Leptospirosis in Caspian Sea littoral, Gilan Province, Iran

Garshasbi, Vahid; Naddaf, Saied Reza; Aghighi, Zohreh; Hassan, Nayerreh; Pooya, Mohammad; Mostafavi, Ehsan

doi:10.1016/j.actatropica.2018.01.010

Cited by 6 publications

(7 citation statements)

References 21 publications

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“…Future studies with larger sample sizes may provide enough statistical power to detect real associations better. Our finding is in agreement with previous similar studies in the Caspian Sea littoral [9,16], which showed that by the removal or reduction of Leptospira spirochete in blood by the patient's immune system, the antibodies titers increase. Previously, we showed that during the infection, the IFA becomes positive much later than PCR [16].…”

Section: Discussionsupporting

confidence: 94%

“…The IFA exhibited a reliable specificity and sensitivity compared with the MAT for the initial diagnosis of leptospirosis [21][22]. Our housemade IFA was also shown to be a reliable tool for diagnosis and epidemiological surveys of leptospirosis [9,16,17].…”

Section: Discussionmentioning

confidence: 99%

“…IFA. The serum samples were screened by a housemade IFA, as described previously [9,16,17]. The positive controls were the pooled sera from leptospirosis patients in the Caspian Sea littoral referred to health care centers during transmission seasons and had anti-Leptospira antibodies titers ≥1/160 by IFA.…”

Section: Methodsmentioning

confidence: 99%

“…Nested PCR. A 291 bp sequence was amplified by a nested PCR assay using the primers complementary to the rrs sequence (Table 1), and the reagents and protocols successfully practiced by others [6,16]. Previously, we could detect as low as ≥ 0.6 spirochete genome per reaction by this method [16].…”

Section: Methodsmentioning

confidence: 99%

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Diagnosis of Leptospirosis in Febrile Patients of Golestan Province, Iran: Serology versus PCR

Naddaf

Mohabati

Vahabpor

et al. 2020

JoMMID

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Introduction: Leptospirosis is a significant public health problem in the Caspian littoral of Iran comprising Gilan, Mazandaran, and Golestan provinces. In Golestan province, serology assays indicated anti-Leptospira antibodies in animals and humans; however, no reliable record of infections in patients with signs and symptoms of the disease is available. Methods: We employed the indirect immunofluorescent antibody assay (IFA), and two PCR assays, a realtime PCR (qPCR) and a nested-PCR targeting 16S rDNA (rrs) sequence for diagnosis of leptospirosis in febrile patients in Golestan province. Results: Out of 52 febrile patients, 25 (48.07%) had antibody titers ≥1/80 by IFA, and were defined as positive. In 7, 9, and 7 individuals, the antibody titers were 1/40, 1/20, and 1/10, respectively, and 4 had no antibodies. The qPCR and nested PCR detected leptospiral DNA in 55.75% and 67.3% of the patients' sera, respectively. The two PCR assays had a Kappa agreement of 0.53 (P< 0.0001), suggesting a moderate agreement, and showed a significant reverse association with the IFA titers (P<0.05). Conclusion: Our results suggest that higher antibody titers accompanied the spirochete's removal from blood by the patient's immune response. Hence, a reliable diagnosis of Leptospira infection necessitates deploying a DNA-based method alongside serology.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Diagnosis of Leptospirosis in Febrile Patients of Golestan Province, Iran: Serology versus PCR

Naddaf

Mohabati

Vahabpor

et al. 2020

JoMMID

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“…Thus, most people with the disease have been involved in agriculture as their mainoccupation. [21][22][23] In many surveys, 17,18,24,25 including our study, leptospirosis was most common in middleaged people (40-60 years). In our patients, the mean hospitalization days were lower than in the study by Goris et al, 26 which could be due to the fact that in the study by Goris et al, 26 people with severe forms of the disease were examined.…”

Section: Discussionmentioning

confidence: 94%

Relationship Between the Prevalence of Blood Groups and Severity of Leptospirosis: A Case-Control Study

et al. 2020

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Introduction: The correlation between the prevalence and severity of leptospirosis with blood groups has not been investigated so far, but several studies have been conducted to link the infectious diseases with blood groups. The aim of this study was to investigate the prevalence of blood type in patients with leptospirosis and its association with disease severity. Methods: This is a case-control study performed on hospitalized patients with the diagnosis of leptospirosis in Mazandaran province, Iran, in 2018. The control group was selected from among the families of patients. Blood groups (ABO and Rh) and severity of the disease were assessed. Data were analyzed by SPSS 22. Results: A total of 300 people (150 in the case and 150 in the control) enrolled in the study. The mean age was 44.35 ± 15.39 years and 81.3% were men. The highest frequency of blood type in both groups was O+, A+, and B+, respectively. There was a statistically significant difference in the frequency of blood groups ( P = .037). Comparison between severity of disease and blood types (ABO, Rh) showed no significant difference ( P > .05). Conclusions: According to our study, O+ was the most common among patients with leptospirosis. The frequency of O in patients was significantly higher than in the control group, but there was no significant relationship between leptospirosis and Rh. The prevalence of this blood type was higher in people with a severe form of the disease. Finally, there is no statistically significant difference between the severity of the disease and ABO and Rh.

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Infection of hard ticks in the Caspian Sea littoral of Iran with Lyme borreliosis and relapsing fever borreliae

Naddaf

Mahmoudi

Ghasemi

et al. 2020

Ticks and Tick-borne Diseases

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Leptospirosis in Caspian Sea littoral, Gilan Province, Iran

Cited by 6 publications

References 21 publications

Diagnosis of Leptospirosis in Febrile Patients of Golestan Province, Iran: Serology versus PCR

Diagnosis of Leptospirosis in Febrile Patients of Golestan Province, Iran: Serology versus PCR

Relationship Between the Prevalence of Blood Groups and Severity of Leptospirosis: A Case-Control Study

Infection of hard ticks in the Caspian Sea littoral of Iran with Lyme borreliosis and relapsing fever borreliae

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