Leucocytosis in the Mouse induced by Serial Blood Sampling from the Tail

Jyväsjärvi, Simo; Keinänen, Sirkka; Ruuskanen, Olli

doi:10.1038/230122b0

Cited by 6 publications

(2 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The analysis of PDT-induced blood neutrophilia in mice is evidently complicated by nonspecific stress effects (28,29) sustained through the handling of the animals during the experimental procedure. However, we have been able to clearly validate the extent of PDT-specific neutrophilia by deducting the nonspecific changes evidenced in light-onlytreatment groups.…”

Section: Discussionmentioning

confidence: 99%

“…2 cannot be attributed solely to the action of PDT. It is known that stress reactions cause changes in blood leukocyte levels, mainly through the induced release of adrenocortical hormones (28)(29)(30). The nonspecific stress provoked by restraining mice during the light treatment and also by repeated blood sampling ( Fig.…”

Section: Analysis Of Peripheral Blood Collected By Consecutive Samplimentioning

confidence: 99%

See 1 more Smart Citation

Induction of Systemic Neutrophil Response in Mice by Photodynamic Therapy of Solid Tumors¶

Cecić¹,

Parkins

Korbelik

2001

Photochem Photobiol

View full text Add to dashboard Cite

Photodynamic therapy (PDT) of solid tumors elicits a strong, acute inflammatory response characterized by a rapid and massive infiltration of activated neutrophils into the tumor. The present study investigated the impact of PDT on the systemic and local (treatment site) kinetics of neutrophil trafficking and activity in mouse SCCVII and EMT6 tumor models. Differential leukocyte counts in the peripheral blood of treated mice revealed a pronounced neutrophilia developing rapidly after Photofrin porfimer sodium (Photofrin)- or tetra(m-tetrahydroxyphenyl)chlorin (mTHPC)-based PDT. Significant neutrophilia was also observed upon PDT treatment of normal dorsal skin but not on the footpad of tumor-free mice. The changes in circulating neutrophil numbers were accompanied by an efflux of these cells from the bone marrow. An increased proportion of cells with high L-selectin (CD62L antigen) expression was found among bone-marrow-residing neutrophils 6-24 h after PDT, and in neutrophils in the peripheral circulation and treated tumors 24 h after therapy. Complement inhibition completely prevented the development of PDT-induced neutrophilia. The results of the present study demonstrate that treatment of solid tumors by PDT induces a strong and protracted increase in systemic neutrophil numbers mediated by complement activation. This reaction reflects rapid and massive mobilization and activation of neutrophils for the destruction of PDT-treated tumor tissue.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Analysis Of Peripheral Blood Collected By Consecutive Samplimentioning

confidence: 99%