Levan Production Potentials From Different Hypersaline Environments in Turkey

Abstract

12 halophilic strains from different hypersaline environments such as solar salterns in Tuzlagözü (Sivas), Fadlum (Sivas), Kemah (Erzincan), a hypersaline spring water in Pülümür (Tunceli) and a saline lake in Delice (Kırıkkale) belonging to Turkey, were investigated in terms of levan production. After incubation and ethyl alcohol treatment, dialysis process was operated for partial purification. Levan amounts in our samples after hydrolysis were calculated based on the amount of sugar obtained by acid hydrolysis of standard levan. Sugar amount of samples were determined using by high performance liquid chromatography system (HPLC). 1H-Nuclear Magnetic Resonance (1H-NMR) spectra of the levan sample and standard were recorded. The results obtained by HPLC analysis showed that Chromohalobacter canadensis strain 85B had highest production potential as 234.67 mg levan/g biomass. The chemical shifts of 1H-NMR spectrum of the extracted levan also showed high similarity to those of pure levan isolated from Erwinia herbicola. Furthermore, Marinobacter sp. 163Y strain also is also capable with regard to levan production. In this study, this strain could yield 230.80 mg/g levan and this potential was first reported in the literature.

Biosynthesis of Levan by Halomonas elongata 153B: Optimization for Enhanced Production and Potential Biological Activities for Pharmaceutical Field

“…The synthesis of levan by a halophilic bacterium, Halomonas elongata 153B, isolated from a hypersaline environment, was examined in this work. In the study conducted by our group, the abilities of 12 halophilic strains to produce levan were screened and it was shown that the H. elongata 153B used in our study produced the high-yield levan [34].…”

Levan-based bioactive hydrogels containing herbal liposome in treating acute wounds

“…Especially through halophilic bacteria, the biopolymer can be produced with high efficiency in non-sterile conditions, and thus production costs can be reduced. 19,20 There has been an increasing trend toward herbal wound care products that include therapeutics such as extracts from medicinal plants, flavonoids, phenolic compounds and terpenoids in order to regenerate cells, ensure tissue integrity in a short time and reduce infections in the wound area. [21][22][23][24] These components are preferred over synthetic drug agents due to their easy availability from nature, low cost, limited side effects, high bioavailability, and efficacy in treatment.…”