Level of N6-Methyladenosine in Peripheral Blood RNA: A Novel Predictive Biomarker for Gastric Cancer

Ge, Lichen; Zhang, Nan; Chen, Zhuojia; Song, Jiuzhou; Wu, Yinuo; Li, Zhuoling; Chen, Feng; Wu, Jia; Li, Dandan; Li, Jiexin; Wang, Cheng; Wang, Hongsheng; Wang, Junjun

doi:10.1093/clinchem/hvz004

Cited by 57 publications

(55 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a kind of chemical modifications in RNA, the alterations of their regulations may also predict the prognosis or be used for diagnosis of GBM. Actually, m 6 A in in peripheral blood RNA has shown to be a predictive biomarker for gastric cancer [165]. Methylation levels in miRNAs are also potential diagnostic biomarkers for early-stage gastrointestinal cancers [166].…”

Section: Potential Diagnostic Implications Of Rna Modifications In Gbmmentioning

confidence: 99%

The Emerging Roles of RNA Modifications in Glioblastoma

Dong

Cui

2020

Cancers

View full text Add to dashboard Cite

Glioblastoma (GBM) is a grade IV glioma that is the most malignant brain tumor type. Currently, there are no effective and sufficient therapeutic strategies for its treatment because its pathological mechanism is not fully characterized. With the fast development of the Next Generation Sequencing (NGS) technology, more than 170 kinds of covalent ribonucleic acid (RNA) modifications are found to be extensively present in almost all living organisms and all kinds of RNAs, including ribosomal RNAs (rRNAs), transfer RNAs (tRNAs) and messenger RNAs (mRNAs). RNA modifications are also emerging as important modulators in the regulation of biological processes and pathological progression, and study of the epi-transcriptome has been a new area for researchers to explore their connections with the initiation and progression of cancers. Recently, RNA modifications, especially m6A, and their RNA-modifying proteins (RMPs) such as methyltransferase like 3 (METTL3) and α-ketoglutarate-dependent dioxygenase alkB homolog 5 (ALKBH5), have also emerged as important epigenetic mechanisms for the aggressiveness and malignancy of GBM, especially the pluripotency of glioma stem-like cells (GSCs). Although the current study is just the tip of an iceberg, these new evidences will provide new insights for possible GBM treatments. In this review, we summarize the recent studies about RNA modifications, such as N6-methyladenosine (m6A), N6,2′O-dimethyladenosine (m6Am), 5-methylcytosine (m5C), N1-methyladenosine (m1A), inosine (I) and pseudouridine (ψ) as well as the corresponding RMPs including the writers, erasers and readers that participate in the tumorigenesis and development of GBM, so as to provide some clues for GBM treatment.

show abstract

Section: Potential Diagnostic Implications Of Rna Modifications In Gbmmentioning

confidence: 99%

The Emerging Roles of RNA Modifications in Glioblastoma

Dong

Cui

2020

Cancers

View full text Add to dashboard Cite

show abstract

“…For instance, a vitro experiment proved that m 6 A suppression promoted GC cell proliferation and invasiveness through activating Wnt and PI3K-Akt signaling, while m 6 A elevation reversed these phenotypical and molecular changes [ 8 ]. Another research claimed that the level of m 6 A in peripheral blood RNA was a promising noninvasive diagnostic biomarker for GC patients [ 5 ]. Therefore, the exploration for m 6 A patterns would deepen our insights into RNA posttranscriptional regulatory network participating in the complex biological processes implicated in cancer.…”

Section: Discussionmentioning

confidence: 99%

“…The exploration on methylation patterns in tissue and cells could not only reveal the specific distribution of m 6 A modification in numerous transcripts, but also uncover the differences in m 6 A status under physiological and pathophysiological conditions [ 4 ]. Therefore, in-depth knowledge of m 6 A methylome profile has great benefit for elucidating the development of various human diseases especially malignancy [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, the regulatory roles of m 6 A methylation in gastric cancer (GC) has been paid increasing attention, which is the fifth most common cancer worldwide and the third leading cause among cancer-related deaths [ 7 ]. The m 6 A modification might be closely associated with GC genesis and progression [ 5 , 8 ]. Previously, several studies have investigated some m 6 A or expression patterns of m 6 A-related genes in GC.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Joint analysis of lncRNA m6A methylome and lncRNA/mRNA expression profiles in gastric cancer

Sun

et al. 2020

Cancer Cell Int

View full text Add to dashboard Cite

Background N6-methyladenosine (m6A) modification might be closely associated with the genesis and development of gastric cancer (GC). Currently, the evidence established by high-throughput assay for GC-related m6A patterns based on long non-coding RNAs (lncRNAs) remains limited. Here, a joint analysis of lncRNA m6A methylome and lncRNA/mRNA expression profiles in GC was performed to explore the regulatory roles of m6A modification in lncRNAs. Methods Three subjects with primary GC were enrolled in our study and paired sample was randomly selected from GC tissue and adjacent normal tissue for each case. Methylated RNA Immunoprecipitation NextGeneration Sequencing (MeRIP-Seq) and Microarray Gene Expression Profiling was subsequently performed. Then co-expression analysis and gene enrichment analysis were successively conducted. Results After data analysis, we identified 191 differentially m6A-methylated lncRNAs, 240 differentially expressed lncRNAs and 229 differentially expressed mRNAs in GC. Furthermore, four differentially m6A-methylated and expressed lncRNAs (dme-lncRNAs) were discovered including RASAL2-AS1, LINC00910, SNHG7 and LINC01105. Their potential target genes were explored by co-expression analysis. And gene enrichment analysis suggested that they might influence the cellular processes and biological behaviors involved in mitosis and cell cycle. The potential impacts of these targets on GC cells were further validated by CCLE database and literature review. Conclusions Four novel dme-lncRNAs were identified in GC, which might exert regulatory roles on GC cell proliferation. The present study would provide clues for the lncRNA m6A methylation-based research on GC epigenetic etiology and pathogenesis.

show abstract

“…As an important chemical modification to RNA, regular changes in m6A can also predict the prognosis of patients with GBM or be used for the diagnosis of GBM. Related studies have shown that m6A in RNA from peripheral blood is a biomarker of gastric cancer [114]. The methylation level of miRNAs is also a potential diagnostic biomarker for early gastrointestinal cancer [115].…”

Section: Clinical Significance Of M6a Modification In Gliomas M6a Modmentioning

confidence: 99%

m6A modification in RNA: biogenesis, functions and roles in gliomas

Zhang

Geng

et al. 2020

J Exp Clin Cancer Res

109

View full text Add to dashboard Cite

The chemical modification of RNA is a newly discovered epigenetic regulation mechanism in cells and plays a crucial role in a variety of biological processes. N6-methyladenine (m6A) mRNA modification is the most abundant form of posttranscriptional RNA modification in eukaryotes. Through the development of m6A RNA sequencing, the relevant molecular mechanism of m6A modification has gradually been revealed. It has been found that the effect of m6A modification on RNA metabolism involves processing, nuclear export, translation and even decay. As the most common malignant tumour of the central nervous system, gliomas (especially glioblastoma) have a very poor prognosis, and treatment efficacy is not ideal even with the application of high-intensity treatment measures of surgery combined with chemoradiotherapy. Exploring the origin and development mechanisms of tumour cells from the perspective of tumour biogenesis has always been a hotspot in the field of glioma research. Emerging evidence suggests that m6A modification can play a key role in gliomas through a variety of mechanisms, providing more possibilities for early diagnosis and targeted therapy of gliomas. The aim of the present review is to focus on the research progress regarding the association between m6A modification and gliomas. And to provide a theoretical basis according to the currently available literature for further exploring this association. This review may provide new insights for the molecular mechanism, early diagnosis, histologic grading, targeted therapy and prognostic evaluation of gliomas.

show abstract

Level of N6-Methyladenosine in Peripheral Blood RNA: A Novel Predictive Biomarker for Gastric Cancer

Cited by 57 publications

References 28 publications

The Emerging Roles of RNA Modifications in Glioblastoma

The Emerging Roles of RNA Modifications in Glioblastoma

Joint analysis of lncRNA m6A methylome and lncRNA/mRNA expression profiles in gastric cancer

m6A modification in RNA: biogenesis, functions and roles in gliomas

Contact Info

Product

Resources

About