Neutrophil and Monocyte CD64 and CD163 Expression in Critically Ill Neonates and Children with Sepsis: Comparison of Fluorescence Intensities and Calculated Indexes

Abstract

Objective. To evaluate the expression of CD64 and CD163 on neutrophils and monocytes in SIRS with/without sepsis and to compare the diagnostic accuracy of CD64 and CD163 molecules expression determined as (1) mean fluorescence intensities (MFI) of CD64 and CD163; and (2) the ratio (index) of linearized MFI to the fluorescence signal of standardized beads. Patients and methods. Fifty-six critically ill neonates and children with systemic inflammatory response syndrome (SIRS) and suspected sepsis, classified into two groups: SIRS with sepsis (n = 29) and SIRS without sepsis (n = 27). Results. CD64 and CD163 MFI measured on neutrophils and monocytes were elevated in patients with SIRS with sepsis. Diagnostic accuracy of indexes was equal to diagnostic accuracy of MFI for CD64 on neutrophils (0.833 versus 0.854 for day 0 and 0.975 versus 0.983 for day 1) and monocytes (0.811 versus 0.865 for day 0 and 0.825 versus 0.858 for day 1), and CD163 on neutrophils (0.595 versus 0.655 for day 0 and 0.677 versus 0.750 for day 1), but not for CD163 on monocytes. Conclusion. CD64 MFI, CD163 MFI, CD64 indexes for neutrophils and monocytes, and CD163 index for neutrophils can all be used for discrimination of SIRS and sepsis in critically ill neonates and children. CD64 index for neutrophils, however, is superior to all other markers.

CD64 Index Provides Simple and Predictive Testing for Detection and Monitoring of Sepsis and Bacterial Infection in Hospital Patients

“…Several studies have indicated that the measurement of surface granulocyte CD64 may be useful in the detection of bacterial infection and sepsis in the workup of patients with systemic inflammatory response syndrome and fevers of unknown origin (1, 5, 9, 12-14, 17, 19-21, 23, 26, 30). Recently, two studies have demonstrated that CD64 measured as an index has high sensitivity and specificity for infection when used among neonatal intensive care unit patients (3,11). We set out to see whether we could confirm and extend these findings among an adult general hospital population.…”

“…Given the prior literature and two recent studies in pediatric populations, there is strong evidence that granulocyte CD64 expression represents a promising screening test for infection (3,6,8,11,14,20). Studies have compared patients with culture-proven infections and/or severe sepsis against control subjects in order to determine if antibiotics should be given to a patient or not.…”

Wnt5a Induces a Tolerogenic Phenotype of Macrophages in Sepsis and Breast Cancer Patients

“…6C, Supplemental Fig. 1E) (37,38). The reason for this discrepancy is not clear, but importantly, we could further show that Wnt5a/LPS treatment of ex vivo sepsis Mo lead to a prominent increase in CD163 expression (Fig.…”

Diagnostična vrednost označevalcev pri sepsi

“…do 1.000 molekul na celico nevtrofilca. V prisotnosti delov celične stene mikrobov, komplementa ali vnetnih citokinov (interferoni, granulocitno-makrofagne kolonije stimulirajoči faktor) se izraže-nost močno poveča (23). Ob odsotnosti stimulirajočih dejavnikov se izraženost zmanjša v 48 urah in normalizira v sedmih dneh (24).…”

“…Zanimala nas je zlasti uporabnost indeksa CD64 na nevtrofilcih. Ta označevalec so precej raziskovali za diagnosticiranje sepse pri otrocih (23,26) in pri razloče-vanju bolezni, ki jih klinično težko loči-mo med seboj, na primer sepso od težke okužbe brez sepse. Zato smo tudi mi preiskovance z okužbo na podlagi klinične slike in poteka ter laboratorijskih in mikrobioloških izvidov razdelili v skupini sepsa ter okužba brez sepse.…”