Leishmania (Viannia) Infection in the Domestic Dog in Chaparral, Colombia

Santaella, Julian; Ocampo, Clara B.; Saravia, Nancy Gore; Méndez, Fabián; Góngora, Rafael; Gómez, María Adelaida; Munstermann, Leonard E.; Quinnell, Rupert J.

doi:10.4269/ajtmh.2011.10-0159

Cited by 37 publications

(24 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To date, there is no solid evidence that dogs act as reservoir hosts for the domestic transmission of CL 92,99 . Most studies are designed to determine the prevalence of CL in dogs, however, little is known about the parasitologic and immunologic course of infection.…”

Section: Dog Modelmentioning

confidence: 99%

Animal Models for the Study of Leishmaniasis Immunology

Loría-Cervera

Andrade-Narvaez

2014

Rev. Inst. Med. trop. S. Paulo

131

101

View full text Add to dashboard Cite

Leishmaniasis remains a major public health problem worldwide and is classified as Category I by the TDR/WHO, mainly due to the absence of control. Many experimental models like rodents, dogs and monkeys have been developed, each with specific features, in order to characterize the immune response to Leishmania species, but none reproduces the pathology observed in human disease. Conflicting data may arise in part because different parasite strains or species are being examined, different tissue targets (mice footpad, ear, or base of tail) are being infected, and different numbers (“low” 1×102 and “high” 1×106) of metacyclic promastigotes have been inoculated. Recently, new approaches have been proposed to provide more meaningful data regarding the host response and pathogenesis that parallels human disease. The use of sand fly saliva and low numbers of parasites in experimental infections has led to mimic natural transmission and find new molecules and immune mechanisms which should be considered when designing vaccines and control strategies. Moreover, the use of wild rodents as experimental models has been proposed as a good alternative for studying the host-pathogen relationships and for testing candidate vaccines. To date, using natural reservoirs to study Leishmania infection has been challenging because immunologic reagents for use in wild rodents are lacking. This review discusses the principal immunological findings against Leishmania infection in different animal models highlighting the importance of using experimental conditions similar to natural transmission and reservoir species as experimental models to study the immunopathology of the disease.

show abstract

Section: Dog Modelmentioning

confidence: 99%

Animal Models for the Study of Leishmaniasis Immunology

Loría-Cervera

Andrade-Narvaez

2014

Rev. Inst. Med. trop. S. Paulo

131

101

View full text Add to dashboard Cite

show abstract

“…These results also confirm previous reports of this Leishmania species circulating in this outbreak in humans and dogs. 10,38 The presence of phlebotomine infected with Le. ( V. ) parasites in the post-epidemic period is surprising, because no active CL lesions were observed during these studies.…”

mentioning

confidence: 99%

Phlebotomine Vector Ecology in the Domestic Transmission of American Cutaneous Leishmaniasis in Chaparral, Colombia

Ferro¹,

Marín²,

Góngora³

et al. 2011

The American Society of Tropical Medicine and Hygiene

Self Cite

View full text Add to dashboard Cite

Phlebotomine vector ecology was studied in the largest recorded outbreak of American cutaneous leishmaniasis in Colombia in 2004. In two rural townships that had experienced contrasting patterns of case incidence, this study evaluated phlebotomine species composition, seasonal abundance, nocturnal activity, blood source, prevalence of Leishmania infection, and species identification. CDC miniature light traps were used to trap the phlebotomines. Traps were set indoors, peridomestically, and in woodlands. Natural infection was determined in pools by polymerase chain reaction–Southern blot, and blood sources and species identification were determined by sequencing. Large differences were observed in population abundance between the two townships evaluated. Lutzomyia longiflocosa was the most abundant species (83.1%). Abundance was higher during months with lower precipitation. Nocturnal activity was associated with human domestic activity. Blood sources identified were mainly human (85%). A high prevalence of infection was found in L. longiflocosa indoors (2.7%) and the peridomestic setting (2.5%). L. longiflocosa was responsible for domestic transmission in Chaparral.

show abstract

“…Furthermore, such animals may attract vectors to domestic sites, increasing the risk of infection of humans [11]. The species known to cause CL in dogs are L (V.) braziliensis, L (V.) panamensis, L (V.) guyanensis , and L (V.) peruviana [4, 20]. L (V.) braziliensis and L (V.) guyanensis are known to cause CL in Suriname and are present in high-transmission areas in the country [7, 8].…”

Section: Discussionmentioning

confidence: 99%

Is the Dog a Possible Reservoir for Cutaneous Leishmaniasis in Suriname?

Kent

Ramkalup²,

Mans

et al. 2013

Journal of Tropical Medicine

View full text Add to dashboard Cite

Cutaneous leishmaniasis (CL) is an emerging disease in Suriname, with at least 200 cases per year. Little is known about the biology of CL in the country. The most important parasite species is Leishmania Viannia guyanensis, but possible vectors and reservoirs are hardly incriminated. In the present study, it was investigated whether the dog could possibly be a zoonotic reservoir for the disease in Suriname. Forty-seven dogs were examined for overt clinical signs of leishmaniasis, and blood samples were collected on filter paper for serology (direct agglutination test) and molecular biology (by polymerase chain reaction). Three dogs had clinical signs that could be compatible with canine cutaneous leishmaniosis: dermatitis (two) or nasal lesion (one). Two dogs were seropositive with DAT (titre > 1 : 1600), and three animals had a borderline titre (1 : 800). All other animals (n = 42) were DAT negative. PCR analysis found Leishmania DNA equivalent to 1 parasite per mL in only one dog at a first round of analysis, but this animal was negative after retesting. The clinical, serological, and molecular data show some preliminary lines of evidence that canine leishmaniosis is present in Suriname, but further studies are needed to incriminate the reservoir, including a possible sylvatic cycle.

show abstract

Leishmania (Viannia) Infection in the Domestic Dog in Chaparral, Colombia

Cited by 37 publications

References 23 publications

Animal Models for the Study of Leishmaniasis Immunology

Animal Models for the Study of Leishmaniasis Immunology

Phlebotomine Vector Ecology in the Domestic Transmission of American Cutaneous Leishmaniasis in Chaparral, Colombia

Is the Dog a Possible Reservoir for Cutaneous Leishmaniasis in Suriname?

Contact Info

Product

Resources

About