Leucine-rich repeat receptor-like gene screen reveals that Nicotiana RXEG1 regulates glycoside hydrolase 12 MAMP detection

Wang, Yan; Xu, Yuanpeng; Sun, Yujing; Wang, Huibin; Qi, Jiaming; Wan, Bowen; Ye, Wenwu; Lin, Yachun; Shao, Yuanyuan; Dong, Suomeng; Tyler, Brett M.; Wang, Yuanchao

doi:10.1038/s41467-018-03010-8

Cited by 150 publications

(201 citation statements)

References 66 publications

(91 reference statements)

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“…, ; Wang et al. , ). Here, we show that the kinase activity of At SOBIR1 is essential for its phosphorylation.…”

Section: Discussionmentioning

confidence: 99%

“…, ; Wang et al. , ). Pre‐formation of immune complexes at nanoclusters at the PM, without their activation by the matching ligand, has been shown previously (Bücherl et al.…”

Section: Discussionmentioning

confidence: 99%

“…, ; Wang et al. , ). For example, BAK1 is specifically recruited to the Cf‐4/SOBIR1 complex on perception of Avr4 from C. fulvum by Cf‐4 (Postma et al.…”

Section: Introductionmentioning

confidence: 99%

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Kinase activity of SOBIR1 and BAK1 is required for immune signalling

Burgh

Postma

Robatzek

et al. 2019

Molecular Plant Pathology

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Summary Leucine‐rich repeat‐receptor‐like proteins (LRR‐RLPs) and LRR‐receptor‐like kinases (LRR‐RLKs) trigger immune signalling to promote plant resistance against pathogens. LRR‐RLPs lack an intracellular kinase domain, and several of these receptors have been shown to constitutively interact with the LRR‐RLK Suppressor of BIR1‐1/EVERSHED (SOBIR1/EVR) to form signalling‐competent receptor complexes. Ligand perception by LRR‐RLPs initiates recruitment of the co‐receptor BRI1‐Associated Kinase 1/Somatic Embryogenesis Receptor Kinase 3 (BAK1/SERK3) to the LRR‐RLP/SOBIR1 complex, thereby activating LRR‐RLP‐mediated immunity. We employed phosphorylation analysis of in planta ‐produced proteins, live cell imaging, gene silencing and co‐immunoprecipitation to investigate the roles of SOBIR1 and BAK1 in immune signalling. We show that Arabidopsis thaliana ( At ) SOBIR1, which constitutively activates immune responses when overexpressed in planta , is highly phosphorylated . Moreover, in addition to the kinase activity of SOBIR1 itself, kinase‐active BAK1 is essential for At SOBIR1‐induced constitutive immunity and for the phosphorylation of At SOBIR1. Furthermore, the defence response triggered by the tomato LRR‐RLP Cf‐4 on perception of Avr4 from the extracellular pathogenic fungus Cladosporium fulvum is dependent on kinase‐active BAK1. We argue that, in addition to the trans‐autophosphorylation of SOBIR1, it is likely that SOBIR1 and BAK1 transphosphorylate, and thereby activate the receptor complex. The signalling‐competent cell surface receptor complex subsequently activates downstream cytoplasmic signalling partners to initiate RLP‐mediated immunity.

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“…, ; Wang et al. , ). Here, we show that the kinase activity of At SOBIR1 is essential for its phosphorylation.…”

Section: Discussionmentioning

confidence: 99%

“…, ; Wang et al. , ). Pre‐formation of immune complexes at nanoclusters at the PM, without their activation by the matching ligand, has been shown previously (Bücherl et al.…”

Section: Discussionmentioning

confidence: 99%

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Kinase activity of SOBIR1 and BAK1 is required for immune signalling

Burgh

Postma

Robatzek

et al. 2019

Molecular Plant Pathology

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“…Some apoplastic or cytoplasmic effectors from Phytophthora pathogens display elicitor activity, that is, they could trigger plant immunity responses, and concurrently contribute to the virulence or promote pathogen colonization. Examples include PsXEG1 (Ma et al , , ; Wang et al , ), Avh238 (Yang et al , ), and PpE4 (Huang et al , ). Another similar example was reported in the cell death‐inducing RXLR effector, PcAvh1, which is a virulence factor of P. capsici as its deletion mutants displayed reduced pathogenicity in contrast to the more aggressive overexpression mutants (Chen et al , ).…”

Section: Discussionmentioning

confidence: 99%

An RXLR effector PlAvh142 from Peronophythora litchii triggers plant cell death and contributes to virulence

Situ

Jiang

Fan

et al. 2020

Molecular Plant Pathology

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Litchi downy blight, caused by the phytopathogenic oomycete Peronophythora litchii, results in tremendous economic loss in litchi production every year. To successfully colonize the host cell, Phytophthora species secret hundreds of RXLR effectors that interfere with plant immunity and facilitate the infection process. Previous work has already predicted 245 candidate RXLR effector‐encoding genes in P. litchii, 212 of which have been cloned and tested for plant cell death‐inducing activity in this study. We found three such RXLR effectors could trigger plant cell death through transient expression in Nicotiana benthamiana. Further experiments demonstrated that PlAvh142 could induce cell death and immune responses in several plants. We also found that PlAvh142 localized in both the cytoplasm and nucleus of plant cells. The cytoplasmic localization was critical for its cell death‐inducing activity. Moreover, deletion either of the two internal repeats in PlAvh142 abolished the cell death‐inducing activity. Virus‐induced gene silencing assays showed that cell death triggered by PlAvh142 was dependent on the plant transduction components RAR1 (require for Mla12 resistance), SGT1 (suppressor of the G2 allele of skp1) and HSP90 (heat shock protein 90). Finally, knockout of PlAvh142 resulted in significantly attenuated P. litchii virulence on litchi plants, whereas the PlAvh142‐overexpressed mutants were more aggressive. These data indicated that PlAvh142 could be recognized in plant cytoplasm and is an important virulence RXLR effector of P. litchii.

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“…More recently, a proteinaceous PAMP that induces cell death in Solanaceae plants was identified in ascomycete fungi (Franco-Orozco et al, 2017). Moreover, there are also PAMPs that are widely spread across microbial taxa, such as the necrosis and ethylene-inducing peptide 1-like proteins (NLPs), and the glycoside hydrolase 12 protein XEG1 (Qutob et al, 2006;Oome et al, 2014;Albert et al, 2015;Ma et al, 2015;Wang et al, 2018). Both NLPs and XEG1 are extensively distributed in oomycetes, fungi, and bacteria.…”

Section: Introductionmentioning

confidence: 99%

A small cysteine‐rich protein from two kingdoms of microbes is recognized as a novel pathogen‐associated molecular pattern

et al. 2019

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Summary Pathogen‐associated molecular patterns (PAMPs) are conserved molecules that are crucial for normal life cycle of microorganisms. However, the diversity of microbial PAMPs is little known. During screening of cell‐death‐inducing factors from the necrotrophic fungus Valsa mali, we identified a novel PAMP VmE02 that is widely spread in oomycetes and fungi. Agrobacterium tumefaciens‐mediated transient expression or infiltration of recombinant protein produced by Escherichia coli was performed to assay elicitor activity of the proteins tested. Virus‐induced gene silencing in Nicotiana benthamiana was used to determine the components involved in VmE02‐triggered cell death. The role of VmE02 in virulence and conidiation of V. mali were characterized by gene deletion and complementation. We found that VmE02, together with some of its homologues from both oomycete and fungal species, exhibited cell‐death‐inducing activity in N. benthamiana. VmE02‐triggered cell death was shown to be dependent on BRI1‐ASSOCIATED KINASE‐1, SUPPRESSOR OF BIR1‐1, HSP90 and SGT1 in N. benthamiana. Deletion of VmE02 in V. mali greatly attenuated pathogen conidiation but not virulence, and treatment of N. benthamiana with VmE02 enhances plant resistance to Sclerotinia sclerotiorum and Phytophthora capsici. We conclude that VmE02 is a novel cross‐kingdom PAMP produced by several fungi and oomycetes.

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Leucine-rich repeat receptor-like gene screen reveals that Nicotiana RXEG1 regulates glycoside hydrolase 12 MAMP detection

Cited by 150 publications

References 66 publications

Kinase activity of SOBIR1 and BAK1 is required for immune signalling

Kinase activity of SOBIR1 and BAK1 is required for immune signalling

An RXLR effector PlAvh142 from Peronophythora litchii triggers plant cell death and contributes to virulence

A small cysteine‐rich protein from two kingdoms of microbes is recognized as a novel pathogen‐associated molecular pattern

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