Leuconostoc mesenteroides fermentation produces butyric acid and mediates Ffar2 to regulate blood glucose and insulin in type 1 diabetic mice

Traisaeng, Supitchaya; Batsukh, Anir; Chuang, Tsung Hsien; Herr, Deron R.; Huang, Yu; Chimeddorj, Battogtokh; Huang, Chun Ming

doi:10.1038/s41598-020-64916-2

Cited by 33 publications

(38 citation statements)

References 66 publications

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“…When comparisons were made across L. mesenteroides and other isolates, the lowest Ca 2+ ions was release by L. mesenteroides . The results of high acid production but low tooth demineralization could be explained by the fact that some type of L. mesenteroides strain produce butyric acid instead of lactic acid, since there is lack of evidence to confirm the association between butyric acid and dental caries [ 46 ]. These results suggested that acid production and acid resistance alone could not determine the cariogenicity of a bacterium.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Potentially Pathogenic Microorganisms Associated with Dental Caries in Human Teeth Biofilms

et al. 2020

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Dental caries is attributed to the predominance of cariogenic microorganisms. Cariogenic microorganisms are pathological factors leading to acidification of the oral microenvironment, which is related to the initiation and progression of caries. The accepted cariogenic microorganism is Streptococcus mutans (S. mutans). However, studies have found that caries could occur in the absence of S. mutans. This study aimed to assess the presence of potentially cariogenic microorganisms in human teeth biofilm. The microorganisms were isolated from human mouth and freshly extracted human maxillary incisors extracted for reasons of caries. The isolates were sorted based on their acidogenic and aciduric properties, and the S. mutans was used as the reference strain. Four potentially cariogenic strains were selected. The selected strains were identified as Streptococcus salivarius (S. salivarius), Streptococcus anginosus (S. anginosus), Leuconostoc mesenteroides (L. mesenteroides), and Lactobacillus sakei (L. sakei) through morphological analysis followed by 16S rRNA gene sequence analysis. The cariogenicity of isolates was analyzed. We show, for the first time, an association between L. sakei (present in fermented food) and dental caries. The data provide useful information on the role of lactic acid bacteria from fermented foods and oral commensal streptococci in dental caries.

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Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Potentially Pathogenic Microorganisms Associated with Dental Caries in Human Teeth Biofilms

et al. 2020

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“…The aqueous phase was transferred to an autosampler vial and 100 μL of concentrated HCl were added. The analysis of butyric acid was performed using an Agilent 1200 series HPLC system with a ZORBAX Eclipse XDB-C18 column (4.6 × 250 mm, 5 μm) [ 16 ]. The mobile phase consisted of 20 mmol/L NaH 2 PO 4 solution (pH 2.2) and acetonitrile.…”

Section: Methodsmentioning

confidence: 99%

“…Mice were fasted for 4-8 h on the last day of treatment. The level of blood glucose from the tail blood was measured using a glucometer [16]. The abdominal fat mass was photographed and weighted.…”

Section: Feeding Mice With L Mesenteroides Eh-1 and Ffar2 Inhibitionmentioning

confidence: 99%

“…The data further confirmed our earlier results that glucose can trigger and enhance the fermentation of L. mesenteroides EH-1. We previously demonstrated that butyric acid was detectable in the culture media of L. mesenteroides EH-1 [16]. We next investigated whether the culture media can affect the formation of lipid droplets during the differentiation process of adipocytes.…”

Section: Reduction Of Lipid Droplets In Differentiated 3t3-l1 Adipocymentioning

confidence: 99%

“…We isolated a butyric acid-producing L. mesenteroides EH-1 strain from Mongolian curd cheese. Our previous study demonstrated that L. mesenteroides EH-1 can reduce blood glucose and increase the insulin in the model of type 1 diabetic mice induced by streptozotocin [ 16 ]. In this study, we fed mice with HFD to augment the abdominal fat depots and investigated whether Ffar2 mediates the regulation of L. mesenteroides EH-1 on accumulation of visceral fats.…”

Section: Introductionmentioning

confidence: 99%

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Mouse Abdominal Fat Depots Reduced by Butyric Acid-Producing Leuconostoc mesenteroides

et al. 2020

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The activation of peroxisome proliferator-activated rece ptor gamma (PPAR-γ) is known to induce the differentiation of adipocytes. This study aimed to investigate the probiotic effect of Leuconostoc mesenteroides (L. mesenteroides) on high-fat diet (HFD)-induced PPAR-γ activation and abdominal fat depots. Incubation of differentiated 3T3-L1 adipocytes with media of L. mesenteroides EH-1, a butyric acid-producing strain, significantly reduced the amounts of lipid droplets. The oral administration of L. mesenteroides EH-1 produced large amounts (>1 mM) of butyric acid in cecum and attenuated the HFD-induced upregulation of PPAR-γ and accumulation of abdominal fats in mice. The combination of 2% glucose with L. mesenteroides EH-1 increased the production of butyric acid and potentiated the probiotic activity of L. mesenteroides EH-1 against the formation of lipid droplets in 3T3-L1 adipocytes as well as abdominal fats in HFD-fed mice. The inhibition of free fatty acid receptor 2 (Ffar2) by its antagonist, GLPG-0974, markedly diminished the probiotic effects of L. mesenteroides EH-1 plus glucose on the suppression of HFD-induced PPAR-γ and abdominal fats. Besides demonstrating the probiotic value of L. mesenteroides EH-1, our results highlight the possible therapy targeting the butyric acid-activated Ffar2 pathway to reduce abdominal fats.

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Effect of the administration of probiotics on the fecal microbiota of adult individuals

Zhao

Jin

Shen

et al. 2021

Food Science & Nutrition

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Probiotics have been used to ameliorate ailments by modulating gut microbiota. However, to date, the effects of probiotic supplementation on the composition of fecal microbiota in healthy adults remain obscure. In this study, nine healthy volunteers were recruited to take probiotics (a mixture of Lactobacillus casei Zhang, L. plantarum P‐8, and Bifidobacterium lactis V9, 2:2:3, 1 × 1010 CFU/day) for 28 days. The fecal samples were collected at 0 and 28 days, and V4 of the 16S rRNA gene sequenced by Illumina MiSeq was used to analyze the fecal microbiota. The enterotype has been used to characterize the composition of gut microbiota. Nine adults were divided into Type P (fecal microbiota dominated by Prevotella, 4 adults) and Type B (fecal microbiota dominated by Bacteroides, 5 adults) based on an enterotype analysis. The responses of variation had been found in two enterotypes. The α‐diversity was not changed significantly after the administration of probiotics in both Type P and B. However, the community structure in Type B was substantially influenced. After the administration of probiotics, Weissella and Leuconostoc were significantly higher in Type P, while Collinsella significantly increased in Type B. The different pathways involving pathogen infections were downregulated at 28 days. The Type VI secretion system and the EHEC/EPEC pathogenicity signature were downregulated in Type B and Type P, respectively.

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Leuconostoc mesenteroides fermentation produces butyric acid and mediates Ffar2 to regulate blood glucose and insulin in type 1 diabetic mice

Cited by 33 publications

References 66 publications

Isolation and Identification of Potentially Pathogenic Microorganisms Associated with Dental Caries in Human Teeth Biofilms

Isolation and Identification of Potentially Pathogenic Microorganisms Associated with Dental Caries in Human Teeth Biofilms

Mouse Abdominal Fat Depots Reduced by Butyric Acid-Producing Leuconostoc mesenteroides

Effect of the administration of probiotics on the fecal microbiota of adult individuals

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