Leucine depletion extends the lifespans of leucine-auxotrophic fission yeast by inducing Ecl1 family genes via the transcription factor Fil1

Ohtsuka, Hokuto; Kato, Takanori; Sato, Tomohiro; Shimasaki, Takafumi; Kojima, Takaaki; Aiba, Hirofumi

doi:10.1007/s00438-019-01592-6

Cited by 20 publications

(53 citation statements)

References 41 publications

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“…Real‐time PCR analysis was performed as described previously (Ohtsuka et al., 2019). The oligonucleotides used in this experiment are listed in Table S2.…”

Section: Methodsmentioning

confidence: 99%

Sulfur depletion induces autophagy through Ecl1 family genes in fission yeast

et al. 2020

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Autophagy comprises one of the intracellular degradation systems conserved from yeasts to humans. This system plays a vital role in the degradation of intracellular components such as proteins and organelles in the processes of their exclusion and turnover. Autophagy is also necessary for adaptation to environmental changes, including depletion of various nutrients. Till date, several autophagy-related genes and mechanisms have been identified, wherein the budding yeast Saccharomyces cerevisiae has significantly contributed to this identification (Mizushima et al., 2011; Ohsumi, 2014; Xie & Klionsky, 2007). Although autophagy is widely conserved, there exist some differences in the conditions that induce autophagy and its related factors among species. For instance, autophagy is induced by carbon depletion in S. cerevisiae, but not in Schizosaccharomyces pombe (Mukaiyama et al., 2009). Moreover, autophagy factors are partially different between these two species (Mukaiyama et al., 2010). Yeasts have also contributed to lifespan studies, and a chronological lifespan is defined as the survival period after entry into the stationary phase (Fabrizio & Longo, 2003). Earlier, the Ecl1 family genes (ecl1 + , ecl2 + and ecl3 + , respectively) were identified whose over-expression was found to cause the extension of the chronological lifespan in S. pombe (Ohtsuka et al., 2008, 2009). These genes encode small proteins consist of about 80 amino acids, respectively, but their molecular functions have been unclear. However, phenotypic analysis revealed that Ecl1 family genes are also essential for the appropriate cellular response to sulfur depletion (Ohtsuka et al., 2017). Sulfur is one of the essential nutrients and is present in sulfur compounds such as biotin, thiamine, coenzyme A, lipoic acid and sulfur amino acid. Sulfur depletion induces cellular responses such as lifespan extension, morphological changes, and cell cycle arrest in S. pombe, and these phenomena are almost completely dependent on the Ecl1 family genes (Ohtsuka et al., 2017).

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“…Real‐time PCR analysis was performed as described previously (Ohtsuka et al., 2019). The oligonucleotides used in this experiment are listed in Table S2.…”

Section: Methodsmentioning

confidence: 99%

Sulfur depletion induces autophagy through Ecl1 family genes in fission yeast

et al. 2020

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“…The induction of ecl1 + transcription also occurs by arginine (Arg), lysine (Lys), and histidine (His) depletion in the corresponding auxotrophs (Ohtsuka et al, 2019). Therefore, we investigated ecl1 + expression in Arg, Lys, and His auxotrophs starved of Mg 2+ (Figure 1c).…”

Section: Mg 2+ Depletion Induces Ecl1 + Expressionmentioning

confidence: 99%

“…The induction of ecl1 + by sulfur depletion can be observed in both prototrophic and auxotrophic strains; however, this induction can also be observed in amino acid auxotrophic strains due to amino acid depletion (Ohtsuka et al, 2019). The latter is mediated by the GATA‐type transcription factor Fil1 (Ohtsuka et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Activated Gcn2 phosphorylates eIF2α, decreasing global translation initiation efficiency, concomitant with an increased translation of GCN4 mRNA, encoding a transcriptional activator of numerous genes involved in amino acid metabolism, and the salvaging of nutrients (González & Hall, 2017; Tarumoto et al, 2013). We showed that Ecl1 family genes, one of which is induced by Fil1, are essential for survival when cells are starved of leucine (Leu; Ohtsuka et al, 2019). Despite evidence that establishes the relationship between the evolutionarily conserved GAAC/AAR pathway and Ecl1 family genes, knowledge of the GAAC pathway of fission yeast is limited.…”

Section: Introductionmentioning

confidence: 99%

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Magnesium depletion extends fission yeast lifespan via general amino acid control activation

et al. 2021

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“…However, unlike S. pombe, which has three Ecl1 family genes, S. cerevisiae has only one, that is, ECL1, the overexpression of which extends CLS and deletion shortens CLS (Azuma et al, 2009). Recently, Ecl1 family genes were found to be involved in CLS regulation as downstream factors of the general amino acid control (amino acid response in mammals) (Ohtsuka et al, 2019). Nevertheless, the orthologs of this gene have not been found in higher organisms.…”

Section: Ecl1 Family G Ene Smentioning

confidence: 99%

Genes affecting the extension of chronological lifespan in Schizosaccharomyces pombe (fission yeast)

Ohtsuka

Shimasaki

Aiba

2020

Molecular Microbiology

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The fission yeast Schizosaccharomyces pombe is a model organism of unicellular eukaryotes (Hayles and Nurse, 2018). This yeast is considered to have diverged from the budding yeast Saccharomyces cerevisiae hundreds of million years ago (Hayles and Nurse, 2018;Hedges, 2002;Sipiczki, 2000), and studies using these yeast species have contributed significantly to the understanding of various cellular processes. S. pombe has been actively used for research in multiple fields, including cell cycle studies, cellular morphology, sexual development, splicing, and chromosome structure. Furthermore, much information is accumulating regarding the stationary phase of cells,

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Leucine depletion extends the lifespans of leucine-auxotrophic fission yeast by inducing Ecl1 family genes via the transcription factor Fil1

Cited by 20 publications

References 41 publications

Sulfur depletion induces autophagy through Ecl1 family genes in fission yeast

Sulfur depletion induces autophagy through Ecl1 family genes in fission yeast

Magnesium depletion extends fission yeast lifespan via general amino acid control activation

Genes affecting the extension of chronological lifespan in Schizosaccharomyces pombe (fission yeast)

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