and disappearance from maternal circulation after calving or EM [15, 25,26].…”

Section: Conceptus Proteins (Pspb Pag)mentioning

confidence: 99%

Recent Possibilities for the Diagnosis and Pharmacological Control of Pregnancy Loss in Dairy Cow

Szenci¹

2015

JLS

View full text Add to dashboard Cite

Abstract:One of the most recent techniques for the diagnosis of EP (early pregnancy) in cattle on the farm is B-mode ultrasonography. Under field conditions, acceptable results may be achieved with ultrasonography from days 25 to 30 post-AI. The reliability of the test greatly depends on the frequency of the transducer used, the skill of the examiner, the criterion used for a positive PD (pregnancy diagnosis) and the position of the uterus in the pelvic inlet. Pregnancy protein assays (PAG, PSPB) may provide an alternative method to ultrasonography for determining EP or LEM/EFM (late embryonic/early foetal mortality) in the cow. Although early pregnancy factor is the earliest specific indicator for fertilization, its detection is entirely dependend on the use of the RIT (rosette inhibition test), therefore its use in the field needs further inventions. Preventive pharmaceutical treatments with hCG (human chorionic gonadotropin), GnRH (gonadotropin realising hormone), PRID (progesterone-releasing intravaginal device) or CIDR (controlled internal drug release) inserts at different time periods at or post-AI may have some benefit in decreasing PL (pregnancy loss), however further examinations are warranted to determine how and when these treatments may influence PL in the field.

show abstract

Effect of ambient lead on progesterone and pregnancy-associated glycoprotein 1 and their relationship with abortion in Zaraibi goats: a field study

Mosaad,

El-Nakhla,

Abd El-Rasoul

et al. 2024

Trop Anim Health Prod

View full text Add to dashboard Cite

This study aimed to investigate the impact of ambient lead (Pb) exposure on progesterone (P4) and pregnancy-associated glycoprotein 1 (PAG1) and their relationship with abortion in Egyptian Zaraibi goats (C. hircus). To achieve this, 40 female goats (does) were mated with highly fertile male goats, resulting in a total of 28 pregnant goats. Eight of them aborted, and each of the 12 pregnant goats gave birth to one kid, whereas the remaining eight gave birth to twins. The levels of PAG1, P4, and Pb in serum were estimated by enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), and inductively coupled plasma mass spectrometry (ICP-MS) respectively. Statistically, the repeated measure two-way ANOVA, regression analysis, correlation coefficient, and receiver operating characteristic (ROC) curves were applied. The current data demonstrated that the levels of blood Pb in aborted goats were significantly higher than those in non-aborted goats at the early, mid, and late gestations, and this was followed by significant decreases in serum PAG1 and P4. Furthermore, there were substantial inverse associations between blood Pb concentration and levels of PAG1 and P4, with markedly negative correlation coefficients of − 0.88 and − 0.77, respectively, in aborted goats. The threshold level of Pb required to cause abortion was ≥ 32.08 μg/dl, but for PAG1 and P4 were respectively ≤ 0.95 ng/ml and ≤ 0.48 ng/ml. Additionally, threshold levels of ≥ 12.34 ng/ml and ≥ 31.52 ng/ml for P4 and PAG1, respectively, were needed to deliver twins. In conclusion, pollution-induced increases in Pb bioavailability resulted in dramatic decreases in P4 and PAG1 levels, leading to abortions. PAG1 and P4 levels are also key factors in determining whether Zaraibi goats will give birth to twins.

show abstract

Levels of bPSPB throughout single and twin pregnancies after AI or transfer of IVM/IVF cattle embryos

Cited by 42 publications

References 24 publications

Expression profile and protein levels of placental products as indirect measures of placental function in in vitro-derived bovine pregnancies

Expression profile and protein levels of placental products as indirect measures of placental function in in vitro-derived bovine pregnancies

Recent Possibilities for the Diagnosis and Pharmacological Control of Pregnancy Loss in Dairy Cow

Effect of ambient lead on progesterone and pregnancy-associated glycoprotein 1 and their relationship with abortion in Zaraibi goats: a field study

Contact Info

Product

Resources

About