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Boron is a vital micronutrient for the growth of plants and different organisms. However, boron may be toxic to living cells at high concentrations. Nevertheless, microorganisms can be grown in harsh conditions such as environments with higher boron concentrations. It is also known that boron-tolerant microorganisms have significant biotechnological potentials. In this study, it is aimed to isolate microorganism from a boron containing place in Kırka, Eskişehir using culturedependent methods. After extracting genomic DNAs, Amplified Ribosomal DNA Restriction Analysis (ARDRA) profiles were constructed with HaeIII, MspI, HinfI-HpaII and AluI-CfoI. Sequence analyses were performed by chain-terminating method. Consensus sequences were completed and similarities were determined by RDP Release 11. According to ARDRA, 10 profiles and five different species such as Pseudomonas sp., Enterecoccus sp., Acinetobacter sp., Bacillus sp. and Halomonas sp. were identified.

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Since it can be a source of new microorganisms with biological potential, it is important to identify the microorganisms found in environments with high boron content in terms of ecological and biotechnological application potentials. In this context, deposit waters in environments where boron mining activities perform are important habitats for boronophilic/boronotolerant microorganisms.In this study, bacterial community in the waste mining ponds of Balıkesir-Bigadiç and Eskişehir-Kırka Boron Mining Operations were investigated by using 16S rDNA gene-targeted Illumina MiSeq sequencing. The greater parts of high-throughput sequences were related to Proteobacteria, Planctomycetes, Bacteriodetes, Verrucomicrobia and Actinobacteria phyla. Cyanobacteria and Parcubacteria were other notable groups with low abundance. Genera belonged to Blastopirellula, Luteolibacter, Porhyrobacter and Hydrogenophaga were the most abundant taxa for all the samples. Sandarakinorhabdus, Pseudoanabena, Roseinatronobacter and Pontimonas genera-affiliated reads were also detected at in the October samples. Striking seasonal variations were detected between samples in terms of the type and number of microbial populations.

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Salt tolerant organisms are increasingly being used for the industrial production of high-value biomolecules due to their better adaptability compared to mesophiles. Chromohalobacter canadensis is one of the early halophiles to show promising biotechnology potential, which has not been explored to date.Advanced high throughput technologies such as whole-genome sequencing allow in-depth insight into the potential of organisms while at the frontiers of systems biology. At the same time, genome-scale metabolic models (GEMs) enable phenotype predictions through a mechanistic representation of metabolism. Here, we sequence and analyze the genome of C. canadensis 85B, and we use it to reconstruct a GEM. We then analyze the GEM using flux balance analysis and validate it against literature data on C. canadensis. We show that C. canadensis 85B is a metabolically versatile organism with many features for stress and osmotic adaptation. Pathways to produce ectoine and polyhydroxybutyrates were also predicted. The GEM reveals the ability to grow on several carbon sources in a minimal medium and reproduce osmoadaptation phenotypes. Overall, this study reveals insights from the genome of C. canadensis 85B, providing genomic data and a draft GEM that will serve as the first steps towards a better understanding of its metabolism, for novel applications in industrial biotechnology.

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