Leptin and insulin in the seminal plasma of zebu bulls in peripuberty

Souza, Fernando Andrade; Martins, Jorge André Matias; Filho, Vicente Ribeiro do Vale; Andrade, Venício José de; Ferreira, Melisane Regina Lima; Emerick, Lucas Luz; Pinto, Pablo Fabricio Bruno; Leite, Ticiano Guimarães

doi:10.1590/s1516-35982012000400013

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…In the present study, it was observed a higher percentage of cells with the intact plasma membrane, intact acrosome, and high mitochondrial membrane potential (PIAIHP) when insulin (150 µUI/mL) was added to both extenders. Such responses to the insulin action can be assured, since the plasma membrane, acrosomal and metabolism are cytological targets (Souza et al, 2012). During semen cryopreservation, between 15º to 5º C, mostly membranes alterations occur, such as rupture and loss in the phospholipid arrangements.…”

Section: Pimentioning

confidence: 99%

“…The addition of IGF-I and insulin combined did not improve sperm quality after thawing in both Triladyl ® and Botubov ® extenders. It was expected that the association of these hormones would guarantee better cell preservation, as IGF-I acted on sperm motility and linear speed, through the connection with receptors present in sperm, and that insulin acted on metabolism (Souza et al, 2012). The associated groups showed a lower percentage of sperm motility and a lower number of intact cells.…”

Section: Pimentioning

confidence: 99%

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Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation

Pinto,

Souza,

Arruda

et al. 2023

Arq. Bras. Med. Vet. Zootec.

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This study aimed to evaluate the addition of different concentrations of IGF-I and insulin to egg yolk-based extender to improve bovine semen cryopreservation. Two experiments were developed to evaluate the effects of the additives in two commercial extenders, Botubov® (Experiment 1) and Triladyl® (Experiment 2), both with the same design. Three ejaculates from four bulls (n = 12) were used. Each ejaculate was divided into seven equal fractions for dilution (60x106 spermatozoa/mL) in the following treatments: CON: extender only; IGF100: IGF-I 100ng/mL; IGF200: IGF-I 200ng/mL; INS150: insulin 150µUI/mL; INS200: insulin 200µUI/mL; ASS1: IGF-I 100ng/mL + insulin 150µUI/mL; ASS2: IGF-I 200ng/mL + insulin 200µUI/mL. Semen was cryopreserved by an automated system. Post-thawed sperm were evaluated regarding motility by CASA (Computer-assisted sperm analysis), and membranes by fluorescent probes (H342, PI, FITC-PSA and JC-1). For Botubov® extender, INS150 was more efficient in preserving total and progressive motility, VCL, BCF, plasma and mitochondrial membranes. A similar response was seen when insulin was added to the Triladyl® extender, INS150 was more efficient in preserving sperm motility, plasma membrane integrity and mitochondrial potential. Thus, the addition of insulin 150µUI/mL, regardless of the composition of the extender, contributes to better preserving bovine sperm from the cryopreservation effects.

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Section: Pimentioning

confidence: 99%

Section: Pimentioning

confidence: 99%

Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation

Pinto,

Souza,

Arruda

et al. 2023

Arq. Bras. Med. Vet. Zootec.

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“…As a pleiotropic molecule, leptin exhibits both positive and negative influences on sperm quality ( Andò & Aquila, 2005 ; Leisegang et al, 2014 ; Xin et al, 2019 ). The evidence has indicated considerable differences as to the concentrations of leptin in seminal plasma of different species ( Lackey, Gray & Henricks, 2002 ; Guo et al, 2014 ; Elfassy et al, 2018 ; El-Maaty et al, 2014 ; Kumar et al, 2016 ; SouzaI et al, 2012 ), the physiological implications of these differences in seminal plasma have not been clarified.…”

Section: Introductionmentioning

confidence: 99%

Presence of leptin and its receptor in the ram reproductive system and in vitro effect of leptin on sperm quality

Gao

Zhao

Song

et al. 2022

PeerJ

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Leptin is a 16 kDa hormone encoded by obese (OB) gene in adipocytes. This molecule not only regulates energy metabolism but also plays a role in the reproduction of mammals. Leptin and its receptor (OBR) have been found in male reproductive systems of human, bovine, equine and pig. The effects of leptin on sperm quality vary widely from different research findings. However, the presence of leptin and its receptor in the ram reproductive system and the in vitro effect of leptin on sperm quality have not reported yet. In the present study, we found that the OB was highly expressed in primary and secondary spermatocytes of the testes, OBR was highly expressed in secondary spermatocytes of the testes. The expressions of OB were in stereocilia of epididymis and in columnar cells of epididymal caput and cauda, the expressions of OBR were in columnar cells of epididymis and in stereocilia of epididymal corpus and cauda. The presence of both OB and OBR in testes, epididymis and sperm were confirmed through RT-PCR, immunolocalization and Western blot analyses. The RT-qPCR results indicated OB and OBR had higher expression levels in epididymal sperm than that of the ejaculated sperm in rams. When sperm were treated with 5 ng/mL leptin, the progressive motility (P < 0.01), straight-line velocity (VSL) (P < 0.05), average path velocity (VAP) (P < 0.05), membrane mitochondrial potential (MMP) (P < 0.01) and viability (P < 0.05) significantly increased, while DNA fragmentation index (DFI) and reactive oxygen species (ROS) significantly decreased compared to the control (P < 0.01), and the other semen parameters such as acrosome integrity and acrosome reaction rate had no significant changes between groups (P > 0.05). In conclusion, this is probably the first report describing localization of leptin and its receptors in the reproductive system of rams and their effects on sperm quality parameters. Our findings suggest that 5 ng/mL leptin treatment enhanced sperm motility, viability and MMP, and decrease DFI and ROS without obvious influence on the acrosome reaction in ram sperm. The potential mechanisms may be related to leptin’s ability to reduce the oxidative stress and apoptosis of sperms and improve their mitochondrial function and energy supply, therefore, to maintain the physiological homeostasis of the sperm.

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Quantification of leptin in seminal plasma of buffalo bulls and its correlation with antioxidant status, conventional and computer-assisted sperm analysis (CASA) semen variables

Kumar

Saini

Kumar

et al. 2016

Animal Reproduction Science

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Leptin and insulin in the seminal plasma of zebu bulls in peripuberty

Cited by 5 publications

References 22 publications

Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation

Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation

Presence of leptin and its receptor in the ram reproductive system and in vitro effect of leptin on sperm quality

Quantification of leptin in seminal plasma of buffalo bulls and its correlation with antioxidant status, conventional and computer-assisted sperm analysis (CASA) semen variables

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