Lemon exosome-like nanoparticles enhance stress survival of gut bacteria by RNase P-mediated specific tRNA decay

Abstract

Diet and bile play critical roles in shaping gut microbiota, but the molecular mechanism underlying interplay with intestinal microbiota is unclear. Here, we showed that lemon-derived exosome-like nanoparticles (LELNs) enhance lactobacilli toleration to bile. To decipher the mechanism, we used Lactobacillus rhamnosus GG (LGG) as proof of concept to show that LELNs enhance LGG bile resistance via limiting production of Msp1 and Msp3, resulting in decrease of bile accessibility to cell membrane. Furthermore, we found that decline of Msps protein levels was regulated through specific tRNA ser UCC and tRNA ser UCG decay. We identified RNase P, an essential housekeeping endonuclease, being responsible for LELNsinduced tRNA ser UCC and tRNA ser UCG decay. We further identified galacturonic acid-enriched pectin-type polysaccharide as the active factor in LELNs to increase bile resistance and downregulate tRNA ser UCC and tRNA ser UCG level in the LGG. Our study demonstrates a tRNA-based gene expression regulation mechanism among lactobacilli to increase bile resistance.

Protective effect of nimbolide against streptozotocin induced gestational diabetes mellitus in rats via alteration of inflammatory reaction, oxidative stress, and gut microbiota

“…54 Increased levels of Clostridium bacteria boost the inflammatory reaction and oxidative stress in the circulation and suppression of Clostridium bacteria could suppress the production of harmful products from the intestine into the circulation and down-regulate the occurrence of inflammation and oxidative stress. 55 Fusicatenibacter and Clostrodium were positively correlated with FBC level. Both are favorably connected with oxidative stress and inflammatory response, while glucose metabolism and antioxidant enzyme activity are negatively correlated.…”

“…The increased level of Clostridium in the intestine precipitates inflammatory reactions in diabetics 54 . Increased levels of Clostridium bacteria boost the inflammatory reaction and oxidative stress in the circulation and suppression of Clostridium bacteria could suppress the production of harmful products from the intestine into the circulation and down‐regulate the occurrence of inflammation and oxidative stress 55 . Fusicatenibacter and Clostrodium were positively correlated with FBC level.…”

The Emerging Role of Plant-Derived Exosomes-Like Nanoparticles in Immune Regulation and Periodontitis Treatment

“…Besides, PELNs show microbiome regulatory properties through the promotion of the growth of probiotics. Lemon-derived ELNs protect LGG from bile possibly through inhibition of the expression of Msps protein, induction of specific bacterial tRNA decay, and inhibition of S.24-7 growth (93). Lemon-derived ELNs…”

“…(B) Modulate microbiota: PELNs inhibit pathogenic bacteria such as P. gingivalis through PA binding to HBP35 (19). PELNs protect probiotics through decay tRNA in probiotics (93). PELNs transform miRNA to microbiota, which has the potential to modulate oral microbiota.…”

Emerging Role of Edible Exosomes-Like Nanoparticles (ELNs) as Hepatoprotective Agents

“…At the same time they also possess miR7267-3p which helps in proliferation of LGG in gut. Whereas Lemon derived ELNs enhances bile resistance in LGG by limiting the expression of Msp1 or Msp2 genes and also manipulates Streptococcus thermophilus ST-21 (STH) mitigates Clostridioides difficile infections (CDI) which is a leading cause of antibiotic resistant colitis 50 , 51 . A pre- clinical study in diet- induced mice models has shown therapeutic aspects of Orange derived ELNs (OELNs) that mainly targets microsomal triglyceride transfer protein (MTP) and angiopoietin-like protein-4 (ANGPTL4) and reduces the lipid absorption as well as inflammation in gastrointestinal lining.…”