Leukemia inhibitory factor and its receptor: expression and regulation in the porcine endometrium throughout the estrous cycle and pregnancy

Yoo, Inkyu; Chae, Soogil; Han, Jisoo; Lee, Soohyung; Kim, Hyun Jong; Ka, Hakhyun

doi:10.5713/ajas.18.0429

Cited by 12 publications

(15 citation statements)

References 32 publications

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“…Our results are in general agreement with these findings in endometrium from D24 over D18. LIF-R expression in pregnant sows during the implantation period reaches maximum rates around day 15 post-insemination to eventually decrease thereafter (21). Our results agree with this report, with LIF-R expression in both hemi-allogeneic and allogeneic groups being considerably reduced on D24 compared to D18.…”

Section: Discussionsupporting

confidence: 91%

“…This study describes the endometrial expression of LIF and its receptor during the implantation period in pigs bred by ET employing donor embryos, a situation not explored before, as far as we can document. Our results, considering the use of conventional AI, confirm previous reports (13,(19)(20)(21)28), indicating that both LIF and LIF-R are expressed in the pig endometrium during the implantation period of pregnancy.…”

Section: Discussionsupporting

confidence: 91%

“…The IL-6 family of cytokines (including LIF) shares a specific receptor called LIF-R (17), with recognized roles in reproductive processes (18). LIF and LIF-R expression have been previously reported in the porcine endometrium and placenta (13,(19)(20)(21). These authors described their expression patterns during the estrous cycle and pregnancies performed by AI but, to the best of our knowledge, there are not previous studies about LIF or LIF-R expression in pregnancies stablished after ET.…”

Section: Introductionmentioning

confidence: 99%

“…Recent advances in porcine ET technology have allowed its application in the porcine industry today (1). However, the reproductive performance of recipients after ET continue to be suboptimal, mostly due to the high embryo mortality depicted (>50%), compared to natural breeding or artificial insemination (AI) (4,5), particularly during the implantation period (Days [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30]. During this period, embryos are responsible of triggering the so-called maternal immune tolerance to the embryo and placental allografts (6).…”

Section: Introductionmentioning

confidence: 99%

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Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During Implantation

et al. 2020

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Despite its advantages for pig breeding, embryo transfer (ET) has a major handicap: high embryo mortality during the pre- and implantation period, probably caused by divergent phenomena of tolerance between the immunologically unrelated (i.e., allogeneic) embryos and the recipient sow. Thus, to reach a similar maternal tolerance as in conventional breeding by artificial insemination (AI) would be the key to ET-success. For this reason, we studied the expression of the leukemia inhibitory factor (LIF) cytokine and its receptor in the pig endometrium during the implantation period (days 18 and 24) in sows subjected to ET (AL group) vs. post-cervical-AI controls (Hemi-AL group). Quantification of expression was performed at both mRNA (rt-qPCR) and protein (WB) levels. The expression of endometrial LIF on day 24 was considerably lower in ET than in AI pregnancies. Correlations between endometrial mRNA levels of LIF and LIF-R showed that, contrary to early AI-pregnancies, ET-pregnancies lack an inverse relation between cytokine and receptor levels. In conclusion, ET-pregnancies lack sufficient endometrial levels of LIF to develop adequate immunotolerance mechanisms to prevent the rejection of allogeneic ET-embryos.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During Implantation

et al. 2020

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“…Successful implantation and pregnancy maintain depend on stromal cells decidualization which has been demonstrated to play important roles in reproductive disorders such as RIF [10] and recurrent miscarriage [11]. Mechanistically, leukemia inhibitory factor (LIF), a member of IL-6 family, plays a vital role in the progression of decidualization and embryo to implantation [12]. What's more important, LIF-mediated STAT3 activation by promoting the phosphorylation of STAT3 to regulate the dialogue between endometrium and embryo [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

MiR-133b Improves Decidualization of Endometrial Stromal Cells by Targeting KLF12 in Recurrent Implantation Failure

Mei

Kong

Wang

et al. 2021

Preprint

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Purpose Impaired decidualization contributes to the infertility in recurrent implantation failure (RIF). Herein, we focused on the function and probable mechanisms of miR-133b in endometrial stromal cells decidualization.Methods miR-133b and KLF12 protein levels in midsecretory endometrial tissues derived from women with and without RIF were measured by qRT-PCR and Western blot. Primary human endometrial stromal cells (HESCs) were isolated and cultured for in vitro decidualization assays. Luciferase reporter, qRT-PCR and Western blot assays were used to measure the relationship between miR-133b and KLF12.Results miR-133b was significantly downregulated, whereas KLF12 was upregulated in endometrial tissues from RIF. miR-133b effectively promoted HESCs in vitro decidualization through the modulation of KLF12 expression and the activation of LIF/STAT3 pathway. Conversely, inhibition of miR-133b expression reversed these effects. In addition, the luciferase reporter system demonstrated that miR-133b directly inhibited the expression of KLF12 by interacting with 3’ untranslated region of KLF12.Conclusion Our data suggest that miR-133b promotes HESCs decidualization by targeting KLF12 and reverses the impaired decidualization in RIF.

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Rapid generation of functional nanovesicles from human trophectodermal cells for embryo attachment and outgrowth

Poh,

Rai,

Pangestu

et al. 2023

Proteomics

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Extracellular vesicles (EVs) are important mediators of embryo attachment and outgrowth critical for successful implantation. While EVs have garnered immense interest in their therapeutic potential in assisted reproductive technology by improving implantation success, their large‐scale generation remains a major challenge. Here, we report a rapid and scalable production of nanovesicles (NVs) directly from human trophectoderm cells (hTSCs) via serial mechanical extrusion of cells; these NVs can be generated in approximately 6 h with a 20‐fold higher yield than EVs isolated from culture medium of the same number of cells. NVs display similar biophysical traits (morphologically intact, spherical, 90–130 nm) to EVs, and are laden with hallmark players of implantation that include cell‐matrix adhesion and extracellular matrix organisation proteins (ITGA2/V, ITGB1, MFGE8) and antioxidative regulators (PRDX1, SOD2). Functionally, NVs are readily taken up by low‐receptive endometrial HEC1A cells and reprogram their proteome towards a receptive phenotype that support hTSC spheroid attachment. Moreover, a single dose treatment with NVs significantly enhanced adhesion and spreading of mouse embryo trophoblast on fibronectin matrix. Thus, we demonstrate the functional potential of NVs in enhancing embryo implantation and highlight their rapid and scalable generation, amenable to clinical utility.

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Leukemia inhibitory factor and its receptor: expression and regulation in the porcine endometrium throughout the estrous cycle and pregnancy

Cited by 12 publications

References 32 publications

Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During Implantation

Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During Implantation

MiR-133b Improves Decidualization of Endometrial Stromal Cells by Targeting KLF12 in Recurrent Implantation Failure

Rapid generation of functional nanovesicles from human trophectodermal cells for embryo attachment and outgrowth

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