Lesion development factors following inoculation ofSclerotinia sclerotiorum mycelium to eggplant, tomato and French bean

Torés, J. A.; Moreno, R.

doi:10.1007/bf02979548

Cited by 5 publications

(4 citation statements)

References 7 publications

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“…Genotype × isolate interaction for LL was not significant (P > 0.05) (Table 3). Nelson et al (1991), using a similar assay with soybean, did not observe a significant genotype × isolate interaction, nor have other researchers in similar studies with S. sclerotiorum (Morrall et al, 1971;Tores and Moreno, An isolate × repetition interaction (P < 0.05) for LL (Table 3) occurred because isolate ND16 was less virulent in Repetition 2 than in 1. Slight changes in environment and heterogeneity for virulence among sclerotia of the isolate may have contributed to the reduced virulence of ND16 in Repetition 2.…”

Section: -Day Assay Of Bean Genotype Resistancementioning

confidence: 71%

“…Researchers have used other means to measure physiological resistance of stem tissue to S. sclerotiorium without bias. Tores and Moreno (1987) used lesion perimeter or stem diameter to calculate infected stem volume, and Sedun et al (1989) used a lesion expansion rate to estimate PPR of stem tissue. We tried to estimate infected stem volume with stem diameter and displacement of water in graduated cylinders, but measurement was too difficult.…”

Section: Stem Size and Lesion Characteristicsmentioning

confidence: 99%

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Screening for Partial Physiological Resistance to White Mold in Dry Bean Using Excised Stems

Miklas¹,

Grafton²,

Nelson³

1992

jashs

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A laboratory procedure was tested to determine whether excised stems would allow a reliable indication of partial physiological resistance (PPR) to white mold [Sclerotinia sclerotiorum (Lib.) deBary] in dry bean (Phaseolus vulgaris L.). Excised stems from 11- and 28-day-old plants were inoculated with growing mycelium of S. sclerotiorum, incubated for 4 to 7 days (11- and 28-day assays, respectively), then assayed for lesion length (LL). A total of 15 bean genotypes were screened for PPR, as indicated by LL. Significant (P < 0.05) differences among LL means of small- and medium-seeded bean genotypes were detected in the 28-day assay, whereas only LL means among medium-seeded genotypes. differed significantly (P < 0.05) in the n-day assay. `Bunsi', `C-20', `Sierra', `Topaz', and snap bean breeding lines NY 5262, NY 5394, and NY 5403 had the highest PPR and `Upland', D76125, and `UI-114' the lowest. The results from both assays were repeatable. A moderately high correlation (r = 0.68, P < 0.02) was observed between PPR and field resistance. The 28-day assay has potential for evaluating dry bean germplasm for PPR to white mold disease caused by S. sclerotiorum. A 28-day assay also was used to measure virulence of 18 isolates of S. sclerotiorum. The 18 isolates did not differ (P < 0.05) for virulence when measured by LL. The lack of any genotype × isolate interaction for LL indicated lack of host-pathogen specificity.

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Section: -Day Assay Of Bean Genotype Resistancementioning

confidence: 71%

Section: Stem Size and Lesion Characteristicsmentioning

confidence: 99%

Screening for Partial Physiological Resistance to White Mold in Dry Bean Using Excised Stems

Miklas¹,

Grafton²,

Nelson³

1992

jashs

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“…de Bary is a ubiquitous, destructive plant pathogen with a broad spectrum of over 408 host plant species, including important economic crops and weeds. [1][2][3] Multiple vegetative components, such as root, stem, leaf and fruit, can be severely affected by this pathogen at different plant growth stages, causing large loss of yield and quality in many countries. [4][5][6][7] Although efforts have been made to breed resistant cultivars, the disease is still a major threat to crop production because of limited gene resources for disease resistance.…”

Section: Introductionmentioning

confidence: 99%

“…Sclerotinia sclerotiorum (Lib.) de Bary is a ubiquitous, destructive plant pathogen with a broad spectrum of over 408 host plant species, including important economic crops and weeds . Multiple vegetative components, such as root, stem, leaf and fruit, can be severely affected by this pathogen at different plant growth stages, causing large loss of yield and quality in many countries .…”

Section: Introductionmentioning

confidence: 99%

The relationship between features enabling SDHI fungicide binding to the Sc‐Sdh complex and its inhibitory activity against Sclerotinia sclerotiorum

Gao

Zhu

et al. 2020

Pest Management Science

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BACKGROUNDA new generation of succinate dehydrogenase inhibitors (SDHIs) with high efficiency and broad‐spectrum antifungal activity has been frequently used in crop production. Sclerotinia stem rot is a major disease of various plants and crops caused by Sclerotinia sclerotiorum. Although benzovindiflupyr and isopyrazam reportedly have high activity against S. sclerotiorum, little is known about the bioactivity of different SDHIs classes against S. sclerotiorum or the mechanism of their differential antifungal activity.RESULTSThe in vitro tests revealed that the pyrazole‐4‐carboxamides of SDHIs (benzovindiflupyr, isopyrazam, fluxapyroxad, pydiflumetofen) had the highest activity against S. sclerotiorum followed by pyridine carboxamides (boscalid), pyridinyl‐ethyl benzamides (fluopyram) and thiazole carboxamides (thifluzamide), and of these thifluzamide showed poor antifungal activity with EC50 values greater than 6.01 mg L−1. The pyrazole‐4‐carboxamides of SDHIs showed satisfactory protective and curative activity against Sclerotinia stem rot. After treatment with the pyrazole‐4‐carboxamides of SDHIs, mitochondrial function in S. sclerotiorum decreased significantly. The enzyme activity assays revealed a lower affinity between thifluzamide and the Sc‐Sdh complex than was observed for the other six fungicides, with IC50 values ranging from 0.0036 to 1.2088 μmol L−1. Additionally, the docking positions of fungicides were similar, yet binding energies were different in the docking study with the Sdh complex. The correspondingly weaker hydrogen bonds may be responsible for the poor activity of thifluzamide against S. sclerotiorum.CONCLUSIONUnderstanding different binding features of various SDHIs classes with the Sc‐Sdh complex might be beneficial for the design and development of highly effective broad‐spectrum fungicides to ensure high yield and quality in crops by reducing fungicide use. © 2020 Society of Chemical Industry

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Favorable Bioactivity of the SDHI Fungicide Benzovindiflupyr Against Sclerotinia sclerotiorum Mycelial Growth, Sclerotial Production, and Myceliogenic and Carpogenic Germination of Sclerotia

et al. 2019

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Sclerotinia sclerotiorum, which can cause Sclerotinia stem rot, is a prevalent plant pathogen. This study aims to evaluate the application potential of benzovindiflupyr, a new generation of succinate dehydrogenase inhibitor (SDHI), against S. sclerotiorum. In our study, 181 isolates collected from different crops (including eggplant [n = 34], cucumber [n = 27], tomato [n = 29], pepper [n = 35], pumpkin [n = 32], and kidney bean [n = 25]) in China were used to establish baseline sensitivity to benzovindiflupyr. The frequency distribution of the 50% effective concentration (EC50) values of benzovindiflupyr was a unimodal curve, with mean EC50 values of 0.0260 ± 0.011 μg/ml, and no significant differences in mean EC50 existed among the various crops (P > 0.99). Benzovindiflupyr can effectively inhibit mycelial growth, sclerotial production, sclerotial shape, and myceliogenic and carpogenic germination of the sclerotia of S. sclerotiorum. In addition, benzovindiflupyr showed good systemic translocation in eggplant. Using benzovindiflupyr at 100 μg/ml yielded efficacies of 71.3 and 80.5% for transverse activity and cross-layer activity, respectively, which were higher than those of acropetal and basipetal treatments (43.6 and 44.7%, respectively). Greenhouse experiments were then carried out at two experimental sites for verification. Applying benzovindiflupyr at 200 g a.i. ha−1 significantly reduced the disease incidence and severity of Sclerotinia stem rot. Overall, the results demonstrated that benzovindiflupyr is a potential alternative product to control Sclerotinia stem rot.

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Lesion development factors following inoculation ofSclerotinia sclerotiorum mycelium to eggplant, tomato and French bean

Cited by 5 publications

References 7 publications

Screening for Partial Physiological Resistance to White Mold in Dry Bean Using Excised Stems

Screening for Partial Physiological Resistance to White Mold in Dry Bean Using Excised Stems

The relationship between features enabling SDHI fungicide binding to the Sc‐Sdh complex and its inhibitory activity against Sclerotinia sclerotiorum

Favorable Bioactivity of the SDHI Fungicide Benzovindiflupyr Against Sclerotinia sclerotiorum Mycelial Growth, Sclerotial Production, and Myceliogenic and Carpogenic Germination of Sclerotia

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