Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation
S.C.C. Pinto,
F.A. Souza,
R.P. Arruda
et al.
Abstract:This study aimed to evaluate the addition of different concentrations of IGF-I and insulin to egg yolk-based extender to improve bovine semen cryopreservation. Two experiments were developed to evaluate the effects of the additives in two commercial extenders, Botubov® (Experiment 1) and Triladyl® (Experiment 2), both with the same design. Three ejaculates from four bulls (n = 12) were used. Each ejaculate was divided into seven equal fractions for dilution (60x106 spermatozoa/mL) in the following treatments: … Show more
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