Leukemia inhibitory factor promotes porcine oocyte maturation and is accompanied by activation of signal transducer and activator of transcription 3

Dang‐Nguyen, Thanh Quang; Haraguchi, Satoru; Kikuchi, Kazuhiro; Somfai, T.; Bodó, Szilárd; Nagai, Takashi

doi:10.1002/mrd.22289

Cited by 34 publications

(31 citation statements)

References 49 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We speculate that FGF2 attenuates stress-induced cell damage, as observed for other cell types (58)(59)(60). LIF also may improve oocyte IVM in some species (61)(62)(63) through its ability to activate JAK-STAT3 and MAPK signaling pathways (61,62). The former pathway is crucial for maintaining CD9 expression in mouse oocytes (61,64).…”

Section: Discussionmentioning

confidence: 70%

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation

Yuan

Spate

Redel

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

110

115

View full text Add to dashboard Cite

Assisted reproductive technologies in all mammals are critically dependent on the quality of the oocytes used to produce embryos. For reasons not fully clear, oocytes matured in vitro tend to be much less competent to become fertilized, advance to the blastocyst stage, and give rise to live young than their in vivo-produced counterparts, particularly if they are derived from immature females. Here we show that a chemically defined maturation medium supplemented with three cytokines (FGF2, LIF, and IGF1) in combination, so-called "FLI medium," improves nuclear maturation of oocytes in cumulus-oocyte complexes derived from immature pig ovaries and provides a twofold increase in the efficiency of blastocyst production after in vitro fertilization. Transfer of such blastocysts to recipient females doubles mean litter size to about nine piglets per litter. Maturation of oocytes in FLI medium, therefore, effectively provides a fourfold increase in piglets born per oocyte collected. As they progress in culture, the FLI-matured cumulus-oocyte complexes display distinctly different kinetics of MAPK activation in the cumulus cells, much increased cumulus cell expansion, and an accelerated severance of cytoplasmic projections between the cumulus cells outside the zona pellucida and the oocyte within. These events likely underpin the improvement in oocyte quality achieved by using the FLI medium.cumulus cell | embryo development | genetic modification | in vitro fertilization | MAPK signaling

show abstract

Section: Discussionmentioning

confidence: 70%

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation

Yuan

Spate

Redel

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

110

115

View full text Add to dashboard Cite

show abstract

“…In sheep, oocytes cultured in presence of LIF showed higher in vitro fertilization and developmental potential (Ptak et al, ). A recent study has also uncovered a new role for LIF in porcine oocyte maturation (Dang‐Nguyen et al, ). Despite these beneficial phenotypes, the intracellular mechanism mediating this response is not clear.…”

Section: Introductionmentioning

confidence: 99%

Leukemia inhibitory factor enhances bovine oocyte maturation and early embryo development

Yuan

et al. 2014

Molecular Reproduction Devel

View full text Add to dashboard Cite

The present study was conducted to examine the effects of leukemia inhibitory factor (LIF) on bovine oocyte maturation and early embryo development in vitro. Results showed that LIF supplementation (25 ng/ml) enhanced nuclear maturation of intact cumulus-oocyte complexes (COCs) compared to the vehicle control. Similar results were observed in denuded oocytes, indicating that LIF directly influences oocyte development. LIF-treated oocytes showed a higher cortical-granule-migration rate and increased expression of CD9, a tetraspanin transmembrane protein essential for fertilization. After in vitro fertilization, oocytes receiving LIF supplementation exhibited a higher cleavage rate and yielded a significantly higher number of blastocysts. To further dissect the molecular mechanism underlying this LIF-induced bovine oocyte maturation phenotype, we examined the involvement of two signaling cascades, mitogen-activated protein kinases (MAPK3/1)- and the signal transducer and activator of transcription 3 (STAT3)-dependent pathways. Western blot results revealed that LIF phosphorylated MAPK3/1 and STAT3. Inhibition of MAPK3/1 activation with MEK inhibitor U0126 only partially blocked LIF-induced nuclear maturation, although it attenuated oocyte cytoplasmic maturation. Inhibition of JAK/STAT3 activation with a specific pharmacological inhibitor completely abolished the LIF-response in bovine oocyte. In summary, these data revealed a novel role for LIF in bovine oocyte maturation subsequent embryonic development.

show abstract

“…Maturation of mammalian oocytes is a complex physiological process involving a large number of regulatory factors, cell structures, and different signaling pathways (Ball et al, 2014;Dang-Nguyen et al, 2014;Prunskaite-Hyyryläinen et al, 2014). For example, the cyclic adenosine monophosphate (cAMP) signaling pathway is necessary for maintaining meiosis, whereas the MAPK signaling pathway plays an important role in meiosis initiation, spindle assembly, and M2 arrest.…”

Section: Discussionmentioning

confidence: 99%

Single cell transcriptome profiling revealed differences in gene expression during oocyte maturation in Haimen white goats

Yin¹,

Cheng²,

Guo³

et al. 2017

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Juvenile in vitro embryo transfer is an important animal reproductive technology that can shorten the generation interval of livestock, explore the reproductive potential of dams with excellent genetic traits, accelerate genetic progress and production efficiency of the herd, and provide a wealth of genetic resources for livestock breeding. However, oocytes from kids do not develop as well as those from female goats during in vitro maturation. To identify differences during different stages of oocyte maturation, we used single cell transcriptome sequencing to compare gene expression in mature oocytes from kids and female goats. We identified 1086 differentially expressed genes in mature oocytes from kids and female goats. Of these, we observed upregulated expression in 355 genes and downregulated expression in 435 genes. The differentially expressed genes were involved in a total of 245 different pathways; of which 30 were significant (P ≤ 0.05). We used real-time quantitative polymerase chain reaction to screen and verify the expression of five genes specifically involved in oocyte maturation (MOS, RPS6KA1, CPEB1, ANAPC13, and CDK1). Further study of these genes will be of great importance for improving the reproductive performance of Haimen white goats.

show abstract

Leukemia inhibitory factor promotes porcine oocyte maturation and is accompanied by activation of signal transducer and activator of transcription 3

Cited by 34 publications

References 49 publications

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation

Leukemia inhibitory factor enhances bovine oocyte maturation and early embryo development

Single cell transcriptome profiling revealed differences in gene expression during oocyte maturation in Haimen white goats

Contact Info

Product

Resources

About