Polyvalent Display of Biomolecules on Live Cells

Shi, Peng; Zhao, Nan; Lai, Jinping; Coyne, James; Gaddes, Erin R.; Wang, Yong

doi:10.1002/anie.201712596

Cited by 55 publications

(65 citation statements)

References 45 publications

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“…[197][198][199] Alternatively, DNA amphiphiles can be used to regulate cell-to-cell interactions through the anchoring of hydrophobic moieties and molecular hybridization of DNA. [200][201][202] In another work, Park et al reported that two amphiphilic block copolymers, poly(butadiene)-block-poly(ethylene oxide) (PBD-b-PEO) and polymethyl acrylate-block-DNA (PMA-b-DNA), could coassemble to form giant polymersomes ( Figure 20D); when two of this kind of polymersomes interacted via strand-hybridization, the DNA that originally uniformly distributed on the polymersome would gather to one side and form a DNA island. [195]…”

Section: Vesicle-dna Amphiphile Complexmentioning

confidence: 99%

Hydrophobic Interaction: A Promising Driving Force for the Biomedical Applications of Nucleic Acids

et al. 2020

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The comprehensive understanding and proper use of supramolecular interactions have become critical for the development of functional materials, and so is the biomedical application of nucleic acids (NAs). Relatively rare attention has been paid to hydrophobic interaction compared with hydrogen bonding and electrostatic interaction of NAs. However, hydrophobic interaction shows some unique properties, such as high tunability for application interest, minimal effect on NA functionality, and sensitivity to external stimuli. Therefore, the widespread use of hydrophobic interaction has promoted the evolution of NA-based biomaterials in higher-order self-assembly, drug/gene-delivery systems, and stimuli-responsive systems. Herein, the recent progress of NA-based biomaterials whose fabrications or properties are highly determined by hydrophobic interactions is summarized. 1) The hydrophobic interaction of NA itself comes from the accumulation of base-stacking forces, by which the NAs with certain base compositions and chain lengths show properties similar to thermal-responsive polymers. 2) In conjugation with hydrophobic molecules, NA amphiphiles show interesting self-assembly structures with unique properties in many new biosensing and therapeutic strategies. 3) The working-mechanisms of some NA-based complex materials are also dependent on hydrophobic interactions. Moreover, in recent attempts, NA amphiphiles have been applied in organizing macroscopic self-assembly of DNA origami and controlling the cell-cell interactions.

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Section: Vesicle-dna Amphiphile Complexmentioning

confidence: 99%

Hydrophobic Interaction: A Promising Driving Force for the Biomedical Applications of Nucleic Acids

et al. 2020

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“…S1A †). [43][44][45] In order to restore more anchor ss-DNAs on cell surfaces, chlorpromazine (CPZ) was added into the medium to inhibit endocytosis (the cytotoxicity of CPZ toward Jurkat cells was also tested as shown in Fig. S1B †).…”

Section: Resultsmentioning

confidence: 99%

DNA nanotweezers for stabilizing and dynamically lighting up a lipid raft on living cell membranes and the activation of T cells

Sun

Wang

et al. 2020

Chem. Sci.

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“…36 Recently, metabolic glycoengineering was used to label cell surface carbohydrates with DNA. 33,34 Lovendahl et al presented HUH-endonuclease domain fusion to covalently link DNA to proteins. 36 We attribute this rather scarce literature precedence to a lack of robust methods that allow conjugation without detriment to the protein function.…”

Section: Discussionmentioning

confidence: 99%

“…Metabolic glyco engineering 31,32 has been used to establish a covalent linkage between DNA and proteins on living cells, but this method does not allow tagging of specific proteins. 33,34…”

Section: Main Textmentioning

confidence: 99%

Live cell PNA labelling enables erasable fluorescence imaging of membrane proteins

Gavins

Gröger

Bartoschek

et al. 2020

Preprint

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DNA nanotechnology is an emerging field, which promises fascinating opportunities for the manipulation and imaging of proteins on a cell surface. The key to progress in the area is the ability to create the nucleic acid-protein junction in the context of living cells. Here we report a covalent labelling reaction, which installs a biostable peptide nucleic acid (PNA) tag. The reaction proceeds within minutes and is specific for proteins carrying a 2 kDa coiled coil peptide tag. Once installed the PNA label serves as a generic landing platform that enables the recruitment of fluorescent dyes via nucleic acid hybridization. We demonstrate the versatility of this approach by recruiting different fluorophores, assembling multiple fluorophores for increased brightness, and achieving reversible labelling by way of toehold mediated strand displacement. Additionally, we show that labelling can be carried out using two different coiled coil systems, with EGFR and ETBR, on both HEK293 and CHO cells. Finally, we apply the method to monitor internalization of EGFR on CHO cells.

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Polyvalent Display of Biomolecules on Live Cells

Cited by 55 publications

References 45 publications

Hydrophobic Interaction: A Promising Driving Force for the Biomedical Applications of Nucleic Acids

Hydrophobic Interaction: A Promising Driving Force for the Biomedical Applications of Nucleic Acids

DNA nanotweezers for stabilizing and dynamically lighting up a lipid raft on living cell membranes and the activation of T cells

Live cell PNA labelling enables erasable fluorescence imaging of membrane proteins

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