Les phlébotomes (Diptera : Psychodidae) de la Guyane Française. I - Description du mâle de<i>Lutzomyia (Trichopygomyia) depaquiti</i>n. sp.

Gantier, Jean-Charles; Gaborit, Pascal; Rabarison, Patrick

doi:10.1051/parasite/2006131011

Cited by 3 publications

(5 citation statements)

References 5 publications

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“…After implementation of mass spectra from the validation panel, the MALDI-TOF MS reference database comprises 29 species (282 mass spectra), accounting for about 36% of the 81 sand fly species previously described in French Guiana and may cover the most common species [38–40], including those dominating the communities in a large diversity of forested habitats, encountering different environmental stress levels.…”

Section: Discussionmentioning

confidence: 99%

Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library

et al. 2019

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Phlebotomine sand flies are insects that are highly relevant in medicine, particularly as the sole proven vectors of leishmaniasis. Accurate identification of sand fly species is an essential prerequisite for eco-epidemiological studies aiming to better understand the disease. Traditional morphological identification is painstaking and time-consuming, and molecular methods for extensive screening remain expensive. Recent studies have shown that matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a promising tool for rapid and cost-effective identification of arthropod vectors, including sand flies. The aim of this study was to validate the use of MALDI-TOF MS for the identification of Northern Amazonian sand flies. We constituted a MALDI-TOF MS reference database comprising 29 species of sand flies that were field-collected in French Guiana, which are expected to cover many of the more common species of the Northern Amazonian region, including known vectors of leishmaniasis. Carrying out a blind test, all the sand flies tested ( n = 157) with a log (score) threshold greater than 1.7 were correctly identified at the species level. We confirmed that MALDI-TOF MS protein profiling is a useful tool for the study of sand flies, including neotropical species, known for their great diversity. An application that includes the spectra generated here will be available to the scientific community in the near future via an online platform.

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Section: Discussionmentioning

confidence: 99%

Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library

et al. 2019

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“…; Young & Duncan ; Gantier et al . ; a complete checklist of French Guiana species is presented in the Supporting information). Sequences were obtained for 178 morphologically identified specimens representing 38 species of the genus Lutzomyia and two species of the genus Brumptomyia .…”

Section: Resultsmentioning

confidence: 99%

“…Species identifications were performed using the taxonomic guide of Young & Duncan (), which includes all the references for the descriptions of sand fly species found in French Guiana and Trinidad and Tobago, except for L. depaquiti described later (Gantier et al . ). Specimens were then stored as vouchers.…”

Section: Methodsmentioning

confidence: 97%

“…The morphological and morphometrical data were obtained with the DM5500B LeicaTM microscope associated with the calibration software LEICA APPLI-CATION SUITE 3.8.0. Species identifications were performed using the taxonomic guide of Young & Duncan (1994), which includes all the references for the descriptions of sand fly species found in French Guiana and Trinidad and Tobago, except for L. depaquiti described later (Gantier et al 2006). Specimens were then stored as vouchers.…”

Section: Reference Library Of Dna Metabarcodesmentioning

confidence: 99%

“…About 80 species of sand flies have been found in French Guiana, most of them belonging to the genus Lutzomyia (L eger et al 1977;Young & Duncan 1994;Gantier et al 2006; a complete checklist of French Guiana species is presented in the Supporting information). Sequences were obtained for 178 morphologically identified specimens representing 38 species of the genus Lutzomyia and two species of the genus Brumptomyia.…”

Section: Reference Library Of Dna Metabarcodesmentioning

confidence: 99%

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Vector soup: high‐throughput identification of Neotropical phlebotomine sand flies using metabarcoding

Kocher

Gantier²,

Gaborit

et al. 2016

Molecular Ecology Resources

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Phlebotomine sand flies are haematophagous dipterans of primary medical importance. They represent the only proven vectors of leishmaniasis worldwide and are involved in the transmission of various other pathogens. Studying the ecology of sand flies is crucial to understand the epidemiology of leishmaniasis and further control this disease. A major limitation in this regard is that traditional morphological-based methods for sand fly species identifications are time-consuming and require taxonomic expertise. DNA metabarcoding holds great promise in overcoming this issue by allowing the identification of multiple species from a single bulk sample. Here, we assessed the reliability of a short insect metabarcode located in the mitochondrial 16S rRNA for the identification of Neotropical sand flies, and constructed a reference database for 40 species found in French Guiana. Then, we conducted a metabarcoding experiment on sand flies mixtures of known content and showed that the method allows an accurate identification of specimens in pools. Finally, we applied metabarcoding to field samples caught in a 1-ha forest plot in French Guiana. Besides providing reliable molecular data for species-level assignations of phlebotomine sand flies, our study proves the efficiency of metabarcoding based on the mitochondrial 16S rRNA for studying sand fly diversity from bulk samples. The application of this high-throughput identification procedure to field samples can provide great opportunities for vector monitoring and eco-epidemiological studies.

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Overview of Mitaraka survey: research frame, study site and field protocols

2018

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Zoosystema est indexé dans / Zoosystema is indexed in:-Science Citation Index Expanded (SciSearch ® ) -ISI Alerting Services ® -Current Contents ® / Agriculture, Biology, and Environmental Sciences ® -Scopus ® Zoosystema est distribué en version électronique par / Zoosystema is distributed electronically by: -BioOne ® (http://www.bioone.org) Les articles ainsi que les nouveautés nomenclaturales publiés dans Zoosystema sont référencés par / Articles and nomenclatural novelties published in Zoosystema are referenced by: -ZooBank ® (http://zoobank.org) PHOTOCOPIES : Les Publications scientifiques du Muséum adhèrent au Centre Français d'Exploitation du Droit de Copie (CFC), 20 rue des Grands Augustins, 75006

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Les phlébotomes (Diptera : Psychodidae) de la Guyane Française. I - Description du mâle deLutzomyia (Trichopygomyia) depaquitin. sp.

Cited by 3 publications

References 5 publications

Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library

Identification of French Guiana sand flies using MALDI-TOF mass spectrometry with a new mass spectra library

Vector soup: high‐throughput identification of Neotropical phlebotomine sand flies using metabarcoding

Overview of Mitaraka survey: research frame, study site and field protocols

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