Lentivirus-Mediated RNA Interference Targeting the Long Noncoding RNA HOTAIR Inhibits Proliferation and Invasion of Endometrial Carcinoma Cells In Vitro and In Vivo

Huang, Jiaming; Ke, Peiqi; Guo, Linjie; Wang, Wei; Tan, Hao; Liang, Y. Daniel; Yao, Shuzhong

doi:10.1097/igc.0000000000000121

Cited by 68 publications

(59 citation statements)

References 26 publications

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“…Knockdown of HOTAIR suppressed the tumorigenicity of HeLa cells, as measured by tumor mass (Fig. 5J), consistent with previous functional studies in pancreatic cancer cells (23,45,46). Western blotting subsequently verified the up-regulation of VIM in HOTAIR knockdown xenografts compared with control xenografts (Fig.…”

Section: Validation Of the Quantitative Proteomics Data-supporting

confidence: 74%

Quantitative Proteomics Analysis Reveals Novel Insights into Mechanisms of Action of Long Noncoding RNA Hox Transcript Antisense Intergenic RNA (HOTAIR) in HeLa Cells*

Zheng

Xiong

et al. 2015

Molecular & Cellular Proteomics

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Section: Validation Of the Quantitative Proteomics Data-supporting

confidence: 74%

Quantitative Proteomics Analysis Reveals Novel Insights into Mechanisms of Action of Long Noncoding RNA Hox Transcript Antisense Intergenic RNA (HOTAIR) in HeLa Cells*

Zheng

Xiong

et al. 2015

Molecular & Cellular Proteomics

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“…In endometrial carcinoma, the overexpression of HOTAIR increases the malignant potential of tumor cells in vitro and in vivo (24). The knockdown of HOTAIR lncRNA suppresses tumor invasion in gastric cancer cells and reverses the epithelial-mesenchymal transition (38).…”

Section: Discussionmentioning

confidence: 99%

“…The 3' end of HOTAIR interacts with lysine-specific histone demethylase 1A (22). Furthermore, recent studies have reported that HOTAIR is highly expressed and associated with poor prognosis in a number of types of cancer, including breast cancer, epithelial ovarian cancer, pancreatic cancer, colorectal cancer, non-small-cell lung cancer and endometrial carcinoma (21,(23)(24)(25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence

Jianzhi

et al. 2016

Oncology Letters

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Abstract. The present study aimed to investigate the expression level of HOX transcript antisense RNA (HOTAIR) in hepatocellular carcinoma (HCC) and its association with various clinicopathological characteristics, and to further explore the molecular mechanisms of HOTAIR function in HCC. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression level of HOTAIR in 60 paired fresh HCC samples and adjacent normal liver tissue samples. The association between HOTAIR expression and clinicopathological parameters was analyzed. Lentivirus-mediated HOTAIR-specific small hairpin RNA vectors were transfected into HepG2 cells. Cell proliferation and invasion in vitro were examined by MTT and Transwell assays, respectively. A xenograft model was used to analyze the tumorigenesis of liver cancer cells in vivo. In addition, semi-quantitative RT-PCR was used to detect the expression level of Wnt/β-catenin signaling molecules under the condition of HOTAIR inhibition. The results revealed that the expression level of HOTAIR in HCC tissues was higher than that in adjacent non-cancerous tissues. HOTAIR expression was significantly associated with poor tumor differentiation (P=0.002), metastasis (P=0.002) and early recurrence (P=0.001). In vitro, the inhibition of HOTAIR in liver cancer cells resulted in the suppression of cell proliferation and invasion. HOTAIR depletion significantly inhibited the rate of growth of liver cancer cells in vivo. Furthermore, the expression levels of Wnt and β-catenin were downregulated when HOTAIR expression was suppressed. In conclusion, HOTAIR is important in the progression and recurrence of HCC, partly through the regulation of the Wnt/β-catenin signaling pathway. Targeting HOTAIR may be a novel therapeutic strategy for HCC.

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“…In addition, a significant association between HOTAIR expression and the EC grade and lymph node metastasis suggested that HOTAIR could be used as a novel prognostic biomarker in patients with EC [111]. Depletion of HOTAIR in vivo significantly suppressed endometrial tumorigenesis and reduced the size of tumors [52,111,112]. Knockdown of HOTAIR substantially inhibited proliferation, migration, invasion, and remarkable G0/G1 phase arrest in EC cells.…”

Section: Hotair In Endometrial Carcinomamentioning

confidence: 99%

“…HOTAIR expression in EC tissues was significantly increased relative to that in normal tissues, and high HOTAIR expression was positively correlated with metastasis and shortened OS in EC. In addition, a significant association between HOTAIR expression and the EC grade and lymph node metastasis suggested that HOTAIR could be used as a novel prognostic biomarker in patients with EC [111]. Depletion of HOTAIR in vivo significantly suppressed endometrial tumorigenesis and reduced the size of tumors [52,111,112].…”

Section: Hotair In Endometrial Carcinomamentioning

confidence: 99%

HOTAIR: An Oncogenic Long Non-Coding RNA in Human Cancer

Tang

Hann

2018

Cell Physiol Biochem

204

127

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Long non-coding RNAs (LncRNAs) represent a novel class of noncoding RNAs that are longer than 200 nucleotides without protein-coding potential and function as novel master regulators in various human diseases, including cancer. Accumulating evidence shows that lncRNAs are dysregulated and implicated in various aspects of cellular homeostasis, such as proliferation, apoptosis, mobility, invasion, metastasis, chromatin remodeling, gene transcription, and post-transcriptional processing. However, the mechanisms by which lncRNAs regulate various biological functions in human diseases have yet to be determined. HOX antisense intergenic RNA (HOTAIR) is a recently discovered lncRNA and plays a critical role in various areas of cancer, such as proliferation, survival, migration, drug resistance, and genomic stability. In this review, we briefly introduce the concept, identification, and biological functions of HOTAIR. We then describe the involvement of HOTAIR that has been associated with tumorigenesis, growth, invasion, cancer stem cell differentiation, metastasis, and drug resistance in cancer. We also discuss emerging insights into the role of HOTAIR as potential biomarkers and therapeutic targets for novel treatment paradigms in cancer.

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Lentivirus-Mediated RNA Interference Targeting the Long Noncoding RNA HOTAIR Inhibits Proliferation and Invasion of Endometrial Carcinoma Cells In Vitro and In Vivo

Abstract: This study is the first to suggest that HOTAIR plays an important role in the carcinogenesis of endometrial cancer. Targeting HOTAIR may be a novel therapeutic strategy for endometrial cancer.

Cited by 68 publications

References 26 publications

Quantitative Proteomics Analysis Reveals Novel Insights into Mechanisms of Action of Long Noncoding RNA Hox Transcript Antisense Intergenic RNA (HOTAIR) in HeLa Cells*

Quantitative Proteomics Analysis Reveals Novel Insights into Mechanisms of Action of Long Noncoding RNA Hox Transcript Antisense Intergenic RNA (HOTAIR) in HeLa Cells*

Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence

HOTAIR: An Oncogenic Long Non-Coding RNA in Human Cancer

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