Leukocyte activation after coronary stenting in patients during the subacute phase of a previous ST-elevation myocardial infarction

Stakos, Dimitrios; Kotsianidis, Ιoannis; Tziakas, Dimitrios; Spanoudakis, Emmanuil; Chalikias, Georgios K.; Bourikas, Georgios; Katsaris, Georgios; Boudoulas, Harisios

doi:10.1097/mca.0b013e328011c094

Cited by 6 publications

(3 citation statements)

References 27 publications

(23 reference statements)

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“…We, however, have demonstrated increased monocyte activation as determined by increased binding of F7 to monocytes in STEMI patients within 36 h of PCI. Our results are in line with data from other groups which have detected increased monocyte activation after myocardial infarction by different means [66].…”

Section: Discussionsupporting

confidence: 93%

A DARPin targeting activated Mac-1 is a novel diagnostic tool and potential anti-inflammatory agent in myocarditis, sepsis and myocardial infarction

et al. 2021

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The monocyte β2-integrin Mac-1 is crucial for leukocyte–endothelium interaction, rendering it an attractive therapeutic target for acute and chronic inflammation. Using phage display, a Designed-Ankyrin-Repeat-Protein (DARPin) was selected as a novel binding protein targeting and blocking the αM I-domain, an activation-specific epitope of Mac-1. This DARPin, named F7, specifically binds to activated Mac-1 on mouse and human monocytes as determined by flow cytometry. Homology modelling and docking studies defined distinct interaction sites which were verified by mutagenesis. Intravital microscopy showed reduced leukocyte–endothelium adhesion in mice treated with this DARPin. Using mouse models of sepsis, myocarditis and ischaemia/reperfusion injury, we demonstrate therapeutic anti-inflammatory effects. Finally, the activated Mac-1-specific DARPin is established as a tool to detect monocyte activation in patients receiving extra-corporeal membrane oxygenation, as well as suffering from sepsis and ST-elevation myocardial infarction. The activated Mac-1-specific DARPin F7 binds preferentially to activated monocytes, detects inflammation in critically ill patients, and inhibits monocyte and neutrophil function as an efficient new anti-inflammatory agent.

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Section: Discussionsupporting

confidence: 93%

A DARPin targeting activated Mac-1 is a novel diagnostic tool and potential anti-inflammatory agent in myocarditis, sepsis and myocardial infarction

et al. 2021

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“…In our study, the results of CD64 on monocytes and neutrophils were similar at the time of suspected sepsis, but 24 hours later the diagnostic accuracy of CD64 on monocytes was significantly lower than on neutrophils. This data could indicate that activation in monocytes is faster or more rapidly completed than in neutrophils [32]. …”

Section: Discussionmentioning

confidence: 99%

Neutrophil and Monocyte CD64 and CD163 Expression in Critically Ill Neonates and Children with Sepsis: Comparison of Fluorescence Intensities and Calculated Indexes

Groselj-Grenc

Ihan

Derganc

2008

Mediators of Inflammation

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Objective. To evaluate the expression of CD64 and CD163 on neutrophils and monocytes in SIRS with/without sepsis and to compare the diagnostic accuracy of CD64 and CD163 molecules expression determined as (1) mean fluorescence intensities (MFI) of CD64 and CD163; and (2) the ratio (index) of linearized MFI to the fluorescence signal of standardized beads. Patients and methods. Fifty-six critically ill neonates and children with systemic inflammatory response syndrome (SIRS) and suspected sepsis, classified into two groups: SIRS with sepsis (n = 29) and SIRS without sepsis (n = 27). Results. CD64 and CD163 MFI measured on neutrophils and monocytes were elevated in patients with SIRS with sepsis. Diagnostic accuracy of indexes was equal to diagnostic accuracy of MFI for CD64 on neutrophils (0.833 versus 0.854 for day 0 and 0.975 versus 0.983 for day 1) and monocytes (0.811 versus 0.865 for day 0 and 0.825 versus 0.858 for day 1), and CD163 on neutrophils (0.595 versus 0.655 for day 0 and 0.677 versus 0.750 for day 1), but not for CD163 on monocytes. Conclusion. CD64 MFI, CD163 MFI, CD64 indexes for neutrophils and monocytes, and CD163 index for neutrophils can all be used for discrimination of SIRS and sepsis in critically ill neonates and children. CD64 index for neutrophils, however, is superior to all other markers.

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“…CD45 + EV are released from activated leukocytes (70) and laboratory investigations indicate that coronary stents can activate leukocytes (71). Moreover, clinical studies have reported leukocyte activation in patients receiving coronary stents (72). Hence, leukocyte activation triggered by placement of coronary stents may be the mechanism behind the observed correlation between the number of coronary stents placed and increasing levels of leukocyte EV.…”

Section: Discussionmentioning

confidence: 99%

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Leukocyte activation after coronary stenting in patients during the subacute phase of a previous ST-elevation myocardial infarction

Cited by 6 publications

References 27 publications

A DARPin targeting activated Mac-1 is a novel diagnostic tool and potential anti-inflammatory agent in myocarditis, sepsis and myocardial infarction

A DARPin targeting activated Mac-1 is a novel diagnostic tool and potential anti-inflammatory agent in myocarditis, sepsis and myocardial infarction

Neutrophil and Monocyte CD64 and CD163 Expression in Critically Ill Neonates and Children with Sepsis: Comparison of Fluorescence Intensities and Calculated Indexes

Data_Sheet_1.pdf

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