Leukocyte-Mimicking Stem Cell Delivery via in Situ Coating of Cells with a Bioactive Hyperbranched Polyglycerol

Jeong, Jae Hyun; Schmidt, John J.; Kohman, Richie E.; Zill, Andrew T.; DeVolder, Ross J.; Smith, Clint; Lai, Mei Hsiu; Shkumatov, Artem; Jensen, Tor; Schook, Lawrence B.; Zimmerman, Steven C.; Kong, Hyunjoon

doi:10.1021/ja400636d

Cited by 75 publications

(66 citation statements)

References 32 publications

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“…The larger surface area is presents a more peptides or antibodies on the surfaces than the spherical ones. [69] As such, the tubular shaped polymersomes conjugated with cell adhesion peptides exhibit the increased adhesion to cell surfaces. Tubular polymersomes can be obtained using the film rehydration method where a thin film of amphiphilic copolymer is solvent-casted and placed in contact with an aqueous solution under stirring.…”

Section: Controlling Polymersome Shapementioning

confidence: 99%

Engineering Polymersomes for Diagnostics and Therapy

Leong

Teo

Aakalu

et al. 2018

Adv Healthcare Materials

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104

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Engineered polymer vesicles, termed as polymersomes, confer a flexibility to control their structure, properties, and functionality. Self-assembly of amphiphilic copolymers leads to vesicles consisting of a hydrophobic bilayer membrane and hydrophilic core, each of which is loaded with a wide array of small and large molecules of interests. As such, polymersomes are increasingly being studied as carriers of imaging probes and therapeutic drugs. Effective delivery of polymersomes necessitates careful design of polymersomes. Therefore, this review article discusses the design strategies of polymersomes developed for enhanced transport and efficacy of imaging probes and therapeutic drugs. In particular, the article focuses on overviewing technologies to regulate the size, structure, shape, surface activity, and stimuli- responsiveness of polymersomes and discussing the extent to which these properties and structure of polymersomes influence the efficacy of cargo molecules. Taken together with future considerations, this article will serve to improve the controllability of polymersome functions and accelerate the use of polymersomes in biomedical applications.

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Section: Controlling Polymersome Shapementioning

confidence: 99%

Engineering Polymersomes for Diagnostics and Therapy

Leong

Teo

Aakalu

et al. 2018

Adv Healthcare Materials

Self Cite

104

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“…The functionalized cells have been widely studied for applications such as immunotherapy, tissue engineering and stem cell homing. [1–9] These prominent methods are historically developed for unimolecular functionalization, i.e. , the display of single biomolecules on each spot of the cell surface.…”

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confidence: 99%

Polyvalent Display of Biomolecules on Live Cells

et al. 2018

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Surface display of biomolecules on live cells offers new opportunities to treat human diseases and perform basic studies. Existing methods are primarily focused on monovalent functionalization, that is, the display of single biomolecules across the cell surface. Here we show that the surface of live cells can be functionalized to display polyvalent biomolecular structures through two-step reactions under physiological conditions. This polyvalent functionalization enables the cell surface to recognize the microenvironment one order of magnitude more effectively than with monovalent functionalization. Thus, polyvalent display of biomolecules on live cells holds great potential for various biological and biomedical applications.

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“…These same principles have been utilized for cellular engineering, where the desired ligand or chemical moiety is conjugated to a hydrophobic anchor and then incubated with the to-be-labeled cell population. As a thermodynamically favored process (Fernandez-Vidal et al, 2011; Gorfe et al, 2008; Jeong et al, 2013), the hydrophobic anchor will spontaneously insert into the lipid bilayer and effectively tether the attached species to the membrane (Figure 1A). …”

Section: Non-genetic Approaches To Membrane Engineeringmentioning

confidence: 99%

“…Such membrane insertion has been accomplished with a variety of hydrophobic tags, including alkyl chains (Jeong et al, 2013), lipid moieties (Kean et al, 2012), and glycophosphatidylinositol (GPI) anchors (Hamdy et al, 2005). Multiple methods for attaching these tags to the desired cargo have also been developed.…”

Section: Non-genetic Approaches To Membrane Engineeringmentioning

confidence: 99%

“…Oligonucleotides have been installed as well, enabling the clustering of cells functionalized with complimentary sequences (Selden et al, 2012; Todhunter et al, 2015). Finally, some have attached larger cell-targeting scaffolds, including nanoparticles (Csizmar et al, 2018; Gabrielse et al, 2014; Jeong et al, 2013) and polymers (Takeo et al, 2015). Notably, these methods were further developed into synthetic targeting systems used to direct artificial cell-cell interactions in vitro .…”

Section: Non-genetic Approaches To Membrane Engineeringmentioning

confidence: 99%

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Programming Cell-Cell Interactions through Non-genetic Membrane Engineering

Csizmar

Petersburg

Wagner

2018

Cell Chemical Biology

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The ability to direct targeted intercellular interactions has the potential to enable and expand the use of cell-based therapies for regenerative medicine, tissue engineering, and immunotherapy. While genetic engineering approaches have proven effective, these techniques are not amenable to all cell types and often yield permanent modifications with potentially long-lasting adverse effects, restricting their application. To circumvent these limitations, there is intense interest in developing non-genetic methods to modify cell membranes with functional groups that will enable the recognition of target cells. While many such techniques have been developed, relatively few have been applied to directing specific cell-cell interactions. This review details these non-genetic membrane engineering approaches-namely, hydrophobic membrane insertion, chemical modification, liposome fusion, metabolic engineering, and enzymatic remodeling-and summarizes their major applications. Based on this analysis, perspective is provided on the ideal features of these systems with an emphasis on the potential for clinical translation.

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Leukocyte-Mimicking Stem Cell Delivery via in Situ Coating of Cells with a Bioactive Hyperbranched Polyglycerol

Cited by 75 publications

References 32 publications

Engineering Polymersomes for Diagnostics and Therapy

Engineering Polymersomes for Diagnostics and Therapy

Polyvalent Display of Biomolecules on Live Cells

Programming Cell-Cell Interactions through Non-genetic Membrane Engineering

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