Leptin Expression in the Testicular Tissue of Fertile and Infertile Men

Soyupek, Sedat; Armağan, Abdullah; Ta, Serel; Mb, Hoşcan; Perk, Hakkı; Karaöz, Erdal; Çandir, Özden

doi:10.1080/01485010590919666

Cited by 33 publications

(19 citation statements)

References 25 publications

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“…In contrast, in the mouse, leptin and its receptor were detected exclusively in germ cells of immature and mature testes (El-Hefnawy et al, 2000;Herrid et al, 2008). Furthermore, in the human adult testes, leptin was found in seminiferous tubules, whereas Ob-R was detected in Leydig cells (Glander et al, 2002;Soyupek et al, 2005;Ishikawa et al, 2007).…”

Section: Discussionmentioning

confidence: 86%

“…In fact, Lep and/or Ob-R were detected in rodent testes (Caprio et al, 2003;El-Hefnawy et al, 2000;Herrid et al, 2008), in human testes and seminal plasma (Glander et al, 2002;Soyupek et al, 2005;Ishikawa et al, 2007), in human sperm (Aquila et al, 2005), in boar sperm (De Ambrogi et al, 2007), and in pig sperm (Aquila et al, 2008). Furthermore, a recent paper reported the effects of leptin treatment, alone or in combination with dietary energy restriction, on different reproductive parameters of male rats (Sirotkin et al, 2008).…”

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confidence: 99%

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Leptin and Its Receptor Are Expressed in the Testis and in the Epididymis of Young and Adult Pigs

Rago

Aquila

Guido

et al. 2009

The Anatomical Record

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Recent studies indicated that leptin, a 16 kDa hormone, is a regulatory signal in human and rodent male reproduction. This work was designed to investigate the expression of leptin and its receptor in testes and epididymides from immature and mature pigs. Immunolocalization revealed that leptin and its receptor were confined only in the interstitial compartment of immature testes, whereas both proteins were detected in Leydig cells and within seminiferous tubules of mature gonads. The immunostaining of epididymal tissues showed that leptin was absent in the epithelial cells of immature pigs but it was present in all the three regions of mature epididymides, although with a minor signal in the cauda. Conversely, leptin receptor was observed in all the epithelial cells of both immature and mature epididymides. Western blot analysis of tissue extracts detected a 16 kDa band for leptin and five/six isoforms, ranging from 120 to 40 kDa, for leptin receptor. In conclusion, this work has identified, for the first time, leptin and leptin receptor in the testis and in the epididymis of the pig showing a differential cell-type expression pattern of the two proteins in young and adult animals. Therefore, our findings suggest a possible involvement of leptin in endocrine or autocrine/ paracrine control of porcine male reproductive structures. Anat Rec, 292:736-745, 2009. V V C 2009 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 86%

mentioning

confidence: 99%

Leptin and Its Receptor Are Expressed in the Testis and in the Epididymis of Young and Adult Pigs

Rago

Aquila

Guido

et al. 2009

The Anatomical Record

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“…This discrepancy may be due to interspecies difference. LEP and OBR were found in human seminiferous tubules (Soyupek et al 2005), human seminal plasma (Jope et al 2003), human sperm and, recently, OBR was also identified in boar sperm (De Ambrogi et al 2007). In the present study, we investigated LEP and OBR expression in pig sperm, evaluating their potential role on sperm capacitation and survival.…”

Section: Introductionmentioning

confidence: 99%

Leptin and leptin receptor in pig spermatozoa: evidence of their involvement in sperm capacitation and survival

Aquila¹,

Rago²,

Guido³

et al. 2008

Reproduction

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Several studies have recently investigated the role of leptin, the adipocyte-secreted hormone, in the growth and reproduction of rodents, humans, and domestic animals. The present study was designed to explore the expression of leptin and its receptor in pig spermatozoa. Successful Western blot evidenced a 16 kDa band for leptin and six isoforms, ranging from 120 to 40 kDa, for the leptin receptor. Both leptin and leptin receptor were interestingly located at sperm acrosomal level, suggesting their involvement in the oocyte fertilization events. In fact, both capacitation indexes and acrosin activity were enhanced by leptin, and these effects were reduced by the anti-leptin receptor antibody. Afterwards, we investigated the main transduction pathways regulated by the hormone. Our results showed that, in pig sperm, leptin can trigger the signal transducer and activator of transcription 3, a classical component of cytokine signal transduction pathways, whose expression has not been previously reported in male gamete; in addition it was found constitutively activated. Besides, leptin was able to induce the activation of phosphatidylinositol phosphate kinase 3 and MAP kinase pathways as well as of BCL2, a known antiapoptotic protein. These data address to a role of leptin and its receptor on pig sperm survival. The presence of leptin and its receptor in pig sperm suggests that they, through an autocrine short loop, may induce signal transduction and molecular changes associated with sperm capacitation and survival. Reproduction (2008) 136 23-32

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“…It has central roles in the energy expenditure, food intake, many reproductive processes, regulation of energy homeostasis, neuroendocrine function, and metabolism (Kelesidis et al, 2010). Beside the synthesis by adipose tissue as the main source (Go et al, 2013), there have been indentified other sources of leptin in the body including testicles (Soyupek et al, 2005), ovaries (Löffler et al, 2001), placenta (Maymó et al, 2011), cartilage and bone cells (Morroni et al, 2004), skeletal muscle (Solberg et al, 2005) and stomach (Mix et al, 2004). Furthermore, the mitogenic, transforming or migration-induced properties of leptin have been revealed in many different cell types such as smooth muscle cells (Oda et al, 2001), normal and neoplastic colon cells (Hardwick et al, 2001;Liu et al, 2001); and also normal and malignant mammary epithelial cells (Dieudonne et al, 2002;Laud et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Leptin and Leptin Receptor Gene Expression by Silibinin-Curcumin Combination

Nejati-Koshki¹,

Akbarzadeh²,

Pourhasan-Moghaddam³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Leptin and its receptor are involved in breast carcinogenesis as mitogenic factors. Therefore, they could be considered as targets for breast cancer therapy. Expression of the leptin receptor gene could be modulated by leptin secretion. Silibinin and curcumin are herbal compounds with anti-cancer activity against breast cancer. The aim of this study was to assess their potential to inhibit of expression of the leptin gene and its receptor and leptin secretion. Cytotoxic effects of the two agents on combination on T47D breast cancer cells was investigated by MTT assay test after 24h treatment. With different concentrations the levels of leptin, leptin receptor genes expression were measured by reverse-transcription real-time PCR. Amount of secreted leptin in the culture medium was determined by ELISA. Data were statistically analyzed by one-way ANOVA test. The silibinin and curcumin combination inhibited growth of T47D cells in a dose dependent manner. There were also significant difference between control and treated cells in leptin expression and the quantity of secreted leptin with a relative decrease in leptin receptor expression. In conclusion, these herbal compounds inhibit the expression and secretion of leptin and it could probably be used as drug candidates for breast cancer therapy through leptin targeting in the future.

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Leptin Expression in the Testicular Tissue of Fertile and Infertile Men

Cited by 33 publications

References 25 publications

Leptin and Its Receptor Are Expressed in the Testis and in the Epididymis of Young and Adult Pigs

Leptin and Its Receptor Are Expressed in the Testis and in the Epididymis of Young and Adult Pigs

Leptin and leptin receptor in pig spermatozoa: evidence of their involvement in sperm capacitation and survival

Inhibition of Leptin and Leptin Receptor Gene Expression by Silibinin-Curcumin Combination

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