Leukocyte and Platelet Collection from Normal Donors with the Continuous Flow Blood Cell Separator

Abstract

Continuous‐flow centrifugation of blood from normal donors with the NCI‐IBM Continuous Flow Blood Cell Separator can be used safely and effectively to collect large quantities of normal granulocytes, lymphocytes, and platelets. Comparison with single‐unit leukapheresis demonstrated a threefold greater collection of granulocytes and a fivefold greater collection of lymphocytes per hour of leukapheresis with continuous flow centrifugation. Granulocyte collections can be enhanced by giving the donors etiocholanolone or cortisone prior to leukapheresis. Plateletpheresis can be combined efficiently with granulocyte collction during continuous‐flow centrifugation, but it offers no particular advantage over platelet collection by standard plasmapheresis technics.

Intravascular Fate of Granulocytes Administered by Granulocyte Transfusions

“…Similar half-disappearance times were observed when allogenic neutrophils were transfused to neutropenic recipients with infections [4]. The results of our cell kinetic studies in men are basically in accordance with similar observations in dogs obtained by Buckner et al [2], (5) In hematologically normal recipients compatible granulocytes ad ministered with granulocyte transfusions circulate for at least 20 h.…”

“…The difference may arise from cell interactions with the nylon fibers of the filters during cell sepa-ration. Margination of transfused granulocytes is probably the explana tion for the low recovery rates reported in the literature [5,6].…”

“…In others, the curves followed a decaying exponential component, indicat ing a random transit of transfused granulocytes from the vessels into the tissue. In these latter cases the half-disappearance times were either in the range of normal granulocytes or were prolonged (normal range of the method: 6.1-10.5 h; mean 7.6 h [5]). Similar half-disappearance times were observed when allogenic neutrophils were transfused to neutropenic recipients with infections [4].…”

Long-Term Follow-Up of Donors Cytapheresed More Than 50 Times

“…The intra vascular granulocyte pool represents only one fiftieth of the total extravascular com partment [19], The extravascular cells ap pear to be able to enter the circulation rap idly and immediately replace cells collected during leukapheresis. Donor granulocyte counts following continuous flow centrifu gation leukapheresis remain unchanged compared to predonation counts [20][21][22] and are actually increased after filtration leukapheresis [5,23]. In contrast, the reserve of mature platelets is limited, so that a mod est decrease in total platelet count occurs after the first 6-8 procedures collected on alternate days, but by the 10th procedure it has returned to baseline, presumably as a result of increased bone marrow production [24],…”

Granulocyte Transfusion Therapy of Experimental Pseudomonas Pneumonia

“…They were chosen because of previous experience with the effects of total body irradiation on these dogs (10) and because dogs of this size could be easily handled. The dogs used as leukocyte and platelet donors were English-American foxhounds, weighing [20][21][22][23][24][25][26][27][28][29][30] kg. These animals, entirely unrelated to beagles, were chosen as blood product donors because of their large size and docile temperament.…”