Lentinan inhibited colon cancer growth by inducing endoplasmic reticulum stress-mediated autophagic cell death and apoptosis

Zhang, Yu; Liu, Yan; Zhou, Yinxing; Zheng, Ziming; Tang, Wenqi; Song, Min; Wang, Jinglin; Wang, Kaiping

doi:10.1016/j.carbpol.2021.118154

Cited by 45 publications

(29 citation statements)

References 50 publications

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“…In this study, the survival rate of mice was 100% after a single intravenous dose of 8 mg/kg. In our previous study, we found that no mice were died after the continuous intravenous dose of 5 mg/kg for 21 days (11). These data confirmed that LNT was safe, consistent with reported safety of other β-glucans (29).…”

Section: Toxicity Of Flnt In Vivosupporting

confidence: 88%

“…T-series Dextrans (1-2000 kDa) were used to determine the standard curve, and the Mw of LNT was calculated with the Agilent HPGPC software (Agilent, USA) according to the calibration curve equation. The ultraviolet (UV) and Fourier transform infrared (FT-IR) spectra analyses were performed according to our previous studies (11,25). Brie?y, the UV spectrum of the LNT solution (1 mg/ml) was recorded with a UV spectrophotometer in the region of 200-800 nm for detecting proteins and nucleic acids.…”

Section: Extraction and Characterization Of Lntmentioning

confidence: 99%

“…LNT, a natural extract product with a unique advantage of multiple pharmacological activities and less toxicities, has attracted more and more concerns. However, the main studies focused on structure characterization, clinical effects, immune mechanisms, and new bioactivities (7)(8)(9)(10)(11), the pharmacokinetics in vivo were rarely reported. Furthermore, the pharmacokinetics information in the instruction of LNT injection, which has been approved for listing and used in clinical, was described incompletely.…”

Section: Introductionmentioning

confidence: 99%

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Metabolism and Biodegradation of β-Glucan in vivo

Zheng

Tang

et al. 2022

Front. Vet. Sci.

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The β-Glucans widely exist in plants and edible fungi, and their diverse bioactivities and good physicochemical properties have been widely reported. In addition, β-glucan intravenous injections (such as lentinan and schizophyllan) have been clinically used as immunomodulators and antitumor polysaccharides. However, the pharmacokinetic studies of β-glucans only stay on the level of plasma concentration and biodistribution in vivo, and little is known about their metabolism and degradation in vivo, which severely limits the further application of β-glucans in the field of medicine and biomaterials. The aim of this paper is to explore the metabolism and degradation process of lentinan (as a representative of β-glucans) in vivo by labeling it with water-soluble fluorescein 5-([4, 6-Dichlorotriazin-2-yl]amino)fluorescein (DTAF). Fluorescently labeled lentinan (FLNT) was intravenously administered to rats at a single dose of 8 mg/kg. The degradation of LNT in blood, liver, kidney, and urine was evaluated by the gel permeation chromatography. Our results showed that although LNT could be degraded in blood, liver, kidney, and urine, there were still some prototypes until excreted in urine due to the incomplete degradation of LNT in each step. To the best of our knowledge, this is the first report to comprehensively study LNT metabolic degradation in rats. These results provide an important reference for further exploration and application of LNT and other β-glucans.

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Section: Toxicity Of Flnt In Vivosupporting

confidence: 88%

Section: Extraction and Characterization Of Lntmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolism and Biodegradation of β-Glucan in vivo

Zheng

Tang

et al. 2022

Front. Vet. Sci.

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“…However, when ER stress is severe and induces cell death, divert results will work out. Many studies in the cell level targeted for colon cancer treatment pointed out that cell death induced by ER stress may target antitumor medicine ( Tung et al, 2015 ; Dilly et al, 2020 ; Zhang et al, 2021 ).…”

Section: Link Of Intestinal Disease and Er Stressmentioning

confidence: 99%

Endoplasmic Reticulum Stress of Gut Enterocyte and Intestinal Diseases

Gao

Hua

et al. 2022

Front. Mol. Biosci.

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The endoplasmic reticulum, a vast reticular membranous network from the nuclear envelope to the plasma membrane responsible for the synthesis, maturation, and trafficking of a wide range of proteins, is considerably sensitive to changes in its luminal homeostasis. The loss of ER luminal homeostasis leads to abnormalities referred to as endoplasmic reticulum (ER) stress. Thus, the cell activates an adaptive response known as the unfolded protein response (UPR), a mechanism to stabilize ER homeostasis under severe environmental conditions. ER stress has recently been postulated as a disease research breakthrough due to its significant role in multiple vital cellular functions. This has caused numerous reports that ER stress-induced cell dysfunction has been implicated as an essential contributor to the occurrence and development of many diseases, resulting in them targeting the relief of ER stress. This review aims to outline the multiple molecular mechanisms of ER stress that can elucidate ER as an expansive, membrane-enclosed organelle playing a crucial role in numerous cellular functions with evident changes of several cells encountering ER stress. Alongside, we mainly focused on the therapeutic potential of ER stress inhibition in gastrointestinal diseases such as inflammatory bowel disease (IBD) and colorectal cancer. To conclude, we reviewed advanced research and highlighted future treatment strategies of ER stress-associated conditions.

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“…Lentinan primarily possesses an immune enhancement effect in tumor patients, as well as the immunomodulatory properties [49,50]. Polysaccharides extracted from L. edodes have not only the enhancement effect, but may also inhibit tumor growth through various mechanisms, such as inducing tumor cell apoptosis and directly killing tumor cells [51,52]. Lentinan is considered to be one of the most active components in mushrooms (L. edodes).…”

Section: Mushroom Beta-glucansmentioning

confidence: 99%

Beta-Glucans from Fungi: Biological and Health-Promoting Potential in the COVID-19 Pandemic Era

Mirończuk-Chodakowska

Kujawowicz

Witkowska

2021

Nutrients

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Beta-glucans comprise a group of polysaccharides of natural origin found in bacteria, algae, and plants, e.g., cereal seeds, as well as microfungi and macrofungi (mushrooms), which are characterized by diverse structures and functions. They are known for their metabolic and immunomodulatory properties, including anticancer, antibacterial, and antiviral. Recent reports suggest a potential of beta-glucans in the prevention and treatment of COVID-19. In contrast to β-glucans from other sources, β-glucans from mushrooms are characterized by β-1,3-glucans with short β-1,6-side chains. This structure is recognized by receptors located on the surface of immune cells; thus, mushroom β-glucans have specific immunomodulatory properties and gained BRM (biological response modifier) status. Moreover, mushroom beta-glucans also owe their properties to the formation of triple helix conformation, which is one of the key factors influencing the bioactivity of mushroom beta-glucans. This review summarizes the latest findings on biological and health-promoting potential of mushroom beta-glucans for the treatment of civilization and viral diseases, with particular emphasis on COVID-19.

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Lentinan inhibited colon cancer growth by inducing endoplasmic reticulum stress-mediated autophagic cell death and apoptosis

Cited by 45 publications

References 50 publications

Metabolism and Biodegradation of β-Glucan in vivo

Metabolism and Biodegradation of β-Glucan in vivo

Endoplasmic Reticulum Stress of Gut Enterocyte and Intestinal Diseases

Beta-Glucans from Fungi: Biological and Health-Promoting Potential in the COVID-19 Pandemic Era

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