Objective: This study was planned to determine the level of knowledge about breast cancer (BC) and attitudes and practices towards BC in female healthcare providers. Thereby, it was aimed to identify the level of awareness, which is intended to be improved in the community, among female healthcare providers; the topics of failure; attitudes and practices towards screening programs; and factors involved in these areas. Method: The study sample consisted of 266 female healthcare providers. In all participants, a 29-item questionnaire was completed by the face-to-face interview method. The questionnaire included items about sociodemographic characteristics, and level of knowledge, attitudes and practices in BC. Each correct answer was rated as one point while no score was assigned to incorrect answers which were excluded. Data were analyzed by SPSS version 22.0 (SPSS Inc., Chicago, IL, USA). Results: The mean age was 30.29 ± 0.55 years. Of subjects, 47.7% (n=127) were physicians, 48.1% (n=128) were nurses while 4.2% (n=11) were other healthcare providers. Significant differences were found in the level of knowledge according to marital status, educational level and age. Of the factors affecting participation in screening programs, marital status, educational level, age, and duration of a professional career. It was found that the level of knowledge regarding BC was high while the participation rate for screening programs was low among subjects.Conclusion: It was found that knowledge level was high while the participation rate for screening programs were rather low among female healthcare providers. This shows that there should be studies in order to increase the participation of female healthcare providers in screening programs.