Lentinan extracted from shiitake mushrooms (Lentinus edodes) improves the non-specific immunity of sea cucumber (Apostichopus japonicus)

Wang, Yiyan; Wang, Haina; Yan, Dawei; Wang, Lei; Zhenxing, Sun; Sun, Hushan

doi:10.1007/s10499-013-9628-7

Cited by 9 publications

(9 citation statements)

References 42 publications

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“…HPLC-MS/MS analyses of each sample were performed on a Perkin Elmer UHPLC system with a OD-300 Aquapore column. The elution gradient used was based on (A) [22].…”

Section: Hplc-ms/ms Analysismentioning

confidence: 99%

“…In the shiitake mushroom, one of the most relevant is lentinan, a β-glucan compound demonstarting several activities that comprise the antitumor effect due to induced stimulation of the host immune system [6]. Other interesting metabolites are ergothioneine, showing antioxidant activity and eritadenine, which holds an hypocholesterolemic effect [22], [23], [24].…”

Section: Introductionmentioning

confidence: 99%

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Lentinula edodes and Pleurotus ostreatus: functional food with antioxidant - antimicrobial activity and an important source of Vitamin D and medicinal compounds

Parola¹,

Chiodaroli²,

Orlandi³

et al. 2017

FFHD

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Background: Mushrooms produce a large amount of medicinal compounds, and are also an optimal source of fibres, proteins, vitamins (like groups B and D), and other micronutrients including potassium, magnesium, etc. Consequently, mushrooms are commonly considered to be functional foods. Many works report the high biological potentials of medicinal mushrooms involving their antibacterial, hypoglycaemic, anticholesterolemic, radical scavenging, and anti-inflammatory effects.Context and purpose of this study: First off, this work aimed to find strains of Lentinula edodes and Pleurotus ostreatus from a bank of edible mushrooms bought from international strain banks (Table I) that could possess health benefit related properties, such as a radical scavenging activity (antioxidant effect), antibacterial effects against common pathogenic bacteria, and being able to produce interesting nutrients and secondary metabolites. As the fungal bank comprises of 20 strains of L. edodes and 20 strains of P. ostreatus, a first screening was made by the selection of 13 strains for each mushroom able to grow in multiple wood types or that were particularly productive and had proved good growth reproducibility over the last 5 years. This work also studied the correlation between culture conditions and mushroom quality in terms of the previously reported properties. Comparison among the selected strains was operated by the assessment of antioxidant and antimicrobial activities after different sample treatments. Furthermore, an initial optimization of the analytic techniques was produced for the direct estimation of important secondary metabolites and nutrients by means of HPLC-MS/MS technique. Further research will encompass an evaluation of transformation processes (drying, freezing, rehydration, cooking, etc.) impact on radical scavenging, antibacterial activity, and possible degradation/loss of nutraceutically important substances such as vitamin D2, ergothioneine, eritadenine, lovastatin, lentinan, and lenthionine. Results: 13 strains of each mushroom species have been cultivated on different wood logs. Seven strains of shiitake and six strains of oyster mushroom were able to produce sporocarps. Antioxidant levels in water extracts from dried mushrooms produced significatively different results on the basis of strains and of wood. Both mushrooms demonstrated higher radical scavenging activity in log cultivation than substrates cultivation, which was subsequently used as reference. Furthermore, all strains of P. ostreatus demonstrated the lowest level of antioxidant activity at 4°C, a significant increase towards 50°C and a limited decrease towards 80°C. The same trend was observed for shiitake extracts. Concerning the shiitake mushroom only, crude water extracts showed an interesting antibacterial activity against the model microorganisms Pseudomonas aeruginosa and Staphylococcus aureus. A comparison was also performed between the best performing strain extract and the commercial antibiotic Ceftriaxone against P. aeruginosa, assessing that 20 mg of crude extract corresponds to 0.2 mg of the pure antibiotic when studied by means of disk diffusion assay.Conclusions: The results suggested that the cultivation of both shiitake and oyster mushrooms on logs could enhance the content of antioxidant and antibacterial activities, compared to the cultivation of mushrooms on sawdust substrates. Radical scavenging and antibacterial activity depends both on L. edodes strain and the log type. The bacteriostatic/bactericidal activity of the best performer strain may depend on a pH and solvent treatment sensitive substance. Secondary metabolites such as ergothioneine and vitamin D2 from both shiitake and oyster were released just after water extraction: this suggests that the transformation/cooking processes may produce a loss of characteristic mushroom biological properties in water. Further evaluation of biologically relevant compounds content and loss during different food transformation and cooking processes will be assessed.Key words: Shiitake, Oyster, Pleurotus ostreatus, Lentinula edodes, Pseudomonas aeruginosa, Staphylococcus aureus, Log grown mushroom, antioxidant, radical scavenging, total phenolic content, DPPH, ABTS, Folin-Ciocalteu, antibacterial, fruitbodies, sporocarps.

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“…HPLC-MS/MS analyses of each sample were performed on a Perkin Elmer UHPLC system with a OD-300 Aquapore column. The elution gradient used was based on (A) [22].…”

Section: Hplc-ms/ms Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lentinula edodes and Pleurotus ostreatus: functional food with antioxidant - antimicrobial activity and an important source of Vitamin D and medicinal compounds

Parola¹,

Chiodaroli²,

Orlandi³

et al. 2017

FFHD

View full text Add to dashboard Cite

show abstract

“…Supplemenatry Files (1)(2)(3)(4) showed the MS/MS spectra of different lentinan concentrations at the column and buffer combinations that generated optimum MS/MS spectra in previous analysis. Each lentinan concentration showed a peak of 1151.3731.…”

Section: Dmentioning

confidence: 99%

“…Lentinan, a polysaccharide β-D-glucan, is widely used as an alternative medicine and also as dietary supplement nowadays as it claimed to have a lot of health benefits such as antimicrobial, antitumor, hypocholesterolemic actions and antioxidants. It was detected for years in a well-known edible mushroom namely Shiitake Mushroom (Lentinus edodes) [1][2][3][4]. This compound has been known as Biology Response Modifier (BRM) as it is actively mediates anticancer activity through activation of human immune system [1,[5][6][7].…”

Section: Introductionmentioning

confidence: 99%

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Liquid Chromatography MS/MS Responses on Lentinan for Structure Characterization of Mushroom Polysaccharide β-D-Glucan

Jami¹

2014

J Chromatogr Sep Tech

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Lentinan, a complex polysaccharides β-D-glucan found in Shiitake Mushroom has been known to have medicinal effect such as anticancer and immune-modulatory properties. Analysis on lentinan through application of high-end biotechnology instrument such as mass spectrometry is still limited. In this study, Liquid Chromatography Mass Spectrometry-Quadropole Time of Flight (LC/MS Q-TOF) has been used to optimize the MS/MS response in elucidating the lentinan characteristics. Analysis was done by using lentinan standard at different concentrations through different combinations of four types of columns and four types of buffers. Four levels of MS/MS collision energies or known as Collision Induced Dissociation (CID) were applied. Data presented shown that the optimum MS/MS CID that generated all six product ion of lentinan at the most combinations of column and buffer was at 20.0 V. Combination of Altima Hydrophilic Interaction Liquid Chromatography (HILIC) column without any buffer in mobile phase (AH; NB) and Hypercarb column with the present of 0.1% acetic acid in mobile phase (TH; 0.1% AA) respectively were found to be the best combinations among other combinations as these combinations were able to generate product ions of lentinan at lentinan concentration as low as 0.156 mg/mL. This suggested that these column and buffer combinations have given good sensitivity to enhance peak efficiency of lentinan at low concentration which was required to meet MS/MS abundance threshold. As the conclusion, suitable combinations of column and buffer are crucial in order to enhance peak efficiency of lentinan at optimum ionization intensity to allow optimum MS/MS responses for elucidation of their structure characteristics. Determination of appropriate collision energy also plays an important role to generate MS/MS spectra of all product ions for the lentinan.

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Chemical substances and their activities in sea cucumber Apostichopus japonicus: A review

Lu,

Wang,

et al. 2023

Archiv der Pharmazie

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Apostichopus japonicus, also known as Stichopus japonicus, with medicinal and food homologous figures, is a globally recognized precious ingredient with extremely high nutritional value. There is no relevant review available through literature search, so this article selects the research articles through the keywords “sea cucumber” and “Apostichopus japonicus (Stichopus japonicus)” in six professional databases, such as Wiley, PubMed, ScienceDirect, ACS, Springer, and Web of Science, from 2000 to the present, summarizing the extraction, isolation, and purification methods for the four major categories (polysaccharides, proteins and peptides, saponins, and other components) of the A. japonicus chemical substances and 10 effective biological activities of A. japonicus. Included are anticoagulation, anticancer/antitumor activities, hematopoiesis, regulation of gut microbiota, and immune regulatory activities that correspond to traditional efficacy. Literature support is provided for the development of medicines and functional foods and related aspects that play a leading role in future directions.

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Lentinan extracted from shiitake mushrooms (Lentinus edodes) improves the non-specific immunity of sea cucumber (Apostichopus japonicus)

Cited by 9 publications

References 42 publications

Lentinula edodes and Pleurotus ostreatus: functional food with antioxidant - antimicrobial activity and an important source of Vitamin D and medicinal compounds

Lentinula edodes and Pleurotus ostreatus: functional food with antioxidant - antimicrobial activity and an important source of Vitamin D and medicinal compounds

Liquid Chromatography MS/MS Responses on Lentinan for Structure Characterization of Mushroom Polysaccharide β-D-Glucan

Chemical substances and their activities in sea cucumber Apostichopus japonicus: A review

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