Leukocyte subpopulations in kidney and trachea of chickens infected with infectious bronchitis virus

Abstract

SUMMARYUsing immunohistochemical methods, we studied the nephropathogenicity of the infectious bronchitis virus (IBV)-strain V1648-and the leukocyte phenorypes in the pathological lesions in the kidneys and the trachea formed after inoculation with this virus strain. One-day-old WLA chickens were intravenously inoculated, and after 5, 7 and 11 days their kidneys, trachea and lungs were removed. Monoclonal antibodies were used to detect viral antigen, and lymphoid and non-lymphoid cell populations. In serial sections, the detection of the viral antigen was correlated to the phenotypes of the cells. At days 5 and 7 after inoculation, viral antigen was detected in the epithelium and the interstitium of the kidney tubuli and in the epithelium of the trachea. The infiltrated cells in these tissues were mainly of the T cell phenotype. The cellular immune reaction was correlated with the detection of viral antigen.

Prevalence, Serodiagnosis and Histopathological Changes in Field Cases of Infectious Bronchitis in Chickens

“…If air sac involvement occurs, there is edema, epithelial cell desquamation, and some fibrinous exudates within 24 hrs (Riddell, 1987). Janse et al, (1994) also reported the comparable findings like deciliation, sqamous metaplasia, cellular infiltration, edema of mucous and exudates as noticed by other workers (Butcher et al, 1990;Mahdavi et al, 2007). The virus replicates in ciliated epithelial cells causing deciliation, odema, desquamation, hyperplasia and mononuclear cell infiltration.…”

Detection of infectious bronchitis virus

“…The performance of the IPA in detecting IBV antigen after inoculation of SPF chickens compares very favourably with IFA (Nakamura et al, 1991;Owen et al, 1991;Janse et al, 1994;Chen et al, 1996). Naqi (1990) showed that IBV could be detected by IPA in the CAM of inoculated eggs within 15 h p.i., with a detection limit of 10 6.2 EID 50 .…”

Infectious bronchitis virus S1 gene sequence comparison is a better predictor of challenge of immunity in chickens than serotyping by virus neutralization

“…IBV strains that have been shown to be of different serotypes by VN may be able to induce partial or complete immunity in chickens (Hitchner et al ., 1964;Raggi & Lee, 1965;Winterfield et al ., 1972;Darbyshire, 1980Darbyshire, , 1985Arvidson et al ., 1990). The broader degree of cross-protection in birds is likely to be related to the cellmediated immune responses to shared T-cell epitopes among some heterologous strains (Janse et al ., 1994;Dhinakar Raj & Jones, 1997;Collisson et al ., 2000;Seo et al ., 2000).…”