Leucaena leucocephala serine proteinase inhibitor: primary structure and action on blood coagulation, kinin release and rat paw edema

Oliva, Maria Luíza Vilela; Souza-Pinto, Jose Carlos; Batista, Isabel de Fátima Correia; Araújo, Magali; Silveira, Vera F; Auerswald, Ennes A.; Mentele, Reini; Eckerskorn, Christoph; Sampaio, Misako U.; Sampaio, Cláudio Hoffmann

doi:10.1016/s0167-4838(99)00285-x

Cited by 117 publications

(79 citation statements)

References 29 publications

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“…as for other inhibitors described from the Mimosoideae (Batista et al, 1996;Oliva et al, 2000;Vargas et al, 2004). In agreement with these data, mass spectrometry analyses were measured from the SEC samples, as they indicated the presence of three isoforms with the following molecular mass : 19,709 Da,19,869 and 20,378 Da (data not shown).…”

Section: Structural Characterizationmentioning

confidence: 82%

“…2A). The sequential alignment of ApTI isoinhibitors established identity with Kunitz type inhibitors from Acacia (Kortt and Jermyn, 1981;Lin et al, 1991) and other Leguminosae (Oliva et al, 2000;Negreiros et al, 1991;Odani et al, 1979).…”

Section: Structural Characterizationmentioning

confidence: 97%

“…The fluorescence experiments are also in agreement with these data, as there was no alteration in the k max of the emission in the whole pH range (even at extreme alkaline or acid values). Fluorescence quenching (Lin et al, 1991), AeTI: Acacia elata trypsin inhibitor (Kortt and Jermyn, 1981), PjTI: Prosopsis juliflora trypsin inhibitor (Negreiros et al, 1991), LlTI: Leucaena leucocephala trypsin inhibitor (Oliva et al, 2000), STI: soybean trypsin inhibitor (Kunitz, 1945), silktree: silktree trypsin inhibitor (Odani et al, 1979). The conserved amino acids residues are highlighted in bold.…”

Section: Structural Characterizationmentioning

confidence: 99%

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Physico-chemical and antifungal properties of protease inhibitors from Acacia plumosa

et al. 2009

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Section: Structural Characterizationmentioning

confidence: 82%

Section: Structural Characterizationmentioning

confidence: 97%

Section: Structural Characterizationmentioning

confidence: 99%

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Physico-chemical and antifungal properties of protease inhibitors from Acacia plumosa

et al. 2009

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“…The cuticle of H. contortus eggs has three layers: an internal layer composed of lipids with some associated proteins; an intermediate layer composed of chitin fibers surrounded by proteins; and an outer layer composed of proteins and lipids (MANSFIELD et al, 1992). Different classes of proteases are known to be inactivated by plant proteins, called protease inhibitors (OLIVA et al, 2000). Gastrointestinal nematodes produce a variety of proteases that have different functions during development (RHOADS et al, 2000;WILLIAMSON et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic activity of Leucaena leucocephala protein extracts on Haemonchus contortus

Soares

Araújo

Lopes

et al. 2015

Rev. Bras. Parasitol. Vet.

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The objective of this study was to evaluate the effects of protein extracts obtained from the plant Leucaena leucocephala on the nematode parasite Haemonchus contortus. The seeds, shell and cotyledon of L. leucocephala were separated and their proteins extracted using a sodium phosphate buffer, and named as TE (total seed extract), SE (shell extract) and CE (cotyledon extract). Soluble protein content, protease, protease inhibitory and chitinase activity assays were performed. Exsheathment inhibition of H. contortus larvae were performed at concentrations of 0.6 mg mL . The effective concentration for 50% hatching inhibition (EC 50 ) was estimated by probit. Different proportions of soluble proteins, protease and chitinase were found in TE and CE. Protease inhibitory activity was detected in all extracts. The EC 50 of the CE and TE extracts were 0.48 and 0.33 mg mL -1 , respectively. No ovicidal effects on H. contortus were detected in SE extracts, and none of the protein extracts demonstrated larvicidal effects on H. contortus. We therefore conclude that protein extracts of L. leucocephala had a detrimental effect on nematode eggs, which can be correlated with the high protease and chitinase activity of these extracts.Keywords: Plant proteins, protease, protease inhibitor, chitinase. ResumoO objetivo deste estudo foi avaliar a atividade anti-helmíntica de extratos proteicos de leucena (Leucaena leucocephala) sobre Haemonchus contortus. As sementes, as cascas e os cotilédones foram moídos separadamente e as proteínas extraídas com tampão fosfato de sódio e denominados: TE (extrato total), SE (extrato casca) e CE (extrato cotilédone). O teor de proteínas, atividade proteolítica, inibitória de protease e quitinolítica dos extratos foram verificados, além da ação sobre a eclosão de ovos e desembainhamento larvar de H. contortus. A concentração efetiva para inibição de 50% da eclosão dos ovos (EC 50 ) foi calculada através do probit. Foi demonstrado que TE e CE possuem, em diferentes proporções, proteínas solúveis, protease e quitinase. Atividade inibitória de protease foi encontrada em todos os extratos. A EC 50 dos extratos CE e TE foram 0,48 e 0,33 mg de proteína mL -1 , respectivamente. O extrato SE não apresentou atividade sobre a eclosão dos ovos. Os extratos proteicos não apresentaram efeito larvicida sobre H. contortus. Conclui-se que a ação de extratos proteicos de L. leucocephala afetam negativamente a eclodibilidade dos ovos, correlacionando-se com a alta atividade de protease e quitinase dos extratos testados.Palavras-chaves: Proteínas vegetais, protease, inibidor de protease, quitinase.

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“…However, interest in characterizing new PIs and understanding their physiological significance has increased due to their biological relevance for all living processes, such as blood coagulation system, complement cascade, apoptosis, cell cycle and hormone processing pathways [17,[23][24][25]. Furthermore, deficiencies or alterations in the regulation of these enzymes lead several pathological conditions, such as cancer, arthritis, neurodegenerative and cardiovascular diseases [26,27].…”

Section: Introductionmentioning

confidence: 99%

Discoveries of Serine Protease Inhibitors from Scorpions

Hakim¹,

Yang²

2016

J Proteomics Bioinform

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Leucaena leucocephala serine proteinase inhibitor: primary structure and action on blood coagulation, kinin release and rat paw edema

Cited by 117 publications

References 29 publications

Physico-chemical and antifungal properties of protease inhibitors from Acacia plumosa

Physico-chemical and antifungal properties of protease inhibitors from Acacia plumosa

Anthelmintic activity of Leucaena leucocephala protein extracts on Haemonchus contortus

Discoveries of Serine Protease Inhibitors from Scorpions

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