Granulocyte Transfusion Therapy of Experimental Pseudomonas Pneumonia

Revisiting the Use of Granulocyte Transfusions in Pediatric Oncology Patients

“…In the 1970s, GTFs were demonstrated to improve survival in neutropenic dog models of pseudomonal pneumonia, 3,9 and the use of GTF in human patients was accepted initially with a large degree of interest.…”

“…2 Notably, mortality rate has been linked to the degree and duration of neutropenia. 3,4 Granulocyte transfusions (GTFs) have had a controversial role in treating patients with fever and neutropenia. Initially, enthusiasm led some physicians in the 1970s to declare that withholding GTF from such patients was unethical.…”

In canine bacterial pneumonia circulating granulocyte counts determine outcome from donor cells

“…61,62 Transfusion of therapeutic granulocytes also has been shown to have survival benefits in studies of children and infants with neutropenia induced by either sepsis or chemotherapy, [63][64][65][66][67][68] while other studies found no benefit but also no evidence of harm. [69][70][71][72][73] There is also evidence in neutropenic septic canine models that granulocyte transfusions improve mortality, [74][75][76][77][78] shorten time to clearance of the offending microorganism from the bloodstream and tissues, [79][80][81][82] and decrease the risks of developing infection. 83 Our data are the first to evaluate therapeutic granulocyte transfusions in nonneutropenic subjects unable to expand circulating counts challenged with a severe infectious stimulus.…”

Pharmacology of Antibiotics

“…In an attempt to more clearly define the kinetics of antibiotic penetration, accumulation and clearance in bronchial secretions, an animal model was created (Pennington and Reynolds (1973 a&b); Pennington and Reynolds 1975 ); Pennington et al 1974, American foxhound dogs were anaesthetized with 2.5% sodium thiamylal and intubated with a single-cuff rubber endotracheal tube of 1 cm. internal diameter.…”