Leucodine blocks mitotic clonal expansion during preadipoctye differentiation through cell cycle arrest

Abood, Steven; Veisaga, Maria‐Luisa; López, Leonardo; Barbieri, M. Alejandro

doi:10.1016/j.phytol.2017.12.011

Cited by 2 publications

(5 citation statements)

References 41 publications

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“…A total of 13 different cell lines originated from humans, mice, and rats were evaluated in the studies ( Figure 3 ). Among them, four cells were cancer cell lines including, mice melanoma B16 cells ( Ye et al, 2011 ), human HL-60 cell line ( Choi et al, 2002 ; Kim et al, 2005 ; Kim et al, 2006 ; Kweon et al, 2015 ), human CML cell line K562 ( Huang et al, 2016 ; Cai et al, 2017 ), human AML cell line U937 ( Huang et al, 2016 ), three cells were stem cells including rat mesenchymal stem cell ( Li et al, 2012 ), mice mesenchymal stem cells C3H10T1/2 ( Kim et al, 2017 ), hematopoietic stem and progenitor cells (HSPCs) ( de Lichtervelde et al, 2013 ), and six cells were normal cell lines including, 3T3-L1 cell line ( Galvis et al, 2011 ; Jang, 2016 ; Kim et al, 2016 ; Abood et al, 2018 ; Kwak and Kim, 2019 ), mice bone marrow macrophage ( Cheon et al, 2014 ; Hu et al, 2019 ; Lee et al, 2019 ; Li et al, 2019 ; Zhou et al, 2019 ; Lu et al, 2020 ), CD4 + T cell ( Park et al, 2016 ), human periosteum-derived cells ( Park et al, 2020 ), human preadipocytes ( Jang, 2016 ), mice MC3T3-E1 cell line ( Kim et al, 2017 ). All cell lines except the murine melanoma B16 cell line had a mesodermal origin.…”

Section: Resultsmentioning

confidence: 99%

“…The phosphorylation of some components from the MAPK pathway, such as ERK, p38, and JNK, was evaluated during inducing or inhibiting differentiation via sesquiterpene lactones in some of the studies ( n = 10) included in this systematic review ( Table 1 ). The level of phosphorylation of these components was either increased ( Kim et al, 2005 ; Kweon et al, 2015 ), decreased ( Ye et al, 2011 ; Park et al, 2016 ; Abood et al, 2018 ; Zhou et al, 2019 ; Lu et al, 2020 ), or unchanged ( Galvis et al, 2011 ; Cheon et al, 2014 ; Park et al, 2016 ; Li et al, 2019 ; Lu et al, 2020 ); however, there was no association between the status of phosphorylation and induction or inhibition of differentiation. The expression of c-Fos downstream of the MAPK pathway is also affected by sesquiterpene lactones ( Cheon et al, 2014 ; Lu et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

“…Also, PI3K/AKT pathway can indirectly affect cellular differentiation by interfacing with other signaling pathways such as the NFκB pathway (through IKK activation) and JAK/STAT pathway ( Kanehisa, 2019 ; Kanehisa and Goto, 2000 ; Kanehisa et al, 2021 ). The involvement of the PI3K-AKT pathway in response to three sesquiterpene lactones (i.e., atractylenolide I and II, 11,13-dihydro-[11R]-dehydroleucodine, isoalantolactone) through AKT has been reported ( Ye et al, 2011 ; Abood et al, 2018 ; Lu et al, 2020 ). The level of phosphorylation of AKT was decreased in treatment with three lactones with either inhibiting or inducing effects ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…In one study, the expression of SHH and GLI was increased by atractrynolide with inducing effects on differentiation, indicating the possible involvement of Sonic-Hedgehog signaling pathway in the differentiation ( Li et al, 2012 ). Sesquiterpene lactones are also found to decrease the expression of Fas involved in apoptosis ( Jang, 2016 ; Abood et al, 2018 ), increase the phosphorylation and expression of PKC involved in signaling in general ( Kim et al, 2005 ) and increase the expression of p27 and p21 and decrease the expression of c-Myc involved in the cell cycle ( Choi et al, 2002 ; Abood et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

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The Effects of Sesquiterpene Lactones on the Differentiation of Human or Animal Cells Cultured In-Vitro: A Critical Systematic Review

Fateh

Shekari

et al. 2022

Front. Pharmacol.

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Cellular differentiation is pivotal in health and disease. Interfering with the process of differentiation, such as inhibiting the differentiation of adipocytes and inducing the differentiation of cancer cells, is considered a therapeutic approach. Sesquiterpene lactones, primarily found in plants, have been attracted attention as differentiating/dedifferentiating agents tested on various human or animal cells. However, a consensus on sesquiterpene lactones’ effects and their mechanism of action is required. In this sense, through a systematic review, we have investigated the differentiating/dedifferentiating effects of sesquiterpene lactones on human or animal cells. 13 different cell lines originated from humans, mice, and rats, in addition to the effects of a total of 21 sesquiterpene lactones, were evaluated in the included studies. These components had either inducing, inhibiting, or no effect on the cells, mediating their effects through JAK-STAT, PI3K-Akt, mitogen-activated protein kinases, NFκB, PPARγ pathways. Although nearly all inducing and inhibiting effects were attributed to cancerous and normal cells, respectively, this is likely a result of a biased study design. Few studies reported negative results along with others, and no study was found reporting only negative results. As a result, not only are the effects and mechanism of action of sesquiterpene lactones not vivid but our knowledge and decisions are also misconducted. Moreover, there is a significant knowledge gap regarding the type of evaluated cells, other sesquiterpene lactones, and the involved signaling pathways. In conclusion, sesquiterpene lactones possess significant effects on differentiation status, leading to potentially efficient therapy of obesity, osteoporosis, and cancer. However, reporting negative results and further investigations on other cells, sesquiterpene lactones, and signaling pathways are highly suggested to pave the path of sesquiterpene lactones to the clinic more consciously.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

The Effects of Sesquiterpene Lactones on the Differentiation of Human or Animal Cells Cultured In-Vitro: A Critical Systematic Review

Fateh

Shekari

et al. 2022

Front. Pharmacol.

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“…Similarly, other sesquiterpenoids were also found to be able to suppress MAPK activation. Abood et al [ 44 ] reported that leucodine inhibited adipogenesis and suppressed the PPARγ pathway in 3T3-L1 preadipocytes by blocking mitotic clonal expansion via diminished p27 expression as a result of suppressed activation of ERK MAPK. Ye et al [ 45 ] isolated sesquiterpenoids from Atractylodes macrocephala , which exerted anti-tumor properties by blocking the growth of B16 melanoma cells.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effects of Sesquiterpenoids Isolated from Artemisia scoparia on Adipogenic Differentiation of 3T3-L1 Preadipocytes

Lee,

Oh,

Karadeniz

et al. 2023

IJMS

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Obesity and related complications are significant health issues in modern society, largely attributed to a sedentary lifestyle and a carbohydrate-rich diet. Since anti-obesity drugs often come with severe side effects, preventative measures are being sought globally, including dietary changes and functional foods that can counteract weight gain. In this context, plant-based metabolites are extensively studied for their advantageous biological effects against obesity. Several plants within the Artemisia genus have been reported to possess anti-adipogenic properties, preventing adipocytes from maturing and accumulating lipids. The present study investigated the anti-adipogenic potential of two sesquiterpenoids, reynosin and santamarine, isolated from A. scoparia in adipose-induced 3T3-L1 preadipocytes. Differentiating 3T3-L1 adipocytes treated with these isolated compounds displayed fewer adipogenic characteristics compared to untreated mature adipocytes. The results indicated that cells treated with reynosin and santamarine accumulated 55.0% and 52.5% fewer intracellular lipids compared to untreated control adipocytes, respectively. Additionally, the mRNA expression of the key adipogenic marker, transcription factor PPARγ, was suppressed by 87.2% and 91.7% following 60 μM reynosin and santamarine treatment, respectively, in differentiated adipocytes. Protein expression was also suppressed in a similar manner, at 92.7% and 82.5% by 60 μM reynosin and santamarine treatment, respectively. Likewise, SERBP1c and C/EBPα were also downregulated at both gene and protein levels in adipocytes treated with samples during differentiation. Further analysis suggested that the anti-adipogenic effect of the compounds might be a result of AMPK activation and the subsequent suppression of MAPK phosphorylation. Overall, the present study suggested that sesquiterpenoids, reynosin, and santamarine were two potential bioactive compounds with anti-adipogenic properties. Further research is needed to explore other bioactive agents within A. scoparia and elucidate the in vivo action mechanisms of reynosin and santamarine.

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Leucodine blocks mitotic clonal expansion during preadipoctye differentiation through cell cycle arrest

Cited by 2 publications

References 41 publications

The Effects of Sesquiterpene Lactones on the Differentiation of Human or Animal Cells Cultured In-Vitro: A Critical Systematic Review

The Effects of Sesquiterpene Lactones on the Differentiation of Human or Animal Cells Cultured In-Vitro: A Critical Systematic Review

Inhibitory Effects of Sesquiterpenoids Isolated from Artemisia scoparia on Adipogenic Differentiation of 3T3-L1 Preadipocytes

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