Leptin modulates enteric neurotransmission in the rat proximal colon: An in vitro study

Voinot, Florian; Fischer, Caroline; Crenner, F.; Schmidt, Camille; Angel, Fabielle

doi:10.1016/j.regpep.2013.06.010

Cited by 13 publications

(10 citation statements)

References 57 publications

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“…Interestingly, many substances that act centrally to modulate food intake also influence the activity of gastrointestinal smooth muscle[ 11 - 14 ], whose motor phenomena represent a source of peripheral signals involved in the control of feeding behavior thought the gut-brain axis[ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Adiponectin affects the mechanical responses in strips from the mouse gastric fundus

Idrizaj

Garella

Castellini

et al. 2018

WJG

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AIMTo investigate whether the adipocytes derived hormone adiponectin (ADPN) affects the mechanical responses in strips from the mouse gastric fundus.METHODSFor functional experiments, gastric strips from the fundal region were cut in the direction of the longitudinal muscle layer and placed in organ baths containing Krebs-Henseleit solution. Mechanical responses were recorded via force-displacement transducers, which were coupled to a polygraph for continuous recording of isometric tension. Electrical field stimulation (EFS) was applied via two platinum wire rings through which the preparation was threaded. The effects of ADPN were investigated on the neurally-induced contractile and relaxant responses elicited by EFS. The expression of ADPN receptors, Adipo-R1 and Adipo-R2, was also evaluated by touchdown-PCR analysis.RESULTSIn the functional experiments, EFS (4-16 Hz) elicited tetrodotoxin (TTX)-sensitive contractile responses. Addition of ADPN to the bath medium caused a reduction in amplitude of the neurally-induced contractile responses (P < 0.05). The effects of ADPN were no longer observed in the presence of the nitric oxide (NO) synthesis inhibitor L-NG-nitro arginine (L-NNA) (P > 0.05). The direct smooth muscle response to methacholine was not influenced by ADPN (P > 0.05). In carbachol precontracted strips and in the presence of guanethidine, EFS induced relaxant responses. Addition of ADPN to the bath medium, other than causing a slight and progressive decay of the basal tension, increased the amplitude of the neurally-induced relaxant responses (P < 0.05). Touchdown-PCR analysis revealed the expression of both Adipo-R1 and Adipo-R2 in the gastric fundus.CONCLUSIONThe results indicate for the first time that ADPN is able to influence the mechanical responses in strips from the mouse gastric fundus.

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Section: Introductionmentioning

confidence: 99%

Adiponectin affects the mechanical responses in strips from the mouse gastric fundus

Idrizaj

Garella

Castellini

et al. 2018

WJG

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“…The enteric nervous system (ENS) is important in regulating gut motility, secretion, and nutrient absorption. Leptin has been shown to affect the ENS[ 16 , 17 ] and sensory afferent neurons[ 18 ]. Taken together, leptin may be involved in the regulation of both local gastrointestinal functions and the brain-gut axis.…”

Section: Introductionmentioning

confidence: 99%

Increased intestinal mucosal leptin levels in patients with diarrhea-predominant irritable bowel syndrome

Liu

Yao

2018

WJG

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AIMTo measure the leptin levels in patients with diarrhea-predominant irritable bowel syndrome (IBS-D) and analyze the relationship of leptin with clinical features, visceral sensitivity, mast cells, and nerve fibers.METHODSForty-two patients with IBS-D fulfilling the Rome III criteria and 20 age- and sex-matched healthy controls underwent clinical and psychological evaluations using validated questionnaires (including IBS Symptom Severity Scale, IBS-specific Quality of Life, Hamilton Anxiety Scale, and Hamilton Depression Scale), along with colonoscopy, colonic mucosal biopsy, and visceral sensitivity testing. Serum leptin levels were assayed using enzyme-linked immunosorbent assay. Mucosal leptin expression and localization were evaluated using immunohistochemistry and immunofluorescence. Mucosal leptin mRNA levels were quantified using quantitative real-time reverse transcription polymerase chain reaction. Mast cell counts and activation rates were investigated by toluidine blue staining. Correlation analyses between these parameters were performed.RESULTSThere were no statistically significant differences in age, gender, or body mass index between the IBS-D group and the control group. The median IBS Symptom Severity Scale score in the IBS-D group was 225.0 (range, 100-475). IBS-D patients had significantly increased anxiety [IBS-D: median, 6.5; interquartile range (IQR), 3.3; control: median, 2.0; IQR, 2.0; P < 0.001] and depression (IBS-D: median, 7.0; IQR, 3.0; control: median, 3.0; IQR, 2.0; P < 0.001) scores. IBS-D patients had significantly lower first sensation threshold (IBS-D: median, 50.6; IQR, 25.9; control: median, 80.5; IQR, 18.6; P < 0.001), defecation sensation threshold (IBS-D: median, 91.5; IQR, 29.3; control: median, 155.0; IQR, 21.1; P < 0.001) and maximum tolerable threshold (IBS-D: median, 163.2; IQR, 71.2; control: median, 226.2; IQR, 39.3; P < 0.001). Mucosal leptin expression, as reflected by integrated optical density (IBS-D: median, 4424.71; IQR, 4533.63; control: median, 933.65; IQR, 888.10; P < 0.001), leptin mRNA expression (IBS-D: median, 1.1226; IQR, 1.6351; control: median, 0.8947; IQR, 0.4595; P = 0.009), and mast cell activation rate (IBS-D: median, 71.2%; IQR, 12.9%; control group: median, 59.4%; IQR, 18.88%; P < 0.001) were significantly increased in IBS-D patients. The colocalization of leptin and leptin receptors was observed on mast cells and PGP9.5-positive nerve fibers in the intestinal mucosa. Also, leptin expression was positively correlated with anxiety, depression, and the mast cell activation rate, but negatively correlated with the defecation sensation threshold and the maximum tolerance threshold during visceral sensitivity testing (adjusted P < 0.0038).CONCLUSIONIncreased levels of mucosal leptin may interact with mast cells and the nervous system to contribute to the pathogenesis of IBS-D.

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“…A summary of recent findings is provided in Table 2 [ [34][35][36][37][38][39][40][41][42][43]44 & ]. A summary of recent findings is provided in Table 2 [ [34][35][36][37][38][39][40][41][42][43]44 & ].…”

Section: Neurotransmission and Neuromodulationmentioning

confidence: 99%

Colorectal sensation and motility

Gudsoorkar

Quigley²

2014

Current Opinion in Gastroenterology

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Leptin modulates enteric neurotransmission in the rat proximal colon: An in vitro study

Cited by 13 publications

References 57 publications

Adiponectin affects the mechanical responses in strips from the mouse gastric fundus

Adiponectin affects the mechanical responses in strips from the mouse gastric fundus

Increased intestinal mucosal leptin levels in patients with diarrhea-predominant irritable bowel syndrome

Colorectal sensation and motility

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