Levamisole promotes murine bone marrow derived dendritic cell activation and drives Th1 immune response in vitro and in vivo

Fu, Yubing; Wang, Ting; Xiu, Lei; Shi, Xiaojie; Bian, Ziyao; Zhang, Yongli; Ruhan, A; Wang, Xiao

doi:10.1016/j.intimp.2015.12.015

Cited by 24 publications

(16 citation statements)

References 44 publications

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“…IgE increased and was statistically significant (P<0.001). When LMS used with killed viruses can induce IgG production more rapidly and at higher levels, LMS injected with a DNA vaccine against the FMDV stimulated both humoral and cellular immune responses in conjunction with a strong production of IFN-γ, (Stelletta et al 2004;Kang et al 2005;Fu et al 2016). Levamisole and vitamin E administered groups resulted with high concentrations of IgM than control calves on day 22 and again increaselevel of IgG than that of control calves on day 1, 8, 15 and 22 of the trial (Pekmezci and Cakiroglu, 2009).…”

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confidence: 99%

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The Effect of Used Immunostimulating Drugs with FMD Vaccine on Immunoglobulins in Sheep

Abdullah¹,

Başbuğan

2020

Van Veterinary Journal

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In this study, the effect of used immunostimulating drugs with FMD vaccine on immunoglobulins was aimed to investigate in sheep. For this purpose 40 healthy sheep were divided into 4 groups. Blood and serum samples were taken from all animals from 0 days before the study. Blood samples were measured immediately, and IgA, IgG, IgM, IgE and Zn values from biochemical parameters were stored for measurement. Group I was applied only FMD vaccine, group II Levamisole with the FMD vaccine, group III Zinc with FMD vaccine and the last group IV AD3E was applied with FMD vaccine. After 21 days, blood and serum samples from all groups were taken again and hematologic and IgA, IgG, IgM, IgE and Zn values were compared according to the values of day 0. No statistical difference was found between the groups except for PLT in hematological parameters (p> 0, 05) when compared to pre-vaccination and post-vaccination. In groups II and III there was a significant increase in PLT levels. Biochemical analysis showed IgA, IgG, IgM and IgE levels to be higher in IgG (p <0, 05) and IgE (p <0,001) in groups II and III when levels compared to before vaccination. There were statistically significant increases in IgM (p <0.05) in group IV.

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confidence: 99%

“…According to Stelletta et al (2004), Kang et al(2005), Ocal et al (2007), Pekmezci and Cakiroglu (2009), Sadigh-Eteghad et al 2011and Fu et al (2016), LMS enhances immune system and increases level of the IgG significantly but increased level of IgE might be due to FMD vaccine since it contains adjuvant.…”

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confidence: 99%

The Effect of Used Immunostimulating Drugs with FMD Vaccine on Immunoglobulins in Sheep

Abdullah¹,

Başbuğan

2020

Van Veterinary Journal

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“…Levamisole acts on different immune cell populations and appears to restore depressed immune function rather than to suppress normal immune systems. It can stimulate the formation of antibodies to various antigens, enhance T-cell activation and proliferation, and potentiate monocyte and macrophage functions including phagocytosis and chemotaxis, with a preferential effect on T helper-1 cells and subsequent upregulation of interleukin-2, interleukin-12, and interferon-g [19][20][21]. The restoration of cellmediated immunity with alteration in HSV-specific lymphocyte transformation and cytokine production by levamisole had been observed in vitro, which can explain the clinical improvement of recurrent HSV infection after levamisole treatment [8].…”

Section: Discussionmentioning

confidence: 99%

“…The effect of levamisole on ameliorating the delayed inflammatory amplification by auto-reactive T lymphocytes in EMM are less clear. Some researchers have suggested that this outcome could be attributed to its effect on the sequestration or elimination of persistent antigen, increasing interleukin-2 production by T lymphocytes and increasing cytokine production in regulatory T lymphocytes [19][20][21][22].…”

Section: Discussionmentioning

confidence: 99%

The effect of levamisole in the treatment of recalcitrant recurrent erythema multiforme major: An observational study

Ren-feng¹,

Chen²,

Hui³

et al. 2018

Journal of Dermatological Science

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“…These phenotypic changes optimize conditions for T lymphocyte activation and differentiation ( 11 , 12 ). Since mature DCs potently stimulate adaptive immunity better than immature DCs, many vaccine adjuvants currently under development are designed to efficiently induce functional maturation and activation of DCs ( 13 – 15 ). In the present study, we investigated immunological function of HP-β-CD by determining its ability to mature and activate DCs leading to the induction of adaptive immunity.…”

Section: Introductionmentioning

confidence: 99%

Induction of Dendritic Cell Maturation and Activation by a Potential Adjuvant, 2-Hydroxypropyl-β-Cyclodextrin

2016

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2-Hydroxypropyl-β-cyclodextrin (HP-β-CD) is a chemically modified cyclic oligosaccharide produced from starch that is commonly used as an excipient. Although HP-β-CD has been suggested as a potential adjuvant for vaccines, its immunological properties and mechanism of action have yet to be characterized. In the present study, we investigated the maturation and activation of human dendritic cells (DCs) treated with HP-β-CD. We found that DCs stimulated with HP-β-CD exhibited a remarkable upregulation of costimulatory molecules, MHC proteins, and PD-L1/L2. In addition, the production of cytokines, such as TNF-α, IL-6, and IL-10, was modestly increased in DCs when treated with HP-β-CD. Furthermore, HP-β-CD-sensitized DCs markedly induced the proliferation and activation of autologous T lymphocytes. HP-β-CD also induced a lipid raft formation in DCs. In contrast, filipin, a lipid raft inhibitor, attenuated HP-β-CD-induced DC maturation, the cytokine expression, and the T lymphocyte-stimulating activities. To determine the in vivo relevance of the results, we investigated the adjuvanticity of HP-β-CD and the modulation of DCs in a mouse footpad immunization model. When mice were immunized with ovalbumin in the presence of HP-β-CD through a hind footpad, serum ovalbumin-specific antibodies were markedly elevated. Concomitantly, DC populations expressing CD11c and MHC class II were increased in the draining lymph nodes, and the expression of costimulatory molecules was upregulated. Collectively, our data suggest that HP-β-CD induces phenotypic and functional maturation of DCs mainly mediated through lipid raft formation, which might mediate the adjuvanticity of HP-β-CD.

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Levamisole promotes murine bone marrow derived dendritic cell activation and drives Th1 immune response in vitro and in vivo

Cited by 24 publications

References 44 publications

The Effect of Used Immunostimulating Drugs with FMD Vaccine on Immunoglobulins in Sheep

The Effect of Used Immunostimulating Drugs with FMD Vaccine on Immunoglobulins in Sheep

The effect of levamisole in the treatment of recalcitrant recurrent erythema multiforme major: An observational study

Induction of Dendritic Cell Maturation and Activation by a Potential Adjuvant, 2-Hydroxypropyl-β-Cyclodextrin

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